Neurocan (NCAN) (NM_004386) Human Untagged Clone

CAT#: SC303477

NCAN (untagged)-Human neurocan (NCAN)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NCAN
• Synonyms: CSPG3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004386, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGGGCCCCGTTTGTCTGGGCCTTGGGCCTTTTGATGCTGCAGATGCTGCTCTTTGTGGCTGGGGAAC
  AGGGCACACAGGATATCACCGATGCCAGCGAAAGGGGGCTCCACATGCAGAAGCTGGGGTCTGGGTCAGT
  GCAGGCTGCGCTGGCGGAGCTGGTGGCCCTGCCCTGTCTCTTTACCCTGCAGCCACGGCCAAGCGCAGCC
  CGAGATGCCCCTCGGATAAAGTGGACCAAGGTGCGGACTGCGTCGGGCCAGCGACAGGACTTGCCCATCC
  TGGTGGCCAAGGACAATGTCGTGAGGGTGGCCAAAAGCTGGCAGGGACGAGTGTCACTGCCTTCCTACCC
  CCGGCGCCGAGCCAACGCCACGCTACTTCTGGGGCCACTGAGGGCCAGTGACTCTGGGCTGTACCGCTGC
  CAGGTGGTGAGGGGCATCGAGGATGAGCAGGACCTGGTGCCCTTGGAGGTGACAGGTGTTGTGTTCCACT
  ACCGATCAGCCCGGGACCGCTATGCACTGACCTTCGCTGAGGCCCAGGAGGCCTGCCGTCTCAGCTCAGC
  CATCATTGCAGCCCCTCGGCATCTACAGGCTGCCTTTGAGGATGGCTTTGACAACTGTGATGCTGGCTGG
  CTCTCTGACCGCACTGTTCGGTATCCTATCACCCAGTCCCGTCCTGGTTGCTATGGCGACCGTAGCAGCC
  TTCCAGGGGTTCGGAGCTATGGGAGGCGCAACCCACAGGAACTCTACGATGTGTATTGCTTTGCCCGGGA
  GCTGGGGGGCGAGGTCTTCTACGTGGGCCCGGCCCGCCGCCTGACACTGGCCGGCGCGCGTGCACAGTGC
  CGCCGCCAGGGTGCCGCGCTGGCCTCGGTGGGACAGCTGCACCTGGCCTGGCATGAGGGCCTGGACCAGT
  GCGACCCGGGCTGGCTGGCCGACGGCAGCGTGCGCTACCCGATCCAGACGCCGCGCCGGCGCTGCGGGGG
  CCCAGCCCCGGGCGTGCGCACCGTCTACCGCTTCGCTAACCGGACCGGCTTCCCCTCACCCGCCGAGCGC
  TTCGACGCCTACTGCTTCCGAGCTCATCACCCCACGTCACAACATGGAGACCTAGAGACCCCATCCTCTG
  GGGATGAGGGGGAGATTCTGTCAGCAGAGGGGCCCCCAGTTAGAGAACTGGAGCCCACCCTGGAGGAGGA
  AGAGGTGGTCACCCCTGACTTCCAGGAGCCTCTGGTGTCCAGTGGGGAAGAAGAAACCCTGATTTTGGAG
  GAGAAGCAGGAGTCTCAACAGACCCTCAGCCCTACCCCTGGGGACCCCATGCTGGCCTCATGGCCCACTG
  GGGAAGTGTGGCTAAGCACGGTGGCCCCCAGCCCTAGCGACATGGGGGCAGGCACTGCAGCAAGTTCACA
  CACGGAGGTGGCCCCAACTGACCCTATGCCTAGGAGAAGGGGGCGCTTCAAAGGGTTGAATGGGCGCTAC
  TTCCAGCAGCAGGAACCGGAGCCGGGGCTGCAAGGGGGGATGGAGGCCAGCGCCCAGCCCCCCACCTCAG
  AGGCTGCAGTGAACCAAATGGAGCCTCCGTTGGCCATGGCAGTCACAGAGATGTTGGGCAGTGGCCAGAG
  CCGGAGCCCCTGGGCTGATCTGACCAATGAGGTGGATATGCCTGGAGCTGGTTCTGCTGGTGGCAAGAGC
  TCCCCAGAGCCCTGGCTGTGGCCCCCTACCATGGTCCCACCCAGCATCTCAGGCCACAGCAGGGCCCCTG
  TCCTGGAGCTAGAGAAAGCCGAGGGCCCCAGTGCCAGGCCAGCCACCCCAGACCTGTTTTGGTCCCCCTT
  GGAGGCCACTGTCTCAGCTCCCAGCCCTGCCCCCTGGGAGGCATTCCCTGTGGCCACCTCCCCAGATCTC
  CCTATGATGGCCATGCTGCGTGGTCCCAAAGAGTGGATGCTACCACACCCCACCCCCATCTCCACCGAGG
  CCAATAGAGTTGAGGCACATGGTGAGGCCACCGCCACGGCTCCACCCTCCCCTGCTGCAGAGACCAAGGT
  GTATTCCCTGCCTCTCTCTTTGACCCCAACAGGACAGGGTGGAGAGGCCATGCCCACAACACCTGAGTCC
  CCCAGGGCAGACTTCAGAGAAACTGGGGAGACCAGCCCTGCTCAGGTCAACAAAGCTGAGCACTCCAGCT
  CCAGCCCATGGCCTTCTGTAAACAGGAATGTGGCTGTAGGTTTTGTCCCCACTGAGACTGCCACTGAGCC
  AACGGGCCTCAGGGGTATCCCGGGGTCTGAGTCTGGGGTCTTCGACACAGCAGAAAGCCCCACTTCTGGC
  TTGCAGGCCACTGTAGATGAGGTGCAGGACCCCTGGCCCTCAGTGTACAGCAAAGGGCTGGATGCAAGTT
  CCCCATCTGCCCCCCTGGGGAGCCCTGGAGTCTTCTTGGTACCCAAAGTCACCCCAAATTTGGAGCCTTG
  GGTTGCTACAGATGAAGGACCCACTGTGAATCCCATGGATTCCACAGTCACGCCGGCCCCCAGTGATGCT
  AGTGGAATTTGGGAACCTGGATCCCAGGTGTTTGAAGAAGCCGAAAGCACCACCTTGAGCCCTCAGGTGG
  CCCTGGATACAAGCATTGTGACGCCCCTCACGACCCTGGAGCAGGGGGACAAGGTTGGAGTTCCAGCCAT
  GTCTACACTGGGCTCCTCAAGCTCCCAACCCCACCCAGAGCCAGAGGATCAGGTGGAGACCCAGGGAACA
  TCAGGAGCTTCAGTGCCTCCGCATCAGAGCAGTCCCCTAGGGAAACCGGCTGTTCCTCCTGGGACACCGA
  CTGCAGCCAGTGTGGGCGAGTCTGCCTCAGTTTCCTCAGGGGAGCCTACGGTACCGTGGGACCCCTCCAG
  CACCCTGCTGCCTGTCACCCTGGGCATAGAGGACTTCGAACTGGAGGTCCTGGCAGGGAGCCCGGGTGTA
  GAGAGCTTCTGGGAGGAGGTGGCAAGTGGAGAGGAGCCAGCCCTGCCAGGGACCCCTATGAATGCAGGTG
  CGGAGGAGGTGCACTCAGATCCCTGTGAGAACAACCCTTGTCTTCATGGAGGGACATGTAATGCCAATGG
  CACCATGTATGGCTGTAGCTGTGATCAGGGCTTCGCCGGGGAGAACTGTGAGATTGACATTGATGACTGC
  CTCTGCAGCCCCTGTGAGAATGGAGGCACCTGTATTGATGAGGTCAATGGCTTTGTCTGCCTTTGCCTCC
  CCAGCTATGGGGGCAGCTTTTGTGAGAAAGACACCGAGGGCTGTGACCGCGGCTGGCATAAGTTCCAGGG
  CCACTGTTACCGCTATTTTGCCCACCGGAGGGCATGGGAAGATGCCGAGAAGGACTGCCGCCGCCGCTCC
  GGCCACCTGACCAGCGTCCACTCACCGGAGGAACACAGCTTCATTAATAGCTTTGGGCATGAAAACACGT
  GGATCGGCCTGAACGACAGGATCGTGGAGAGAGATTTCCAGTGGACGGACAACACCGGGCTGCAATTTGA
  GAACTGGCGAGAGAACCAGCCGGACAATTTCTTCGCGGGTGGCGAGGACTGTGTGGTGATGGTGGCGCAT
  GAAAGCGGGCGCTGGAACGATGTCCCCTGCAACTACAACCTACCCTATGTCTGCAAGAAGGGCACAGTGC
  TCTGTGGTCCCCCTCCGGCAGTGGAGAATGCCTCACTCATCGGTGCCCGCAAGGCCAAGTACAATGTCCA
  TGCCACTGTAAGGTACCAGTGCAATGAAGGATTTGCCCAGCACCATGTGGCCACCATTCGATGCCGGAGC
  AATGGCAAGTGGGACAGGCCCCAAATTGTCTGCACCAAACCCAGACGTTCACATCGGATGCGGCGACACC
  ACCACCACCACCAACACCACCACCAGCATCACCACCACAAATCCCGCAAGGAGCGCAGAAAACACAAGAA
  ACACCCAACGGAGGACTGGGAGAAGGACGAAGGGAATTTCTGCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_004386
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004386.2, NP_004377.2
• RefSeq Size: 6406 bp
• RefSeq ORF: 3966 bp
• Locus ID: 1463
• Cytogenetics: 19p13.11
• Gene Summary: 'Neurocan is a chondroitin sulfate proteoglycan thought to be involved in the modulation of cell adhesion and migration.[supplied by OMIM, Jul 2002]'