Tensin 1 (TNS1) (NM_022648) Human Untagged Clone

CAT#: SC305040

TNS1 (untagged)-Human tensin 1 (TNS1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TNS1
• Synonyms: MST091; MST122; MST127; MSTP091; MSTP122; MSTP127; MXRA6; PPP1R155; TNS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_022648, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGTGTGAGCCGGACCATGGAGGACAGCTGTGAGCTGGACCTGGTGTACGTCACAGAGAGGATCATCG
  CTGTCTCCTTCCCCAGCACAGCCAATGAGGAGAACTTCCGGAGCAACCTCCGTGAGGTGGCGCAGATGCT
  CAAGTCCAAACATGGAGGCAACTACCTGCTGTTCAACCTCTCTGAGCGGAGACCTGACATCACGAAGCTC
  CATGCCAAGGTACTGGAATTTGGCTGGCCCGACCTCCACACCCCAGCCCTGGAGAAGATCTGCAGCATCT
  GTAAGGCCATGGACACATGGCTCAATGCAGACCCTCACAATGTCGTTGTTCTACACAACAAGGGAAACCG
  AGGCAGGATAGGAGTTGTCATCGCGGCTTACATGCACTACAGCAACATTTCTGCCAGTGCGGACCAGGCT
  CTGGACCGGTTTGCAATGAAGCGGTTCTATGAGGATAAGATTGTGCCCATTGGCCAGCCATCCCAAAGAA
  GGTACGTGCATTACTTCAGTGGCCTGCTCTCCGGCTCCATCAAAATGAACAACAAGCCCTTGTTTCTGCA
  CCACGTGATCATGCACGGCATCCCCAACTTTGAGTCTAAAGGAGGATGTCGGCCATTTCTCCGCATCTAC
  CAGGCCATGCAACCTGTGTACACATCTGGCATCTACAACATCCCAGGAGACAGCCAGACTAGCGTCTGCA
  TCACCATCGAGCCAGGACTGCTCTTGAAGGGAGACATCTTGCTGAAGTGCTACCACAAGAAGTTCCGAAG
  CCCAGCCCGAGACGTCATCTTCCGTGTGCAGTTCCACACCTGTGCCATCCATGACCTGGGGGTTGTCTTT
  GGGAAGGAGGACCTTGATGATGCTTTCAAAGATGATCGATTTCCAGAGTATGGCAAAGTGGAGTTTGTAT
  TTTCTTATGGGCCAGAGAAAATTCAAGGCATGGAGCACCTGGAGAACGGGCCGAGCGTGTCTGTGGACTA
  TAACACCTCCGACCCCCTCATCCGCTGGGACTCCTACGACAACTTCAGTGGGCATCGAGATGACGGCATG
  GAGGAGGTGGTGGGACACACGCAGGGGCCACTAGATGGGAGCCTGTATGCTAAGGTGAAGAAGAAAGACT
  CCCTGCACGGCAGCACCGGGGCTGTTAATGCCACACGTCCTACACTGTCGGCCACCCCCAACCACGTGGA
  ACACACGCTTTCTGTGAGCAGCGACTCGGGCAACTCCACAGCCTCCACCAAGACCGACAAGACCGACGAG
  CCTGTCCCCGGGGCCTCCAGTGCCACTGCTGCCTTGAGTCCCCAGGAGAAGCGGGAGCTGGACCGCCTGC
  TGAGTGGCTTTGGCTTAGAGCGAGAGAAGCAAGGCGCCATGTACCACACCCAGCACCTCAGGTCCCGCCC
  AGCAGGGGGCTCGGCTGTGCCCTCCTCTGGACGCCACGTTGTCCCAGCCCAGGTTCATGTCAATGGTGGG
  GCGTTAGCATCTGAGCGGGAGACAGACATCCTGGACGATGAATTGCCAAACCAGGATGGTCACAGTGCGG
  GCAGCATGGGCACACTCTCTTCTCTGGACGGGGTCACCAACACCAGTGAGGGGGGCTACCCAGAGGCCCT
  GTCCCCACTGACCAACGGTCTGGACAAGTCCTACCCCATGGAGCCTATGGTCAATGGAGGAGGCTACCCC
  TACGAGTCTGCCAGCCGGGCGGGGCCTGCCCATGCTGGCCACACGGCCCCCATGCGGCCCTCCTACTCTG
  CACAGGAGGGTTTAGCTGGCTACCAGAGGGAGGGGCCCCACCCAGCCTGGCCACAGCCAGTGACCACCTC
  CCACTATGCCCATGACCCCAGCGGTATGTTCCGCTCTCAATCCTTTTCGGAAGCTGAACCCCAGCTGCCC
  CCAGCTCCGGTCCGAGGGGGAAGCAGCCGGGAGGCTGTGCAAAGGGGACTGAATTCGTGGCAGCAGCAGC
  AGCAGCAGCAGCAGCAGCCTCGCCCACCTCCACGCCAGCAGGAAAGAGCCCACTTGGAGAGTCTTGTAGC
  CAGCAGGCCCAGCCCTCAGCCATTGGCAGAGACCCCCATCCCCAGTCTCCCTGAGTTCCCGCGAGCAGCC
  TCCCAGCAGGAGATTGAACAGTCCATCGAAACACTCAATATGCTGATGCTGGACCTGGAGCCAGCCTCCG
  CTGCTGCCCCACTACACAAGTCCCAGAGTGTCCCCGGGGCCTGGCCAGGGGCTTCTCCACTCTCCTCCCA
  GCCCCTCTCTGGATCCTCCCGTCAGTCCCATCCACTGACCCAGTCCAGATCTGGCTATATCCCCAGTGGG
  CATTCGTTGGGAACCCCTGAGCCAGCCCCACGGGCCTCTCTGGAGTCTGTCCCTCCTGGCAGGTCTTACT
  CACCTTATGACTATCAGCCATGTTTGGCTGGGCCTAACCAGGATTTCCATTCAAAGAGCCCAGCCTCTTC
  CTCCTTGCCTGCCTTCCTTCCGACCACCCACAGCCCTCCAGGGCCTCAGCAACCCCCAGCCTCTCTCCCT
  GGCCTCACTGCTCAGCCTCTGCTCTCACCAAAGGAAGCGACTTCAGACCCCTCCCGGACTCCAGAGGAGG
  AGCCATTGAATTTAGAAGGGCTGGTGGCCCACAGGGTAGCAGGGGTACAGGCTCGGGAGAAGCAGCCTGC
  AGAGCCCCCAGCCCCTCTGCGGAGGCGGGCGGCCAGTGATGGACAGTATGAGAACCAGTCTCCAGAAGCC
  ACATCCCCTCGTAGCCCTGGGGTTCGCTCCCCTGTCCAGTGTGTCTCCCCGGAGCTGGCTCTTACCATCG
  CTCTCAATCCTGGAGGGCGGCCCAAAGAGCCCCATTTGCACAGCTACAAGGAGGCCTTCGAGGAGATGGA
  GGGAACCTCCCCGAGCAGCCCACCACCCAGTGGGGTGCGGTCCCCCCCGGGTCTGGCCAAGACACCCCTG
  TCTGCTCTGGGCCTGAAACCTCACAACCCAGCGGACATCCTGTTGCACCCCACAGGAGTTACCAGAAGAC
  GGATCCAGCCAGAGGAAGATGAGGGGAAGGTGGTGGTCAGGCTGTCAGAAGAGCCCCGGAGCTATGTGGA
  GTCTGTGGCACGGACAGCGGTGGCTGGACCCCGAGCTCAGGACTCTGAGCCCAAGAGCTTTAGTGCTCCA
  GCCACCCAGGCCTATGGCCATGAGATACCCCTGAGGAACGGGACCCTGGGTGGCTCCTTTGTCTCCCCCA
  GCCCCCTCTCCACCAGCAGCCCCATCCTCAGTGCTGACAGCACTTCAGTGGGGAGTTTCCCGTCGGGAGA
  GAGCAGTGACCAGGGTCCCCGGACGCCCACCCAGCCTCTGTTGGAGTCTGGCTTCCGCTCAGGCAGCCTG
  GGACAGCCCAGCCCGTCTGCCCAGAGAAACTACCAGAGCTCTTCTCCTCTCCCGACTGTGGGCAGTAGCT
  ACAGCAGCCCCGACTACTCACTTCAGCATTTCAGCTCCTCTCCGGAAAGCCAGGCTCGAGCTCAGTTCAG
  TGTGGCTGGCGTCCACACGGTGCCTGGGAGCCCTCAGGCGCGCCACAGAACAGTGGGCACCAACACTCCC
  CCTAGTCCTGGCTTCGGCTGGCGGGCCATCAATCCCAGCATGGCTGCCCCCAGCAGTCCCAGTTTGAGCC
  ATCACCAGATGATGGGTCCACCAGGCACTGGCTTCCATGGTAGCACTGTCTCCAGCCCCCAGAGCAGTGC
  AGCGACCACCCCGGGGAGCCCCAGCCTGTGTCGGCACCCAGCAGGGGTCTACCAGGTTTCTGGCCTCCAC
  AACAAAGTGGCCACCACCCCGGGGAGTCCCAGCCTGGGCCGGCACCCTGGGGCTCACCAAGGCAACCTGG
  CCTCCGGTCTTCATAGCAATGCAATAGCCAGCCCTGGAAGCCCCAGCCTGGGCCGTCACCTCGGAGGGTC
  TGGATCTGTGGTTCCCGGCAGCCCCTGCTTGGACCGGCATGTGGCCTATGGTGGCTATTCTACCCCGGAG
  GATCGGAGACCCACACTGTCCCGGCAGAGCAGTGCCTCTGGCTACCAGGCTCCTTCCACGCCCTCCTTCC
  CTGTCTCCCCTGCCTACTACCCTGGCCTGAGCAGCCCTGCCACCTCCCCGTCACCAGACTCCGCAGCCTT
  CCGGCAAGGGAGCCCAACACCAGCCTTGCCAGAGAAGCGAAGGATGTCAGTGGGAGACCGGGCAGGCAGC
  CTCCCCAACTATGCCACCATCAATGGGAAGGTGTCTTCGCCTGTCGCCAGCGGCATGTCCAGTCCCAGTG
  GGGGCAGCACCGTCTCCTTCTCCCACACTCTGCCCGACTTCTCCAAGTACTCCATGCCAGACAACAGCCC
  GGAGACGCGGGCTAAAGTGAAGTTTGTCCAGGACACTTCTAAGTATTGGTACAAGCCTGAGATCTCCAGG
  GAGCAGGCCATCGCGCTCCTCAAGGACCAGGAGCCGGGGGCCTTCATCATCCGCGACAGTCACTCCTTCC
  GAGGCGCGTACGGGCTGGCCATGAAGGTGTCTTCGCCACCTCCAACCATCATGCAGCAGAATAAAAAAGG
  AGACATGACCCATGAGCTGGTCAGGCATTTTCTGATAGAGACTGGCCCCAGAGGAGTCAAGCTCAAGGGC
  TGCCCCAATGAGCCAAACTTCGGATCGCTGTCTGCCCTGGTCTACCAGCACTCCATCATCCCATTGGCCC
  TGCCTTGCAAGCTGGTCATTCCAAACCGAGACCCCACAGATGAATCGAAAGATAGCTCCGGCCCTGCCAA
  CTCAACTGCAGACCTGCTGAAACAAGGGGCAGCCTGCAATGTGCTCTTCGTCAACTCTGTGGACATGGAG
  TCACTCACTGGGCCACAGGCCATCTCTAAAGCCACATCTGAGACGTTGGCTGCAGACCCCACGCCAGCTG
  CCACCATCGTTCACTTCAAAGTCTCTGCCCAGGGAATCACTCTGACTGACAACCAGAGAAAGCTCTTTTT
  CAGACGCCACTACCCTCTCAACACTGTCACCTTCTGTGACCTGGATCCACAGGAAAGAAAGTGGATGAAA
  ACAGAGGGTGGTGCCCCTGCTAAGCTCTTCGGCTTCGTGGCCCGGAAGCAGGGCAGCACCACGGACAACG
  CCTGCCACCTCTTTGCTGAGCTTGACCCCAACCAGCCGGCCTCTGCCATCGTCAACTTCGTCTCCAAGGT
  CATGCTGAATGCCGGCCAAAAGAGATGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_022648
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022648.5, NP_072174.3
• RefSeq Size: 10294 bp
• RefSeq ORF: 5208 bp
• Locus ID: 7145
• Cytogenetics: 2q35
• Protein Families: Druggable Genome
• Gene Summary: 'The protein encoded by this gene localizes to focal adhesions, regions of the plasma membrane where the cell attaches to the extracellular matrix. This protein crosslinks actin filaments and contains a Src homology 2 (SH2) domain, which is often found in molecules involved in signal transduction. This protein is a substrate of calpain II. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and Northern Blot.