DDX4 (NM_024415) Human Untagged Clone

CAT#: SC305113

DDX4 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (DDX4), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DDX4
• Synonyms: VASA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_024415, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGAGATGAAGATTGGGAAGCAGAAATCAACCCTCATATGTCTTCCTATGTTCCCATATTTGAGAAGG
  ATAGGTATTCTGGAGAAAATGGAGACAATTTTAACAGGACTCCAGCTTCATCATCAGAAATGGATGATGG
  ACCTTCTCGAAGAGATCATTTCATGAAAAGTGGATTTGCCTCTGGGCGGAATTTTGGAAACAGAGATGCT
  GGTGAGTGTAATAAGCGAGATAATACATCCACAATGGGTGGTTTTGGAGTTGGAAAGAGTTTTGGAAACA
  GAGGTTTTTCAAACAGCAGGTTTGAAGATGGTGATAGCTCTGGTTTCTGGAGAGAGTCTAGTAATGACTG
  CGAAGATAATCCAACACGGAACAGAGGGTTTTCCAAGAGAGGCGGCTATCGAGATGGAAATAATTCAGAA
  GCTTCAGGGCCATACAGAAGAGGTGGAAGAGGTAGTTTCCGAGGTTGCCGTGGAGGATTTGGTCTAGGAA
  GTCCAAATAATGACTTAGACCCAGACGAATGTATGCAGCGCACTGGTGGCCTTTTTGGTTCTAGAAGACC
  AGTATTAAGTGGCACAGGTAATGGTGATACTTCTCAAAGCAGAAGTGGCAGTGGAAGTGAACGAGGTGGT
  TACAAAGGTTTAAATGAAGAAGTAATAACAGGCTCTGGAAAGAATTCTTGGAAGTCAGAAGCAGAAGGAG
  GAGAAAGTAGTGATACTCAAGGACCAAAAGTGACCTACATACCCCCTCCTCCACCTGAGGATGAGGACTC
  CATCTTTGCACATTATCAGACAGGCATAAACTTCGACAAATACGACACTATTCTTGTGGAAGTGTCTGGA
  CATGATGCACCACCAGCAATTCTGACTTTTGAAGAAGCTAATCTCTGTCAGACACTGAATAACAACATTG
  CTAAAGCTGGTTATACTAAGCTTACTCCTGTGCAAAAATACAGTATTCCTATCATACTTGCAGGACGAGA
  TTTGATGGCTTGCGCTCAAACAGGGTCTGGGAAGACTGCGGCTTTTCTCCTACCAATTTTGGCTCATATG
  ATGCATGATGGAATAACTGCCAGTCGTTTTAAAGAGTTGCAGGAACCAGAGTGTATTATTGTAGCACCAA
  CTCGAGAATTGGTCAACCAGATTTATTTGGAAGCCAGAAAATTTTCTTTTGGGACTTGTGTAAGAGCTGT
  TGTTATATATGGGGGAACCCAGCTGGGACATTCAATTCGACAAATAGTACAAGGCTGTAATATATTATGT
  GCTACTCCTGGAAGACTGATGGATATCATAGGCAAAGAAAAGATTGGTCTCAAACAGATCAAATACTTAG
  TTTTGGATGAAGCTGATCGCATGTTGGATATGGGTTTTGGTCCAGAAATGAAGAAGTTAATTTCTTGCCC
  AGGAATGCCATCAAAGGAACAGCGCCAAACCCTTATGTTCAGTGCAACTTTTCCAGAGGAAATTCAAAGG
  TTGGCTGCAGAGTTTTTAAAGTCAAATTATCTGTTTGTTGCTGTTGGACAAGTGGGTGGAGCATGTAGAG
  ATGTTCAGCAGACCGTTCTCCAAGTTGGCCAGTTCTCAAAAAGAGAAAAGCTCGTTGAAATTCTGCGAAA
  CATAGGGGATGAAAGAACTATGGTCTTTGTTGAAACTAAGAAAAAAGCAGATTTTATTGCAACTTTTCTT
  TGTCAAGAAAAAATATCAACTACAAGTATTCATGGTGATCGGGAACAGAGAGAGCGGGAGCAAGCTCTTG
  GAGATTTTCGCTTTGGAAAGTGCCCAGTTCTTGTTGCTACTTCAGTAGCTGCCAGAGGGCTGGATATTGA
  AAATGTGCAACATGTTATCAATTTTGATCTTCCTTCTACCATTGATGAATATGTTCATCGAATTGGGCGT
  ACTGGTCGTTGTGGGAATACTGGCAGAGCAATTTCCTTTTTTGATCTTGAATCGGATAACCATTTAGCAC
  AGCCTCTAGTAAAAGTATTGACAGATGCTCAACAGGATGTTCCTGCATGGTTGGAAGAAATTGCCTTTAG
  TACATACATTCCTGGCTTCAGTGGTAGTACAAGAGGAAACGTGTTTGCATCAGTTGATACCAGAAAGGGC
  AAGAGCACTTTGAACACAGCTGGGTTTTCTTCTTCACAAGCTCCCAATCCAGTAGATGATGAGTCATGGG
  ATTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_024415
• ORF Size: 2175 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_024415.2, NP_077726.1
• RefSeq Size: 2880
• RefSeq ORF: 2175
• Locus ID: 54514
• Gene Summary: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
  Transcript Variant: This variant (1) encodes the longest isoform (1).