MAGEA2 (NM_175742) Human Untagged Clone

CAT#: SC307027

MAGEA2 (untagged)-Human melanoma antigen family A, 2 (MAGEA2), transcript variant 2


  "NM_175742" in other vectors (5)

Reconstitution Protocol

USD 660.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol MAGEA2
Synonyms CT1.2; MAGE2; MAGEA2A
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_175742 edited
GGTTCTGAGGGGGACAGGCTGACAAGTAGGACCCGAGGCACTGGAGGAGCATTGAAGGAG
AAGATCTGCCTGTGGGTCTTCATTGCCCAGCTCCTGCCCGCACTCCTGCCTGCTGCCCTG
ACCAGAGTCATCATGCCTCTTGAGCAGAGGAGTCAGCACTGCAAGCCTGAAGAAGGCCTT
GAGGCCCGAGGAGAGGCCCTGGGCCTGGTGGGTGCGCAGGCTCCTGCTACTGAGGAGCAG
CAGACCGCTTCTTCCTCTTCTACTCTAGTGGAAGTTACCCTGGGGGAGGTGCCTGCTGCC
GACTCACCGAGTCCTCCCCACAGTCCTCAGGGAGCCTCCAGCTTCTCGACTACCATCAAC
TACACTCTTTGGAGACAATCCGATGAGGGCTCCAGCAACCAAGAAGAGGAGGGGCCAAGA
ATGTTTCCCGACCTGGAGTCCGAGTTCCAAGCAGCAATCAGTAGGAAGATGGTTGAGTTG
GTTCATTTTCTGCTCCTCAAGTATCGAGCCAGGGAGCCGGTCACAAAGGCAGAAATGCTG
GAGAGTGTCCTCAGAAATTGCCAGGACTTCTTTCCCGTGATCTTCAGCAAAGCCTCCGAG
TACTTGCAGCTGGTCTTTGGCATTGAGGTGGTGGAAGTGGTCCCCATCAGCCACTTGTAC
ATCCTTGTCACCTGCCTGGGCCTCTCCTACGATGGCCTGCTGGGCGACAATCAGGTCATG
CCCAAGACAGGCCTCCTGATAATCGTCCTGGCCATAATCGCAATAGAGGGCGACTGTGCC
CCTGAGGAGAAAATCTGGGAGGAGCTGAGTATGTTGGAGGTGTTTGAGGGGAGGGAGGAC
AGTGTCTTCGCACATCCCAGGAAGCTGCTCATGCAAGATCTGGTGCAGGAAAACTACCTG
GAGTACCGGCAGGTGCCCGGCAGTGATCCTGCATGCTACGAGTTCCTGTGGGGTCCAAGG
GCCCTCATTGAAACCAGCTATGTGAAAGTCCTGCACCATACACTAAAGATCGGTGGAGAA
CCTCACATTTCCTACCCACCCCTGCATGAACGGGCTTTGAGAGAGGGAGAAGAGTGAGTC
TCAGCACATGTTGCAGCCAGGGCCAGTGGGAGGGGGTCTGGGCCAGTGCACCTTCCAGGG
CCCCATCCATTAGCTTCCACTGCCTCGTGTGATATGAGGCCCATTCCTGCCTCTTTGAAG
AGAGCAGTCAGCATTCTTAGCAGTGAGTTTCTGTTCTGTTGGATGACTTTGAGATTTATC
TTTGTTTCCTGTTGGAATTGTTCAAATGTTCCTTTTAACAAATGGTTGGATGAACTTCAG
CATCCAAGTTTATGAATGACAGTAGTCACACATAGTGCTGTTTATATAGTTTAGGGGTAA
GAGTCCTGTTTTTTATTCAGATTGGGAAATCCATTCCATTTTGTGAGTTGTCACATAATA
ACAGCAGTGGAATATGTATTTGCCTATATTGTGAACGAATTAGCAGTAAAATACATGATA
CAAGGAACTCAAAAGATAGTTAATTCTTGCCTTATACCTCAGTCTATTATGTAAAATTAA
AAATATGTGTATGTTTTTGCTTCTTTGAGAATGCAAAAGAAATTAAATCTGAATAAAAAA
AAAAAAAAA
Restriction Sites Please inquire     
ACCN NM_175742
Insert Size 1600 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The open reading frame of this clone has been fully sequenced and found to be a perfect match to the protein associated with this reference, NM_175742.1.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_175742.1, NP_786884.1
RefSeq Size 1965 bp
RefSeq ORF 945 bp
Locus ID 4101
Cytogenetics Xq28
Gene Summary 'This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. This gene has two identical copies at different loci. Alternatively spliced transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (2) has an additional exon in the 5' region and lacks an alternate exon in a downstream region, compared to variant 3. Variants 1 through 7 encode the same protein.

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