Amino terminal enhancer of split (AES) (NM_198969) Human Untagged Clone

CAT#: SC307835

AES (untagged)-Human amino-terminal enhancer of split (AES), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TLE5
• Synonyms: AES; AES-1; AES-2; ESP1; GRG; Grg-5; GRG5
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_198969, the custom clone sequence may differ by one or more nucleotides
  ATGTGTCACAAGAATGGCTTCCCTCAGGAAGGCGGCATTACCGCTGCCTTTCTGCAGAAA
  AGGAAACTAAGGCTCAGCAAGAACCACCGCCCAGCCAGAGCCAAGGTCACAGAGCACGTC
  CGTGGCACGCGTCCAGGTCGTGCCACAGCAGGGCCGGCGGCTTCGACGCGGGCAGCCGGG
  TCCCTTTTCTTTGACAGATGGGGAAACCGAGGCCCGGCCGGCTGCCGGGGCTCCTCGCAC
  CTACCCCAGCAACTCAAATTCACCACCTCGGACTCCTGCGACCGCATCAAAGACGAATTT
  CAGCTACTGCAAGCTCAGTACCACAGCCTCAAGCTCGAATGTGACAAGTTGGCCAGTGAG
  AAGTCAGAGATGCAGCGTCACTATGTGATGTACTACGAGATGTCCTACGGCTTGAACATC
  GAGATGCACAAACAGGCTGAGATCGTCAAAAGGCTGAACGGGATTTGTGCCCAGGTCCTG
  CCCTACCTCTCCCAAGAGCACCAGCAGCAGGTCTTGGGAGCCATTGAGAGGGCCAAGCAG
  GTCACCGCTCCCGAGCTGAACTCTATCATCCGACAGCAGCTCCAAGCCCACCAGCTGTCC
  CAGCTGCAGGCCCTGGCCCTGCCCTTGACCCCACTACCCGTGGGGCTGCAGCCGCCTTCG
  CTGCCGGCGGTCAGCGCAGGCACCGGCCTCCTCTCGCTGTCCGCGCTGGGTTCCCAGGCC
  CACCTCTCCAAGGAAGACAAGAACGGGCACGATGGTGACACCCACCAGGAGGATGATGGC
  GAGAAGTCGGATTAG
  
• Restriction Sites: Please inquire
• ACCN: NM_198969
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198969.1, NP_945320.1
• RefSeq Size: 1754 bp
• RefSeq ORF: 795 bp
• Locus ID: 166
• Cytogenetics: 19p13.3
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'The protein encoded by this gene is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein involved in neurogenesis during embryonic development. The encoded protein, which belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).