MYBPC1 (NM_206821) Human Untagged Clone

CAT#: SC308268

MYBPC1 (untagged)-Human myosin binding protein C, slow type (MYBPC1), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MYBPC1
• Synonyms: LCCS4; MYBPCC; MYBPCS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_206821, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCAGAACCCACTAAGAAAGAGGAAAATGAAGTGCCAGCCCCAGCCCCACCCCCGGAAGAACCAAGTA
  AAGAGAAGGAGGCCGGAACTACACCAGCAAAAGACTGGACCCTTGTCGAAACTCCTCCTGGGGAGGAACA
  AGCCAAGCAGAATGCCAACTCCCAGCTGTCCATCTTGTTCATTGAAAAACCTCAAGGAGGAACAGTGAAA
  GTTGGTGAAGATATCACCTTCATAGCCAAAGTCAAGGCTGAAGATCTTCTGAGAAAACCCACTATCAAAT
  GGTTCAAAGGAAAATGGATGGACCTGGCCAGCAAAGCCGGGAAGCACCTTCAGCTGAAGGAAACCTTTGA
  GAGGCACAGTCGGGTGTACACATTTGAGATGCAGATCATCAAGGCCAAAGATAACTTTGCAGGAAATTAC
  AGATGCGAGGTCACCTATAAGGATAAGTTTGACAGCTGTTCATTTGATCTTGAAGTGCACGAATCTACTG
  GGACTACTCCAAACATTGACATCAGATCTGCTTTCAAGAGAAGTGGAGAAGGTCAAGAGGATGCAGGAGA
  ACTTGACTTTAGTGGTCTCCTGAAACGTAGGGAGGTGAAGCAGCAGGAGGAAGAACCCCAGGTGGACGTA
  TGGGAGTTGCTGAAGAACGCGAAACCCAGTGAGTACGAGAAGATCGCCTTCCAGTATGGAATCACCGACC
  TGCGCGGCATGCTCAAGCGACTCAAGCGCATGCGCAGAGAGGAGAAGAAGAGCGCAGCTTTTGCAAAAAT
  TCTTGATCCTGCATATCAGGTTGACAAAGGAGGCAGAGTGAGGTTTGTTGTGGAGCTGGCAGATCCAAAG
  TTGGAGGTGAAATGGTATAAAAATGGTCAAGAAATTCGACCCAGTACCAAATACATCTTTGAACACAAAG
  GATGCCAGAGAATCCTGTTTATCAATAACTGTCAGATGACAGATGATTCAGAGTATTATGTGACAGCCGG
  TGATGAGAAATGTTCCACTGAGCTCTTCGTAAGAGAGCCTCCAATTATGGTGACCAAACAGCTGGAAGAT
  ACAACTGCTTATTGTGGGGAGAGAGTGGAATTAGAATGTGAGGTGTCTGAAGATGATGCCAATGTAAAAT
  GGTTTAAGAATGGTGAAGAGATTATCCCTGGTCCAAAATCAAGATACCGAATTAGAGTTGAGGGTAAAAA
  ACACATCTTGATCATAGAGGGAGCAACAAAGGCTGATGCTGCAGAATATTCAGTAATGACAACAGGAGGA
  CAATCATCTGCTAAACTTAGTGTTGACTTGAAACCTCTGAAGATTTTGACACCTCTGACTGATCAGACTG
  TAAATCTTGGAAAAGAAATCTGCCTGAAGTGTGAAATCTCTGAAAACATACCAGGAAAATGGACTAAAAA
  TGGCCTACCTGTTCAGGAGAGTGACCGTCTAAAGGTGGTTCACAAGGGAAGGATCCACAAGTTAGTGATA
  GCCAATGCCCTCACTGAAGATGAAGGTGATTATGTATTTGCACCTGATGCCTACAATGTTACTCTGCCTG
  CCAAAGTTCATGTTATTGATCCTCCTAAGATCATCCTGGATGGTCTTGATGCTGACAACACAGTGACAGT
  GATTGCAGGAAACAAGCTTCGTCTTGAGATCCCCATCAGCGGAGAACCACCTCCTAAAGCCATGTGGAGC
  CGGGGAGATAAGGCTATTATGGAAGGCAGTGGCCGGATAAGAACAGAATCTTACCCTGATAGCAGCACTC
  TGGTCATTGATATAGCTGAAAGAGATGACTCTGGTGTTTACCACATCAATCTGAAAAACGAAGCTGGAGA
  GGCACATGCAAGCATCAAGGTTAAAGTTGTGGACTTCCCTGATCCTCCAGTGGCACCGACTGTGACAGAG
  GTGGGAGATGACTGGTGTATCATGAACTGGGAGCCTCCTGCCTACGACGGAGGCTCTCCAATCCTAGGAT
  ATTTTATTGAGAGGAAGAAGAAACAAAGCTCCAGGTGGATGAGGCTGAATTTTGATCTCTGCAAAGAAAC
  AACTTTTGAGCCCAAGAAGATGATTGAAGGTGTGGCCTATGAGGTCCGCATCTTTGCAGTCAATGCCATT
  GGCATCTCCAAGCCCAGTATGCCCTCCAGGCCTTTTGTTCCTTTGGCTGTAACAAGCCCTCCTACTCTTC
  TGACTGTGGACTCTGTCACTGACACGACTGTCACGATGAGGTGGCGCCCCCCAGACCACATTGGTGCAGC
  AGGTTTAGATGGCTATGTGCTAGAGTATTGCTTTGAAGGAACTGAGGACTGGATAGTTGCAAACAAAGAT
  CTGATTGACAAGACGAAGTTCACCATCACAGGTCTGCCAACAGATGCAAAGATCTTTGTGCGTGTGAAGG
  CTGTTAATGCAGCTGGTGCCAGCGAGCCCAAGTACTATTCTCAGCCCATTCTCGTGAAGGAAATCATAGA
  ACCTCCAAAGATTCGCATTCCAAGACACCTGAAGCAAACCTATATCCGCAGAGTTGGAGAAGCTGTCAAT
  CTGGTTATACCTTTCCAGGGAAAACCAAGACCAGAATTAACTTGGAAGAAGGATGGTGCAGAAATTGATA
  AGAATCAAATAAACATTCGCAACTCTGAGACTGATACAATCATATTTATTAGAAAAGCAGAGAGGAGCCA
  CTCTGGGAAATATGATCTGCAAGTCAAAGTGGACAAATTCGTGGAGACCGCATCAATTGACATCCAGATC
  ATTGACCGTCCAGGTCCACCCCAAATTGTGAAGATTGAGGATGTCTGGGGAGAAAATGTCGCTCTCACAT
  GGACTCCACCAAAGGATGATGGAAATGCTGCTATCACAGGCTATACCATTCAGAAGGCTGACAAGAAGAG
  CATGGAATGGTTTACTGTCATTGAGCATTATCATCGAACCAGTGCCACCATTACTGAATTGGTCATAGGG
  AATGAATATTACTTCCGGGTCTTTTCTGAAAACATGTGTGGCCTCAGTGAGGATGCCACCATGACTAAAG
  AGAGTGCAGTGATCGCCAGGGATGGTAAAATCTACAAAAATCCAGTGTATGAAGACTTTGATTTCTCAGA
  GGCACCCATGTTTACTCAGCCTTTGGTTAACACCTATGCCATAGCTGGTTACAATGCCACCCTAAACTGC
  AGTGTGAGAGGAAATCCTAAGCCTAAAATAACCTGGATGAAAAACAAAGTTGCTATTGTGGATGATCCAA
  GATACAGGATGTTCAGCAACCAGGGAGTCTGTACCCTGGAAATTCGCAAGCCCAGCCCCTATGATGGAGG
  CACTTACTGCTGCAAAGCAGTCAATGACCTTGGGACAGTGGAGATTGAATGCAAACTGGAGGTGAAAGTC
  ATTGCACAATAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_206821
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_206821.2, NP_996557.1
• RefSeq Size: 3860 bp
• RefSeq ORF: 3372 bp
• Locus ID: 4604
• Cytogenetics: 12q23.2
• Gene Summary: 'This gene encodes a member of the myosin-binding protein C family. Myosin-binding protein C family members are myosin-associated proteins found in the cross-bridge-bearing zone (C region) of A bands in striated muscle. The encoded protein is the slow skeletal muscle isoform of myosin-binding protein C and plays an important role in muscle contraction by recruiting muscle-type creatine kinase to myosin filaments. Mutations in this gene are associated with distal arthrogryposis type I. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]'
  Transcript Variant: This variant (4) lacks three exons in the coding region compared to variant 1. The encoded isoform (4) is shorter and has a distinct C-terminus, compared to variant 1.