PKN1 (NM_213560) Human Untagged Clone

CAT#: SC308656

PKN1 (untagged)-Human protein kinase N1 (PKN1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PKN1
• Synonyms: DBK; PAK-1; PAK1; PKN; PKN-ALPHA; PRK1; PRKCL1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_213560, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGAGGCCAATAACCCCTCGGAGCAGGAGCTGGAGAGTGAGCCTCGCAGCTGGTCCCTGCTAGAGC
  AGCTGGGCCTGGCCGGGGCAGACCTGGCGGCCCCCGGGGTACAGCAGCAGCTGGAGCTGGAGCGGGAGCG
  GCTGCGGCGGGAAATCCGCAAGGAGCTGAAGCTGAAGGAGGGTGCTGAGAACCTGCGGCGGGCCACCACT
  GACCTGGGCCGCAGCCTGGGCCCCGTAGAGCTGCTGCTGCGGGGCTCCTCGCGCCGCCTCGACCTGCTGC
  ACCAGCAGCTGCAGGAGCTGCACGCCCACGTGGTGCTTCCCGACCCGGCGGCCACCCACGATGGCCCCCA
  GTCCCCTGGTGCGGGTGGCCCCACCTGCTCGGCCACCAACCTGAGCCGCGTGGCGGGCCTGGAGAAGCAG
  TTGGCCATTGAGCTGAAGGTGAAGCAGGGGGCGGAGAACATGATCCAGACCTACAGCAATGGCAGCACCA
  AGGACCGGAAGCTGCTGCTGACAGCCCAGCAGATGTTGCAGGACAGTAAGACCAAGATTGACATCATCCG
  CATGCAACTCCGCCGGGCGCTGCAGGCCGGCCAGCTGGAGAACCAGGCAGCCCCGGATGACACCCAAGGG
  AGTCCTGACCTGGGGGCTGTGGAGCTGCGCATCGAAGAGCTGCGGCACCACTTCCGAGTGGAGCACGCGG
  TGGCCGAGGGTGCCAAGAACGTACTGCGCCTGCTCAGCGCTGCCAAGGCCCCGGACCGCAAGGCAGTCAG
  CGAGGCCCAGGAGAAATTGACAGAATCCAACCAGAAGCTGGGGCTGCTGCGGGAGGCTCTGGAGCGGAGA
  CTTGGGGAGCTGCCCGCCGACCACCCCAAGGGGCGGCTGCTGCGAGAAGAGCTCGCTGCGGCCTCCTCCG
  CTGCCTTCAGCACCCGCCTGGCCGGGCCCTTTCCCGCCACGCACTACAGCACCCTGTGCAAGCCCGCGCC
  GCTCACAGGGACCCTGGAGGTACGAGTGGTGGGCTGCAGAGACCTCCCAGAGACCATCCCGTGGAACCCT
  ACCCCCTCAATGGGGGGACCTGGGACCCCAGACAGCCGCCCCCCCTTCCTGAGCCGCCCAGCCCGGGGCC
  TTTACAGCCGAAGCGGAAGCCTCAGTGGCCGGAGCAGCCTCAAAGCAGAAGCCGAGAACACCAGTGAAGT
  CAGCACTGTGCTTAAGCTGGATAACACAGTGGTGGGGCAGACGTCTTGGAAGCCATGTGGCCCCAATGCC
  TGGGACCAGAGCTTCACTCTGGAGCTGGAAAGGGCACGGGAACTGGAGTTGGCTGTGTTCTGGCGGGACC
  AGCGGGGCCTGTGTGCCCTCAAATTCCTGAAGTTGGAGGATTTCTTGGACAATGAGAGGCATGAGGTGCA
  GCTGGACATGGAACCCCAGGGCTGCCTGGTGGCTGAGGTCACCTTCCGCAACCCTGTCATTGAGAGGATT
  CCTCGGCTCCGACGGCAGAAGAAAATTTTCTCCAAGCAGCAAGGGAAGGCGTTCCAGCGTGCTAGGCAGA
  TGAACATCGATGTCGCCACGTGGGTGCGGCTGCTCCGGAGGCTCATCCCCAATGCCACGGGCACAGGCAC
  CTTTAGCCCTGGGGCTTCTCCAGGATCCGAGGCCCGGACCACGGGTGACATATCGGTGGAGAAGCTGAAC
  CTCGGCACTGACTCGGACAGCTCACCTCAGAAGAGCTCGCGGGATCCTCCTTCCAGCCCATCGAGCCTGA
  GCTCCCCCATCCAGGAATCCACTGCTCCCGAGCTGCCTTCGGAGACCCAGGAGACCCCAGGCCCCGCCCT
  GTGCAGCCCTCTGAGGAAGTCACCTCTGACCCTCGAAGATTTCAAGTTCCTGGCGGTGCTGGGCCGGGGT
  CATTTTGGGAAGGTGCTCCTCTCCGAATTCCGGCCCAGTGGGGAGCTGTTCGCCATCAAGGCTCTGAAGA
  AAGGGGACATTGTGGCCCGAGACGAGGTGGAGAGCCTGATGTGTGAGAAGCGGATATTGGCGGCAGTGAC
  CAGTGCGGGACACCCCTTCCTGGTGAACCTCTTCGGCTGTTTCCAGACACCGGAGCACGTGTGCTTCGTG
  ATGGAGTACTCGGCCGGTGGGGACCTGATGCTGCACATCCACAGCGACGTGTTCTCTGAGCCCCGTGCCA
  TCTTTTATTCCGCCTGCGTGGTGCTGGGCCTACAGTTTCTTCACGAACACAAGATCGTCTACAGGGACCT
  GAAGTTGGACAATTTGCTCCTGGACACCGAGGGCTACGTCAAGATCGCAGACTTTGGCCTCTGCAAGGAG
  GGGATGGGCTATGGGGACCGGACCAGCACATTCTGTGGGACCCCGGAGTTCCTGGCCCCTGAGGTGCTGA
  CGGACACGTCGTACACGCGAGCTGTGGACTGGTGGGGACTGGGTGTGCTGCTCTACGAGATGCTGGTTGG
  CGAGTCCCCATTCCCAGGGGATGATGAGGAGGAGGTCTTCGACAGCATCGTCAACGACGAGGTTCGCTAC
  CCCCGCTTCCTGTCGGCCGAAGCCATCGGCATCATGAGAAGGCTGCTTCGGAGGAACCCAGAGCGGAGGC
  TGGGATCTAGCGAGAGAGATGCAGAAGATGTGAAGAAACAGCCCTTCTTCAGGACTCTGGGCTGGGAAGC
  CCTGTTGGCCCGGCGCCTGCCACCGCCCTTTGTGCCCACGCTGTCCGGCCGCACCGACGTCAGCAACTTC
  GACGAGGAGTTCACCGGGGAGGCCCCCACACTGAGCCCGCCCCGCGACGCGCGGCCCCTCACAGCCGCGG
  AGCAGGCAGCCTTCCTGGACTTCGACTTCGTGGCCGGGGGCTGCTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_213560
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_213560.1, NP_998725.1
• RefSeq Size: 2964 bp
• RefSeq ORF: 2847 bp
• Locus ID: 5585
• Cytogenetics: 19p13.12
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: 'The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes the longer isoform (1).