CLIP170 (CLIP1) (NM_198240) Human Untagged Clone

CAT#: SC309360

CLIP1 (untagged)-Human CAP-GLY domain containing linker protein 1 (CLIP1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CLIP1
• Synonyms: CLIP; CLIP-170; CLIP170; CYLN1; RSN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_198240, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGTATGCTAAAGCCAAGTGGGCTTAAGGCCCCCACCAAGATCCTGAAGCCTGGAAGCACAGCTCTGA
  AGACACCTACGGCTGTTGTAGCTCCAGTAGAAAAAACCATATCCAGTGAAAAAGCATCAAGCACTCCATC
  ATCTGAGACTCAGGAGGAATTTGTGGATGACTTTCGAGTTGGGGAGCGAGTTTGGGTGAATGGAAATAAG
  CCTGGATTTATCCAGTTTCTTGGAGAAACCCAGTTTGCACCAGGCCAGTGGGCTGGAATTGTTTTAGATG
  AACCCATAGGCAAGAACGATGGTTCGGTGGCAGGAGTTCGGTATTTCCAGTGTGAACCTTTAAAGGGCAT
  ATTTACCCGACCTTCAAAGTTAACAAGGAAGGTGCAAGCAGAAGATGAAGCTAATGGCCTGCAGACAACG
  CCCGCCTCCCGAGCTACTTCACCGCTGTGCACTTCTACGGCCAGCATGGTGTCTTCCTCCCCCTCCACCC
  CTTCAAACATCCCTCAGAAACCATCACAGCCAGCAGCAAAGGAACCTTCAGCTACGCCTCCGATCAGCAA
  CCTTACAAAAACTGCCAGTGAATCTATCTCCAACCTTTCAGAGGCTGGCTCAATCAAGAAAGGAGAAAGA
  GAGCTCAAAATCGGAGACAGAGTATTGGTTGGTGGCACTAAGGCTGGTGTAGTCCGGTTTCTTGGGGAGA
  CCGACTTTGCCAAGGGGGAGTGGTGTGGCGTGGAGTTAGATGAGCCACTTGGGAAGAATGATGGCGCTGT
  TGCTGGAACAAGGTATTTTCAGTGTCAACCCAAATATGGCTTGTTCGCTCCTGTCCACAAAGTTACCAAG
  ATTGGCTTCCCTTCCACTACACCAGCCAAAGCCAAGGCCAACGCAGTGAGGCGAGTGATGGCGACCACGT
  CCGCCAGCCTGAAGCGCAGCCCTTCTGCCTCTTCCCTCAGCTCCATGAGCTCAGTGGCCTCCTCTGTGAG
  CAGCAGGCCCAGTCGGACAGGACTATTGACTGAAACCTCCTCCCGTTACGCCAGGAAGATCTCCGGTACC
  ACTGCCCTCCAGGAGGCCCTGAAGGAGAAGCAGCAGCACATTGAGCAGCTGCTGGCGGAACGGGATCTGG
  AGAGGGCGGAGGTGGCCAAGGCCACGAGCCACGTGGGGGAGATAGAGCAGGAGCTAGCTCTGGCCCGGGA
  CGGACATGACCAGCATGTCCTGGAATTGGAAGCCAAAATGGACCAGCTGCGAACAATGGTGGAAGCTGCT
  GACAGGGAGAAGGTGGAGCTTCTCAACCAGCTTGAAGAGGAGAAAAGGAAGGTTGAGGACCTTCAGTTCC
  GGGTTGAAGAAGAATCAATTACCAAAGGTGATCTTGAGGTGGCTACAGTTTCAGAAAAGTCACGTATAAT
  GGAACTGGAGAAAGACCTAGCATTGAGAGTACAGGAAGTAGCTGAGCTCCGAAGAAGGCTAGAGTCCAAT
  AAGCCTGCTGGGGATGTGGACATGTCACTTTCCCTTTTGCAAGAGATAAGCTCTTTGCAAGAAAAGTTAG
  AAGTCACCCGTACTGACCACCAGAGAGAAATAACTTCTCTGAAGGAGCATTTTGGAGCCCGGGAAGAAAC
  TCATCAGAAGGAGATAAAGGCTCTGTATACCGCCACGGAAAAGCTTTCCAAAGAGAACGAGTCATTGAAA
  AGCAAGCTGGAGCATGCCAACAAAGAGAACTCAGATGTGATAGCTCTATGGAAGTCCAAACTGGAGACTG
  CCATCGCATCCCACCAGCAGGCGATGGAAGAACTGAAGGTATCTTTCAGCAAAGGGCTTGGAACAGAGAC
  GGCAGAATTTGCTGAACTAAAAACACAAATAGAGAAAATGAGACTAGATTACCAACACGAAATAGAAAAT
  TTGCAGAATCAACAAGACTCTGAACGGGCTGCCCATGCTAAAGAGATGGAAGCCTTGAGGGCTAAACTGA
  TGAAAGTTATTAAAGAAAAGGAAAACAGTCTGGAAGCCATCAGGTCGAAACTGGACAAAGCAGAAGACCA
  GCATCTCGTAGAAATGGAAGACACGTTAAACAAATTACAGGAAGCTGAAATAAAGGTAAAGGAGCTAGAG
  GTACTGCAAGCCAAATGCAATGAACAAACCAAGGTTATTGATAATTTTACATCACAGCTCAAGGCTACTG
  AAGAAAAGCTCTTGGATCTTGATGCACTTCGGAAAGCCAGTTCCGAAGGTAAATCGGAAATGAAGAAACT
  TAGACAGCAGCTTGAGGCAGCTGAGAAACAGATTAAACATTTAGAGATTGAAAAGAATGCTGAAAGTAGC
  AAGGCTAGTAGCATTACCAGAGAGCTCCAGGGGAGAGAGCTAAAGCTTACTAACCTTCAGGAAAATTTGA
  GTGAAGTCAGTCAAGTGAAAGAGACTTTGGAAAAAGAACTTCAGATTTTGAAAGAAAAGTTTGCTGAAGC
  TTCAGAGGAGGCAGTCTCTGTTCAGAGAAGTATGCAAGAAACTGTAAATAAGTTACACCAAAAGGAGGAA
  CAGTTTAACATGCTGTCTTCTGACTTGGAGAAGCTGAGAGAAAACTTAGCAGATATGGAGGCAAAATTTA
  GAGAGAAAGATGAGAGAGAAGAGCAGCTGATAAAGGCAAAGGAAAAACTGGAAAATGACATTGCAGAAAT
  AATGAAGATGTCAGGAGATAACTCTTCTCAGCTGACAAAAATGAACGATGAATTACGTCTGAAAGAAAGA
  GATGTAGAAGAATTACAGCTAAAACTTACAAAGGCTAATGAAAATGCAAGTTTTCTGCAAAAAAGTATTG
  AGGACATGACTGTCAAAGCTGAACAGAGCCAGCAAGAAGCAGCTAAAAAGCATGAGGAAGAAAAGAAAGA
  ATTGGAGAGGAAATTGTCGGACCTGGAAAAGAAAATGGAAACAAGCCACAACCAGTGTCAGGAGCTGAAA
  GCCAGGTATGAGAGAGCCACTTCTGAGACAAAAACCAAGCATGAAGAAATCCTACAGAACCTCCAGAAGA
  CGCTGCTGGACACAGAGGACAAGCTGAAGGGCGCACGGGAGGAGAACAGTGGCTTGCTGCAGGAGCTGGA
  GGAGCTGAGAAAGCAAGCCGACAAAGCCAAAGCTGCTCAAACAGCGGAAGATGCCATGCAGATAATGGAA
  CAGATGACCAAAGAGAAGACTGAGACTCTGGCCTCCTTGGAGGACACCAAGCAAACAAATGCAAAACTAC
  AGAATGAATTGGACACACTTAAAGAAAACAACTTGAAAAATGTGGAAGAGCTGAACAAATCAAAAGAACT
  CCTGACTGTAGAGAATCAAAAAATGGAAGAATTTAGGAAAGAAATAGAAACCCTAAAGCAGGCAGCAGCT
  CAGAAGTCCCAGCAGCTTTCAGCGTTGCAAGAAGAGAACGTTAAACTTGCTGAGGAGCTGGGGAGAAGCA
  GGGACGAAGTCACAAGTCATCAAAAGCTGGAAGAAGAAAGATCTGTGCTCAATAATCAGTTGTTAGAAAT
  GAAAAAAAGAGAATCCAAGTTCATAAAAGACGCAGATGAAGAGAAAGCTTCCTTGCAGAAATCCATCAGT
  ATAACTAGTGCCTTACTCACAGAAAAGGATGCCGAGCTGGAGAAACTGAGAAATGAGGTCACAGTGCTCA
  GGGGAGAAAACGCCTCTGCCAAGTCCTTGCATTCAGTTGTTCAGACTCTAGAGTCTGATAAGGTGAAGCT
  CGAGCTCAAGGTAAAGAACTTGGAGCTTCAACTCAAAGAAAACAAGAGGCAGCTCAGCAGCTCCTCAGGT
  AATACAGACACTCAGGCAGACGAGGATGAAAGAGCCCAGGAGAGTCAGATTGATTTCCTAAATTCAGTAA
  TAGTGGACCTTCAAAGGAAGAATCAAGACCTCAAGATGAAGGTGGAGATGATGTCAGAAGCAGCCCTGAA
  TGGGAACGGGGATGACCTAAACAATTATGACAGTGATGATCAGGAGAAACAGTCCAAGAAGAAACCTCGC
  CTCTTCTGTGACATTTGTGACTGCTTTGATCTCCACGACACAGAGGATTGTCCTACCCAGGCACAGATGT
  CAGAGGACCCTCCCCATTCCACACACCATGGCAGTCGGGGTGAGGAACGCCCATACTGTGAAATCTGTGA
  GATGTTTGGACACTGGGCCACCAACTGCAATGACGACGAAACCTTCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_198240
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198240.1, NP_937883.1
• RefSeq Size: 5793 bp
• RefSeq ORF: 4179 bp
• Locus ID: 6249
• Cytogenetics: 12q24.31
• Gene Summary: 'The protein encoded by this gene links endocytic vesicles to microtubules. This gene is highly expressed in Reed-Sternberg cells of Hodgkin disease. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]'
  Transcript Variant: This variant (2) lacks two consecutive alternate in-frame exons compared to variant 3. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform c.