RCC1 (NM_001269) Human Untagged Clone

CAT#: SC310410

RCC1 (untagged)-Human regulator of chromosome condensation 1 (RCC1), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RCC1
• Synonyms: CHC1; RCC1-I; SNHG3-RCC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001269, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCACCCAAGCGCATAGCTAAAAGAAGGTCCCCCCCAGCAGATGCCATCCCCAAAAGCAAGAAGGTGA
  AGGTCTCACACAGGTCCCACAGCACAGAACCCGGCTTGGTGCTGACACTAGGCCAGGGCGACGTGGGCCA
  GCTGGGGCTGGGTGAGAATGTGATGGAGAGGAAGAAGCCGGCCCTGGTATCCATTCCGGAGGATGTTGTG
  CAGGCTGAGGCTGGGGGCATGCACACCGTGTGTCTAAGCAAAAGTGGCCAGGTCTATTCCTTCGGCTGCA
  ATGATGAGGGTGCCCTGGGAAGGGACACATCAGTGGAGGGCTCGGAGATGGTCCCTGGGAAAGTGGAGCT
  GCAAGAGAAGGTGGTACAGGTGTCAGCAGGAGACAGTCACACAGCAGCCCTCACCGATGATGGCCGTGTC
  TTCCTCTGGGGCTCCTTCCGGGACAATAACGGTGTGATTGGACTGTTGGAGCCCATGAAGAAGAGCATGG
  TGCCTGTGCAGGTGCAGCTGGATGTGCCTGTGGTAAAGGTGGCCTCAGGAAACGACCACTTGGTGATGCT
  GACAGCTGATGGTGACCTCTACACCTTGGGCTGCGGGGAACAGGGCCAGCTAGGCCGTGTGCCTGAGTTA
  TTTGCCAACCGTGGTGGCCGGCAAGGCCTCGAACGACTCCTGGTCCCCAAGTGTGTGATGCTGAAATCCA
  GGGGAAGCCGGGGCCACGTGAGATTCCAGGATGCCTTTTGTGGTGCCTATTTCACCTTTGCCATCTCCCA
  TGAGGGCCACGTGTACGGCTTCGGCCTCTCCAACTACCATCAGCTTGGAACTCCGGGCACAGAATCTTGC
  TTCATACCCCAGAACCTAACATCCTTCAAGAATTCCACCAAGTCCTGGGTGGGCTTCTCTGGTGGCCAGC
  ACCATACAGTCTGCATGGATTCGGAAGGAAAAGCATACAGCCTGGGCCGGGCTGAGTATGGGCGGCTGGG
  CCTTGGAGAGGGTGCTGAGGAGAAGAGCATACCCACCCTCATCTCCAGGCTGCCTGCTGTCTCCTCGGTG
  GCTTGTGGGGCCTCTGTGGGGTATGCTGTGACCAAGGATGGTCGTGTTTTCGCCTGGGGCATGGGCACCA
  ACTACCAGCTGGGCACAGGGCAGGATGAGGACGCCTGGAGCCCTGTGGAGATGATGGGCAAACAGCTGGA
  GAACCGTGTGGTCTTATCTGTGTCCAGCGGGGGCCAGCATACAGTCTTATTAGTCAAGGACAAAGAACAG
  AGCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001269
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001269.4, NP_001260.1
• RefSeq Size: 2540 bp
• RefSeq ORF: 1266 bp
• Locus ID: 1104
• Cytogenetics: 1p35.3
• Domains: RCC1
• Gene Summary: ''
  Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1, resulting in an isoform (c) that is shorter than isoform a. Both variants 3 and 4 encode the same isoform.