INCENP (NM_001040694) Human Untagged Clone

CAT#: SC311138

INCENP (untagged)-Human inner centromere protein antigens 135/155kDa (INCENP), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: INCENP
• Synonyms: FLJ31633; MGC111393
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001040694, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGGACGACGGCCCCAGGGCCCATTCACCTGCTGGAGCTATGTGACCAGAAGCTCATGGAGTTTCTCT
  GCAACATGGATAATAAGGACTTGGTGTGGCTTGAGGAAATCCAAGAGGAGGCCGAGCGCATGTTCACCAG
  AGAATTCAGCAAAGAGCCAGAGCTGATGCCCAAAACACCTTCTCAGAAGAACCGACGGAAGAAGAGACGG
  ATTTCTTATGTTCAGGATGAAAACAGAGATCCCATCAGGAGAAGGTTATCCCGCAGAAAGTCTCGGAGCA
  GCCAGCTGAGCTCCCGACGCCTCCGCAGCAAGGACAGTGTAGAGAAGCTGGCTACAGTGGTCGGGGAGAA
  CGGCTCCGTCCTGCGGCGTGTGACCCGTGCTGCGGCTGCAGCTGCCGCGGCTACCATGGCATTGGCTGCA
  CCTTCTTCACCCACCCCTGAGTCTCCCACGATGCTGACTAAGAAGCCCGAGGATAACCACACCCAGTGCC
  AGCTGGTGCCTGTGGTGGAGATCGGCATCAGTGAGCGCCAGAATGCTGAGCAGCATGTCACCCAGCTCAT
  GTCCACCGAGCCTCTGCCCCGCACTCTGTCCCCGACTCCAGCTTCAGCCACAGCTCCAACCTCCCAGGGC
  ATCCCGACATCAGATGAGGAATCAACACCTAAGAAGTCGAAGGCCAGGATACTGGAGTCCATCACAGTGA
  GCTCCCTGATGGCTACACCCCAGGACCCCAAGGGTCAAGGGGTCGGGACGGGGCGGTCTGCGTCTAAGCT
  CAGGATTGCGCAGGTCTCCCCTGGCCCACGGGACTCGCCAGCCTTTCCAGATTCTCCATGGCGGGAGCGG
  GTGCTGGCTCCCATCCTGCCGGATAACTTCTCCACGCCCACGGGCTCTCGCACGGACTCTCAATCGGTGC
  GGCACAGCCCGATCGCCCCGTCTTCCCCGAGTCCCCAAGTCTTAGCCCAGAAGTACTCTCTGGTGGCCAA
  ACAGGAAAGTGTTGTCCGCAGGGCGAGCAGAAGGCTTGCCAAGAAGACTGCCGAAGAGCCAGCTGCCTCT
  GGCCGCATCATCTGTCACAGTTACCTGGAGAGGCTCCTGAATGTTGAGGTGCCCCAGAAAGTTGGTTCTG
  AGCAGAAGGAACCCCCCGAGGAGGCTGAGCCTGTGGCGGCAGCTGAGCCAGAGGTCCCTGAGAACAATGG
  AAATAACTCGTGGCCCCACAATGACACGGAGATTGCCAACAGCACACCCAACCCGAAGCCTGCAGCCAGC
  AGCCCGGAAACACCCTCTGCAGGGCAGCAAGAGGCCAAGACGGACCAAGCAGATGGACCCAGAGAGCCAC
  CGCAGAGTGCCAGGAGGAAGCGCAGCTACAAGCAGGCCGTGAGTGAGCTGGACGAGGAGCAGCACCTGGA
  GGATGAGGAGCTGCAGCCCCCCAGGAGCAAGACCCCTTCCTCACCCTGCCCAGCCAGCAAGGTGGTACGG
  CCCCTCCGGACCTTTCTGCACACAGTGCAGAGGAACCAGATGCTCATGACCCCGACCTCAGCCCCACGCA
  GCGTCATGAAGTCCTTTATTAAGCGCAACACTCCCCTGCGCATGGACCCCAAGTGCAGCTTCGTCGAGAA
  GGAGCGGCAGCGCCTGGAGAATCTGCGGCGGAAGGAGGAGGCCGAGCAGCTGCGCAGGCAGAAGGTGGAG
  GAGGACAAGCGGCGGCGGCTGGAGGAGGTGAAGCTGAAGCGTGAGGAACGCCTCCGCAAGGTGCTGCAGG
  CCCGCGAGCGGGTGGAGCAGATGAAGGAGGAGAAGAAGAAGCAGATTGAGCAGAAGTTTGCTCAGATCGA
  CGAGAAGACTGAGAAGGCCAAGGAGGAGCGGCTGGCAGAGGAGAAGGCCAAGAAAAAGGCGGCGGCCAAG
  AAGATGGAGGAGGTGGAAGCACGCAGGAAGCAGGAAGAGGAGGCACGTAGGCTCAGGTGGCTGCAGCAGG
  AGGAGGAAGAGCGGCGGCACCAAGAGCTGCTGCAGAAGAAGAAGGAAGAGGAGCAGGAGCGGCTGCGGAA
  GGCGGCCGAGGCTAAGCGGCTGGCAGAGCAGCGGGAGCAGGAGCGGCGGGAGCAGGAGCGGCGCGAGCAG
  GAGCGGCGCGAGCAGGAGCGGCGGGAGCAGGAGCGGCGCGAGCAGGAGCGACAGCTGGCAGAGCAGGAGC
  GTCGGCGGGAGCAGGAGCGGCTCCAGGCCGAGAGGGAGCTGCAGGAGCGGGAGAAGGCCCTGCGGCTGCA
  GAAGGAGCAGCTGCAGAGGGAACTGGAGGAGAAGAAGAAGAAGGAAGAGCAGCAGCGTCTGGCTGAGCGG
  CAGCTGCAGGAGGAGCAAGAGAAGAAAGCCAAGGAGGCAGCAGGGGCCAGCAAGGCCCTGAATGTGACTG
  TGGACGTGCAGTCTCCAGCTTGTACCTCATATCAGATGACTCCGCAAGGGCACAGGGCCCCTCCCAAGAT
  CAACCCAGATAACTACGGGATGGATCTGAATAGCGACGACTCCACCGATGATGAGGCCCATCCCCGGAAG
  CCCATCCCCACCTGGGCCCGAGGCACCCCGCTCAGCCAGGCTATCATTCACCAGTACTACCACCCACCGA
  ACCTTCTGGAGCTCTTTGGAACCATTCTCCCACTGGACTTGGAGGATATCTTCAAGAAGAGCAAGCCCCG
  CTATCACAAGCGCACCAGCTCTGCTGTCTGGAACTCACCGCCCCTGCAGGGCGCCAGGGTCCCCAGCAGC
  CTGGCCTACAGCCTGAAGAAGCACTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001040694
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001040694.1, NP_001035784.1
• RefSeq Size: 4146 bp
• RefSeq ORF: 2757 bp
• Locus ID: 3619
• Cytogenetics: 11q12.3
• Protein Families: Druggable Genome
• Gene Summary: 'In mammalian cells, 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger,' or transiently interacting, proteins (reviewed by Choo, 1997). The constitutive proteins include CENPA (centromere protein A; MIM 117139), CENPB (MIM 117140), CENPC1 (MIM 117141), and CENPD (MIM 117142). The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay, 1994 [PubMed 8088460]). These include CENPE (MIM 117143); MCAK (MIM 604538); KID (MIM 603213); cytoplasmic dynein (e.g., MIM 600112); CliPs (e.g., MIM 179838); and CENPF/mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw and Cooke, 1991 [PubMed 1860899]), the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis (Cutts et al., 1999 [PubMed 10369859]).[supplied by OMIM, Mar 2008]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).