Dystrobrevin beta (DTNB) (NM_033147) Human Untagged Clone

CAT#: SC314147

DTNB (untagged)-Human dystrobrevin, beta (DTNB), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DTNB
• Synonyms: MGC17163; MGC57126
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_033147, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGATTGAGGAAAGTGGGAACAAGCGGAAGACCATGGCAGAGAAGAGGCAGCTGTTCATAGAAATGCGTG
  CTCAGAATTTTGATGTCATACGACTATCAACTTACAGAACAGCCTGCAAATTACGATTTGTACAAAAACG
  ATGCAACCTTCATCTTGTTGATATCTGGAACATGATTGAAGCCTTCCGAGACAATGGCCTTAATACACTG
  GACCATACCACCGAGATCAGTGTGTCCCGCCTCGAAACTGTCATCTCCTCCATCTACTATCAGTTGAACA
  AGCGCCTTCCTTCTACTCACCAAATTAGTGTGGAACAATCTATCAGCCTCCTCCTCAACTTTATGATTGC
  TGCATATGACAGTGAGGGCCGAGGCAAGTTGACGGTATTTTCAGTTAAAGCTATGTTAGCAACCATGTGT
  GGTGGAAAAATGCTGGACAAATTGAGATATGTTTTCTCCCAGATGTCAGATTCCAATGGCTTAATGATAT
  TTAGCAAGTTTGACCAGTTTCTGAAGGAAGTTCTGAAGCTCCCAACAGCTGTCTTTGAAGGGCCATCTTT
  TGGTTACACAGAGCACTCAGTCCGCACCTGTTTTCCACAGCAGAGAAAGATAATGCTAAATATGTTTTTA
  GACACAATGATGGCTGACCCTCCTCCCCAGTGCCTTGTCTGGCTACCTCTCATGCACAGGCTTGCCCATG
  TTGAGAATGTCTTCCATCCCGTGGAGTGCTCCTACTGCCGATGTGAGAGTATGATGGGTTTCCGGTACCG
  ATGCCAGCAGTGCCACAACTATCAGCTCTGCCAGAATTGCTTTTGGCGTGGCCATGCCGGCGGCCCTCAC
  AGCAACCAGCACCAGATGAAGGAGCATTCCTCTTGGAAATCTCCTGCAAAGAAGCTGAGCCATGCAATTA
  GTAAATCTTTGGGGTGTGTACCCACGAGAGAACCCCCGCATCCTGTTTTTCCTGAGCAACCAGAGAAACC
  ACTTGACCTTGCACATATAGTTCCTCCTCGCCCTCTGACTAATATGAATGACACCATGGTTAGCCACATG
  TCCTCTGGAGTGCCCACTCCCACCAAGAGGTTACAGTATAGCCAGGATATACCCAGTCACTTGGCCGATG
  AGCATGCGCTGATAGCCTCCTATGTGGCCCGTCTGCAGCACTGTGCACGTGTTCTGGACAGTCCTAGCCG
  ACTGGATGAGGAACACCGTCTTATAGCTCGCTATGCTGCCCGGCTGGCTGCAGAAGCAGGAAACGTGACT
  CGTCCTCCCACTGACTTGAGCTTTAACTTTGATGCCAACAAACAACAAAGACAGCTTATTGCAGAACTGG
  AAAACAAAAACAGAGAGATCCTGCAGGAGATTCAGCGTCTCCGCCTGGAACACGAGCAGGCCTCCCAGCC
  CACCCCTGAGAAGGCACAGCAGAACCCCACGCTGCTGGCAGAGCTGCGGCTGCTGAGGCAAAGGAAGGAT
  GAACTGGAGCAGAGGATGTCGGCCCTGCAGGAGAGCAGGCGGGAGCTGATGGTCCAGCTGGAAGAGCTGA
  TGAAGTTGCTGAAGGAGGAAGAGCAAAAGCAGGCAGCTCAGGCCACAGGGTCACCACATACATCGCCCAC
  CCATGGAGGCGGCCGGCCAATGCCCATGCCAGTGCGCTCCACGTCTGCCGGCTCCACCCCCACCCACTGT
  CCGCAGGACTCGCTGAGCGGAGTCGGGGGAGACGTGCAGGAGGCCTTCGCACAAGCAGAGGAAGGTGCAG
  AGGAAGAAGAAGAGAAGATGCAGAATGGGAAAGACAGAGGTTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_033147
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033147.3, NP_149159.2
• RefSeq Size: 2439 bp
• RefSeq ORF: 1794 bp
• Locus ID: 1838
• Cytogenetics: 2p23.3
• Domains: ZnF_ZZ
• Gene Summary: 'This gene encodes dystrobrevin beta, a component of the dystrophin-associated protein complex (DPC). The DPC consists of dystrophin and several integral and peripheral membrane proteins, including dystroglycans, sarcoglycans, syntrophins and dystrobrevin alpha and beta. The DPC localizes to the sarcolemma and its disruption is associated with various forms of muscular dystrophy. Dystrobrevin beta is thought to interact with syntrophin and the DP71 short form of dystrophin. [provided by RefSeq, Mar 2016]'