DCTN1 (NM_023019) Human Untagged Clone

CAT#: SC315223

DCTN1 (untagged)-Human dynactin 1 (DCTN1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DCTN1
• Synonyms: DAP-150; DP-150; P135
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_023019, the custom clone sequence may differ by one or more nucleotides
  ATGATGAGACAGGCACCGACAGCCCGAAAGACCACAACTCGGCGACCCAAGCCCACGCGC
  CCAGCCAGTACTGGGGTGGCTGGGGCCAGTAGCTCCCTGGGCCCCTCTGGCTCAGCGTCA
  GCAGGTGAGCTGAGCAGCAGTGAGCCCAGCACCCCGGCTCAGACTCCGCTGGCAGCACCC
  ATCATCCCCACGCCGGTCCTCACCTCTCCTGGAGCAGTCCCCCCGCTTCCTTCCCCATCC
  AAGGAGGAGGAGGGACTAAGGGCTCAGGTGCGGGACCTGGAGGAGAAACTAGAGACCCTG
  AGACTGAAACGGGCAGAAGACAAAGCAAAGCTAAAAGAGCTGGAGAAACACAAAATCCAG
  CTGGAGCAGGTGCAGGAATGGAAGAGCAAAATGCAGGAGCAGCAGGCCGACCTGCAGCGG
  CGCCTCAAGGAGGCGAGAAAGGAAGCCAAGGAGGCGCTGGAGGCAAAGGAACGCTATATG
  GAGGAGATGGCTGATACTGCTGATGCCATTGAGATGGCCACTTTGGACAAGGAGATGGCT
  GAAGAGCGGGCTGAGTCCCTGCAGCAGGAGGTGGAGGCACTGAAGGAGCGGGTGGACGAG
  CTCACTACTGACTTAGAGATCCTCAAGGCTGAGATTGAAGAGAAGGGCTCAGATGGCGCT
  GCATCCAGTTATCAGCTCAAGCAGCTTGAGGAGCAGAATGCCCGCCTGAAGGATGCCCTG
  GTGAGGATGCGGGATCTTTCTTCCTCAGAGAAGCAGGAGCATGTGAAGCTCCAGAAGCTC
  ATGGAAAAGAAGAACCAAGAGCTGGAAGTTGTGAGGCAACAGCGGGAGCGTCTGCAGGAG
  GAGCTAAGCCAGGCAGAGAGCACCATTGATGAGCTCAAGGAGCAGGTGGATGCTGCTCTG
  GGTGCTGAGGAGATGGTGGAGATGCTGACAGATCGGAACCTGAATCTGGAAGAGAAAGTG
  CGCGAGTTGAGGGAGACTGTGGGAGACTTGGAAGCGATGAATGAGATGAACGATGAGCTG
  CAGGAGAATGCACGTGAGACAGAACTGGAGCTGCGGGAGCAGCTGGACATGGCAGGCGCG
  CGGGTTCGTGAGGCCCAGAAGCGTGTGGAGGCAGCCCAGGAGACGGTTGCAGACTACCAG
  CAGACCATCAAGAAGTACCGCCAGCTGACCGCCCATCTACAGGATGTGAATCGGGAACTG
  ACAAACCAGCAGGAAGCATCTGTGGAGAGGCAACAGCAGCCACCTCCAGAGACCTTTGAC
  TTCAAAATCAAGTTTGCTGAGACTAAGGCCCATGCCAAGGCAATTGAGATGGAATTGAGG
  CAGATGGAGGTGGCCCAGGCCAATCGACACATGTCCCTGCTGACAGCCTTCATGCCTGAC
  AGCTTCCTTCGGCCAGGTGGGGACCATGACTGCGTTCTGGTGCTGTTGCTCATGCCTCGT
  CTCATTTGCAAGGCAGAGCTGATCCGGAAGCAGGCCCAGGAGAAGTTTGAACTAAGTGAG
  AACTGTTCAGAGCGGCCTGGGCTGCGAGGAGCTGCTGGGGAGCAACTCAGCTTTGCTGCT
  GGACTGGTGTACTCGCTGAGCCTGCTGCAGGCCACGCTACACCGCTATGAGCATGCCCTC
  TCTCAGTGCAGTGTGGATGTGTATAAGAAAGTGGGCAGCCTGTACCCTGAGATGAGTGCC
  CATGAGCGCTCCTTGGATTTCCTCATTGAACTGCTGCACAAGGATCAGCTGGATGAGACT
  GTCAATGTGGAGCCTCTCACCAAGGCCATCAAGTACTATCAGCATCTGTACAGCATCCAC
  CTTGCCGAACAGCCTGAGGACTGTACTATGCAGCTGGCTGACCACATTAAGTTCACGCAG
  AGTGCTCTGGACTGCATGAGTGTGGAGGTAGGACGGCTGCGTGCCTTCTTGCAGGGTGGG
  CAGGAGGCTACAGATATTGCCCTCCTGCTCCGGGATCTGGAAACTTCATGCAGTGACATC
  CGCCAGTTCTGCAAGAAGATCCGAAGGCGAATGCCAGGGACAGATGCTCCTGGGATCCCA
  GCTGCACTGGCCTTTGGACCACAGGTATCTGACACGCTCCTAGACTGCAGGAAACACTTG
  ACGTGGGTCGTGGCTGTGCTGCAGGAGGTGGCAGCTGCTGCTGCCCAGCTCATTGCCCCA
  CTGGCAGAGAATGAGGGGCTACTTGTGGCTGCTCTGGAGGAACTGGCTTTCAAAGCAAGC
  GAGCAGATCTATGGGACCCCCTCCAGCAGCCCCTATGAGTGTCTGCGCCAGTCATGCAAC
  ATCCTCATCAGTACCATGAACAAGCTGGCCACAGCCATGCAGGAGGGGGAGTATGATGCA
  GAGCGGCCCCCCAGCAAGCCTCCACCGGTTGAACTGCGGGCTGCTGCCCTTCGTGCAGAG
  ATCACAGATGCTGAAGGCCTGGGTTTGAAGCTCGAAGATCGAGAGACAGTTATTAAGGAG
  TTGAAGAAGTCACTCAAGATTAAGGGAGAGGAGCTAAGTGAGGCCAATGTGCGGCTGAGC
  CTCCTGGAGAAGAAGTTGGACAGTGCTGCCAAGGATGCAGATGAGCGCATCGAGAAAGTC
  CAGACTCGGCTGGAGGAGACCCAGGCACTGCTGCGAAAGAAGGAGAAAGAGTTTGAGGAG
  ACAATGGATGCACTCCAGGCTGACATCGACCAGCTGGAGGCAGAGAAGGCAGAACTAAAG
  CAGCGTCTGAACAGCCAGTCCAAACGCACGATTGAGGGACTCCGGGGCCCTCCTCCTTCA
  GGCATTGCTACTCTGGTCTCTGGCATTGCTGGTGAAGAACAGCAGCGAGGAGCCATCCCT
  GGGCAGGCTCCAGGGTCTGTGCCAGGCCCAGGGCTGGTGAAGGACTCACCACTGCTGCTT
  CAGCAGATCTCTGCCATGAGGCTGCACATCTCCCAGCTCCAGCATGAGAACAGCATCCTC
  AAGGGAGCCCAGATGAAGGCATCCTTGGCATCCCTGCCCCCTCTGCATGTTGCAAAGCTA
  TCCCATGAGGGCCCTGGCAGTGAGTTACCAGCTGGAGCGCTGTATCGTAAGACCAGCCAG
  CTGCTGGAGACATTGAATCAATTGAGCACACACACGCACGTAGTAGACATCACTCGCACC
  AGCCCTGCTGCCAAGAGCCCGTCGGCCCAACTTATGGAGCAAGTGGCTCAGCTTAAGTCC
  CTGAGTGACACCGTCGAGAAGCTCAAGGATGAGGTCCTCAAGGAGACAGTATCTCAGCGC
  CCTGGAGCCACAGTACCCACTGACTTTGCCACCTTCCCTTCATCAGCCTTCCTCAGGGCC
  AAGGAGGAGCAGCAGGATGACACAGTCTACATGGGCAAAGTGACCTTCTCATGTGCGGCT
  GGTTTTGGACAGCGACACCGGCTGGTGCTGACCCAGGAGCAGCTGCACCAGCTTCACAGT
  CGCCTCATCTCC
  
• Restriction Sites: Please inquire
• ACCN: NM_023019
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023019.1, NP_075408.1
• RefSeq Size: 3790 bp
• RefSeq ORF: 3435 bp
• Locus ID: 1639
• Cytogenetics: 2p13.1
• Domains: M
• Protein Families: Druggable Genome
• Protein Pathways: Huntington's disease
• Gene Summary: 'This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA). [provided by RefSeq, Oct 2008]'
  Transcript Variant: This variant (2) contains an additional in-frame exon in the 3' end, compared to variant 4, resulting in a protein (isoform 2) with a longer C-terminus, compared to isoform 4. Isoform 2 is also called p135. CCDS Note: This CCDS representation lacks publicly available full-length transcript support. Its exon combination is based on mRNA annotation on DNA accession AF064205.1 (AAD55812.1 product) from PMID:9799602.