RCC1 (NM_001048195) Human Untagged Clone

CAT#: SC315432

RCC1 (untagged)-Human regulator of chromosome condensation 1 (RCC1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RCC1
• Synonyms: CHC1; RCC1-I; SNHG3-RCC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001048195, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCACCCAAGCGCATAGCTAAAAGAAGGTCCCCCCCAGCAGATGCCATCCCCAAAAGCAAGAAGGTGA
  AGGACACGAGGGCCGCTGCCTCCCGCCGCGTTCCTGGCGCCCGCTCCTGCCAAGTCTCACACAGGTCCCA
  CAGCACAGAACCCGGCTTGGTGCTGACACTAGGCCAGGGCGACGTGGGCCAGCTGGGGCTGGGTGAGAAT
  GTGATGGAGAGGAAGAAGCCGGCCCTGGTATCCATTCCGGAGGATGTTGTGCAGGCTGAGGCTGGGGGCA
  TGCACACCGTGTGTCTAAGCAAAAGTGGCCAGGTCTATTCCTTCGGCTGCAATGATGAGGGTGCCCTGGG
  AAGGGACACATCAGTGGAGGGCTCGGAGATGGTCCCTGGGAAAGTGGAGCTGCAAGAGAAGGTGGTACAG
  GTGTCAGCAGGAGACAGTCACACAGCAGCCCTCACCGATGATGGCCGTGTCTTCCTCTGGGGCTCCTTCC
  GGGACAATAACGGTGTGATTGGACTGTTGGAGCCCATGAAGAAGAGCATGGTGCCTGTGCAGGTGCAGCT
  GGATGTGCCTGTGGTAAAGGTGGCCTCAGGAAACGACCACTTGGTGATGCTGACAGCTGATGGTGACCTC
  TACACCTTGGGCTGCGGGGAACAGGGCCAGCTAGGCCGTGTGCCTGAGTTATTTGCCAACCGTGGTGGCC
  GGCAAGGCCTCGAACGACTCCTGGTCCCCAAGTGTGTGATGCTGAAATCCAGGGGAAGCCGGGGCCACGT
  GAGATTCCAGGATGCCTTTTGTGGTGCCTATTTCACCTTTGCCATCTCCCATGAGGGCCACGTGTACGGC
  TTCGGCCTCTCCAACTACCATCAGCTTGGAACTCCGGGCACAGAATCTTGCTTCATACCCCAGAACCTAA
  CATCCTTCAAGAATTCCACCAAGTCCTGGGTGGGCTTCTCTGGTGGCCAGCACCATACAGTCTGCATGGA
  TTCGGAAGGAAAAGCATACAGCCTGGGCCGGGCTGAGTATGGGCGGCTGGGCCTTGGAGAGGGTGCTGAG
  GAGAAGAGCATACCCACCCTCATCTCCAGGCTGCCTGCTGTCTCCTCGGTGGCTTGTGGGGCCTCTGTGG
  GGTATGCTGTGACCAAGGATGGTCGTGTTTTCGCCTGGGGCATGGGCACCAACTACCAGCTGGGCACAGG
  GCAGGATGAGGACGCCTGGAGCCCTGTGGAGATGATGGGCAAACAGCTGGAGAACCGTGTGGTCTTATCT
  GTGTCCAGCGGGGGCCAGCATACAGTCTTATTAGTCAAGGACAAAGAACAGAGCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001048195
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001048195.2, NP_001041660.1
• RefSeq Size: 2591 bp
• RefSeq ORF: 1317 bp
• Locus ID: 1104
• Cytogenetics: 1p35.3
• Gene Summary: ''
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a.