DOCK1 (NM_001380) Human Untagged Clone

CAT#: SC315655

DOCK1 (untagged)-Human dedicator of cytokinesis 1 (DOCK1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DOCK1
• Synonyms: ced5; DOCK180
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001380 edited
  ATGGAAAATGGCGGCCTAGACGCGGAGTTTCCTGCCCGACCCGCGGCGGCTCCGGCGGCG
  CCATGACGCGCTGGGTGCCCACCAAGCGCGAGGAGAAGTACGGCGTGGCTTTTTATAACT
  ATGATGCCAGAGGAGCGGATGAACTTTCTTTACAGATCGGAGACACTGTGCACATCTTAG
  AAACATATGAAGGGTGGTACCGAGGTTACACGTTACGAAAAAAGTCTAAGAAGGGTATAT
  TTCCTGCTTCATATATTCATCTTAAAGAAGCGATAGTTGAAGGAAAAGGGCAACATGAAA
  CAGTCATCCCGGGTGACCTCCCCCTCATCCAGGAAGTCACCACGACACTCCGAGAGTGGT
  CCACCATCTGGAGGCAGCTCTACGTGCAAGATAACAGGGAGATGTTTCGAAGTGTGCGGC
  ACATGATCTATGACCTTATTGAATGGCGATCACAAATTCTTTCTGGAACTCTGCCTCAGG
  ATGAACTCAAAGAACTGAAGAAGAAGGTCACAGCCAAAATTGATTATGGAAACAGAATTC
  TAGATTTGGACCTGGTGGTTAGAGATGAAGATGGGAATATTTTGGATCCAGAATTAACTA
  GCACGATTAGTCTCTTCAGAGCTCATGAAATAGCTTCTAAACAAGTGGAGGAAAGGTTAC
  AAGAGGAAAAATCTCAAAAGCAGAACATAGATATTAACAGACAAGCCAAGTTTGCTGCAA
  CCCCTTCTCTGGCCTTGTTTGTGAACCTCAAAAATGTGGTTTGTAAAATAGGAGAAGATG
  CTGAAGTCCTCATGTCTCTATATGACCCTGTGGAGTCCAAATTCATCAGTGAGAACTACC
  TGGTTCGCTGGTCCAGTTCAGGATTACCTAAAGACATAGACAGATTACATAATTTGCGAG
  CCGTGTTTACTGACCTCGGAAGCAAAGACCTGAAAAGGGAGAAAATCAGTTTTGTCTGTC
  AGATTGTTCGCGTGGGTCGCATGGAGCTGAGGGACAACAACACCAGGAAACTGACCTCGG
  GGTTGCGGCGACCTTTTGGAGTGGCTGTGATGGATGTAACAGATATAATAAATGGAAAAG
  TAGATGATGAAGATAAGCAGCATTTCATTCCCTTTCAGCCGGTGGCAGGGGAGAATGACT
  TCCTTCAGACTGTTATAAACAAAGTCATCGCTGCCAAAGAAGTCAACCACAAGGGGCAGG
  GTTTGTGGGTAACATTGAAATTACTTCCTGGAGATATCCATCAGATCCGAAAAGAGTTTC
  CGCATTTAGTGGACAGGACCACAGCTGTGGCTCGAAAAACAGGGTTTCCGGAGATAATCA
  TGCCTGGTGATGTTCGAAATGATATCTATGTAACATTAGTTCAAGGAGATTTTGATAAAG
  GAAGCAAAACAACAGCGAAGAACGTGGAGGTCACGGTGTCTGTGTACGATGAGGATGGGA
  AACGATTAGAGCATGTGATTTTCCCGGGTGCTGGTGATGAAGCGATTTCAGAGTACAAAT
  CTGTGATTTACTACCAAGTAAAGCAGCCACGCTGGTTTGAGACTGTTAAGGTGGCCATTC
  CCATCGAGGACGTTAACCGCAGTCACCTTCGGTTTACCTTCCGCCACAGGTCATCACAGG
  ACTCTAAGGATAAATCTGAGAAAATATTTGCACTAGCATTTGTCAAGCTGATGAGATACG
  ATGGTACCACCCTGCGAGACGGAGAGCACGATCTTATCGTCTATAAGGCCGAAGCGAAGA
  AGCTGGAAGATGCTGCCACGTACTTGAGTCTGCCCTCCACGAAGGCAGAGTTGGAAGAAA
  AGGGCCACTCGGCCACCGGCAAGAGCATGCAGAGCCTTGGGAGCTGCACCATTAGCAAGG
  ACTCCTTCCAGATCTCCACGCTCGTGTGCTCCACCAAACTGACTCAGAACGTGGACCTTC
  TGGGGCTCTTGAAATGGCGCTCCAACACCAGCCTGCTGCAGCAGAACTTGAGGCAGCTGA
  TGAAAGTCGATGGTGGTGAAGTAGTGAAGTTTCTTCAGGACACGTTGGATGCCCTCTTCA
  ACATCATGATGGAGAACTCAGAGAGTGAGACTTTTGACACGTTAGTCTTTGATGCTCTGG
  TATTTATCATTGGACTGATTGCTGATAGAAAATTTCAGCATTTTAATCCTGTTTTGGAAA
  CTTACATTAAGAAACACTTTAGTGCAACGTTAGCCTACACGAAGTTGACAAAAGTGTTGA
  AGAACTACGTGGACGGTGCTGAGAAGCCGGGAGTAAATGAGCAGCTGTACAAAGCCATGA
  AAGCGCTAGAATCCATCTTCAAGTTCATCGTGCGCTCCAGGATCCTGTTCAATCAACTGT
  ATGAAAACAAGGGAGAGGCTGACTTCGTGGAATCTTTGCTGCAGCTCTTCAGGTCCATCA
  ATGACATGATGAGCAGCATGTCAGACCAGACCGTCCGGGTGAAGGGGGCAGCACTGAAAT
  ACTTACCAACGATCGTCAACGATGTGAAATTGGTGTTTGATCCCAAAGAGCTCAGCAAAA
  TGTTTACTGAATTCATCCTCAATGTTCCCATGGGCTTGCTGACCATCCAGAAACTCTACT
  GCTTGATCGAAATCGTCCACAGTGACCTCTTCACACAGCATGACTGCAGAGAGATCCTGC
  TTCCCATGATGACCGATCAGCTCAAGTACCATCTGGAGAGACAGGAGGACCTGGAGGCCT
  GCTGTCAGCTGCTCAGCCACATCCTGGAGGTGCTGTACAGGAAGGACGTGGGGCCAACCC
  AGAGGCACGTCCAGATTATCATGGAGAAACTTCTCCGGACCGTGAACCGAACCGTCATTT
  CCATGGGACGGGATTCTGAACTCATTGGAAACTTCGTGGCTTGCATGACAGCTATTTTAC
  GACAAATGGAAGATTACCATTATGCCCACTTGATCAAGACTTTTGGGAAAATGAGGACTG
  ATGTGGTAGATTTCCTAATGGAAACATTCATCATGTTTAAGAACCTCATTGGAAAGAACG
  TTTACCCCTTCGACTGGGTGATCATGAACATGGTGCAAAATAAAGTCTTCCTGCGAGCAA
  TTAATCAGTATGCAGATATGCTGAACAAAAAATTTCTGGATCAAGCCAACTTTGAGCTAC
  AGCTGTGGAACAACTACTTTCACCTGGCTGTTGCTTTCCTTACTCAAGAGTCCCTGCAAC
  TGGAGAATTTTTCAAGTGCCAAGAGAGCCAAAATCCTTAACAAGTACGGAGATATGAGGA
  GACAGATTGGCTTTGAAATCAGAGACATGTGGTACAACCTTGGTCAACACAAGATAAAGT
  TCATTCCAGAAATGGTGGGCCCAATATTAGAAATGACATTAATTCCCGAGACGGAGCTGC
  GCAAAGCCACCATCCCCATCTTCTTTGATATGATGCAGTGTGAATTCCATTCGACCCGAA
  GCTTCCAAATGTTTGAAAATGAGATCATCACCAAGCTGGATCATGAAGTCGAAGGAGGCA
  GAGGAGACGAACAGTACAAAGTGTTATTTGATAAAATCCTTCTGGAACACTGCAGGAAGC
  ACAAATACCTCGCCAAAACAGGAGAAACTTTTGTAAAACTCGTTGTGCGCTTAATGGAAA
  GGCTTTTGGATTATAGAACCATCATGCACGACGAGAACAAAGAAAACCGCATGAGCTGCA
  CCGTCAATGTGCTGGATTTCTACAAAGAAATTGAAAGAGAAGAAATGTATATAAGGTATT
  TGTACAAGCTCTGTGACCTGCACAAGGAGTGTGATAACTACACCGAAGCGGCTTACACCT
  TGCTTCTCCATGCAAAGCTTCTTAAGTGGTCGGAGGATGTGTGTGTGGCCCACCTCACCC
  AGCGGGACGGGTACCAGGCCACCACGCAGGGACAGCTGAAGGAGCAGCTCTACCAGGAAA
  TCATCCACTACTTCGACAAAGGCAAGATGTGGGAGGAGGCCATTGCCTTGGGCAAGGAGC
  TAGCCGAGCAGTATGAGAACGAAATGTTTGATTATGAGCAACTCAGCGAATTGCTGAAAA
  AACAGGCTCAGTTTTATGAAAACATCGTCAAAGTGATCAGGCCCAAGCCTGACTATTTTG
  CTGTTGGCTACTACGGACAAGGGTTCCCCACATTCCTGCGGGGAAAAGTTTTCATTTACC
  GAGGGAAAGAGTATGAGCGCCGGGAAGATTTTGAGGCTCGGCTCTTAACTCAGTTTCCAA
  ACGCCGAGAAAATGAAGACAACATCTCCACCAGGCGACGATATTAAAAACTCTCCTGGCC
  AGTATATTCAGTGCTTCACAGTGAAGCCCAAACTCGATCTGCCTCCTAAGTTTCACAGGC
  CAGTGTCAGAGCAGATTGTAAGTTTTTACAGGGTGAACGAGGTCCAGCGATTTGAATATT
  CTCGGCCAATCCGGAAGGGAGAGAAAAACCCAGACAATGAATTTGCGAATATGTGGATCG
  AGAGAACCATATATACAACTGCATATAAATTACCTGGAATTTTAAGGTGGTTTGAGGTCA
  AGTCTGTTTTCATGGTGGAAATCAGCCCCCTGGAGAATGCCATTGAGACCATGCAGCTGA
  CGAACGACAAGATCAACAGCATGGTGCAGCAGCACCTGGATGACCCCAGCCTGCCCATCA
  ACCCGCTCTCCATGCTCCTGAACGGCATCGTGGACCCAGCTGTCATGGGGGGCTTCGCAA
  ACTACGAAAAGGCCTTCTTTACAGACCGGTACCTGCAGGAGCACCCTGAGGCCCATGAAA
  AGATCGAGAAGCTCAAGGACCTGATTGCTTGGCAGATTCCTTTTCTGGCCGAAGGGATCA
  GAATCCATGGAGACAAAGTCACGGAGGCACTGAGGCCGTTCCACGAGAGGATGGAGGCCT
  GTTTCAAACAGCTGAAGGAAAAGGTGGAGAAAGAGTACGGCGTCCGAATCATGCCCTCAA
  GTCTGGATGATAGAAGAGGCAGCCGCCCCCGGTCCATGGTGCGGTCCTTCACGATGCCTT
  CCTCATCCCGCCCTCTGTCTGTGGCCTCTGTCTCTTCCCTCTCATCGGACAGCACCCCTT
  CCAGACCAGGCTCCGACGGGTTTGCCCTGGAGCCTCTCCTGCCAAAGAAAATGCACTCCA
  GGTCCCAGGACAAGCTGGACAAGGATGACCTGGAGAAGGAGAAGAAGGACAAGAAGAAGG
  AAAAAAGGAACAGCAAACATCAAGAGATATTTGAGAAAGAATTTAAACCCACCGACATTT
  CCCTGCAGCAGTCTGAGGCTGTGATCCTTTCGGAAACGATAAGTCCCCTGCGGCCCCAGA
  GACCGAAGAGCCAGGTGATGAACGTCATTGGAAGCGAAAGGCGCTTCTCGGTGTCCCCCT
  CGTCACCGTCCTCCCAGCAAACACCCCCTCCAGTTACACCAAGAGCCAAGCTCAGCTTCA
  GCATGCAGTCGAGCTTGGAGCTGAACGGCATGACGGGGGCGGACGTGGCCGATGTCCCAC
  CCCCTCTGCCTCTCAAAGGCAGCGTGGCAGATTACGGGAATTTGATGGAAAACCAGGACT
  TGCTGGGCTCGCCAACACCTCCACCTCCCCCTCCACACCAGAGGCATCTGCCACCTCCAC
  TGCCCAGCAAAACTCCGCCTCCTCCCCCTCCAAAGACAACTCGCAAGCAGACATCGGTGG
  ACTCCGGGATCGTGCAGTGACGTCGCAAGGCTCTCTGGAAAGAGTGTGCTGCCCCTCC
  
• Restriction Sites: Please inquire
• ACCN: NM_001380
• Insert Size: 5700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone has been fully sequenced and found to contain one SNP compared with NM_001380.3.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001380.3, NP_001371.1
• RefSeq Size: 6751 bp
• RefSeq ORF: 5598 bp
• Locus ID: 1793
• Cytogenetics: 10q26.2
• Domains: SH3
• Protein Families: Druggable Genome
• Protein Pathways: Focal adhesion, Regulation of actin cytoskeleton
• Gene Summary: 'This gene encodes a member of the dedicator of cytokinesis protein family. Dedicator of cytokinesis proteins act as guanine nucleotide exchange factors for small Rho family G proteins. The encoded protein regulates the small GTPase Rac, thereby influencing several biological processes, including phagocytosis and cell migration. Overexpression of this gene has also been associated with certain cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]'
  Transcript Variant: This variant (2) uses an alternate splice site at an internal exon, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1.