GPR34 (NM_001097579) Human Untagged Clone

CAT#: SC316196

GPR34 (untagged)-Human G protein-coupled receptor 34 (GPR34), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GPR34
• Synonyms: LYPSR1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001097579, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGAAGTCATACCATAACAATGACGACAACTTCAGTCAGCAGCTGGCCTTACTCCTCCCACAGAATGC
  GCTTTATAACCAATCATAGCGACCAACCGCCACAAAACTTCTCAGCAACACCAAATGTTACTACCTGTCC
  CATGGATGAAAAATTGCTATCTACTGTGTTAACCACATCCTACTCTGTTATTTTCATCGTGGGACTGGTT
  GGGAACATAATCGCCCTCTATGTATTTCTGGGTATTCACCGTAAAAGAAATTCCATTCAAATTTATCTAC
  TTAACGTAGCCATTGCAGACCTCCTACTCATCTTCTGCCTCCCTTTCCGAATAATGTATCATATTAACCA
  AAACAAGTGGACACTAGGTGTGATTCTGTGCAAGGTTGTGGGAACACTGTTTTATATGAACATGTACATT
  AGCATTATTTTGCTTGGATTCATCAGTTTGGATCGCTATATAAAAATTAATCGGTCTATACAGCAACGGA
  AGGCAATAACAACCAAACAAAGTATTTATGTCTGTTGTATAGTATGGATGCTTGCTCTTGGTGGATTCCT
  AACTATGATTATTTTAACACTTAAGAAAGGAGGGCATAATTCCACAATGTGTTTCCATTACAGAGATAAG
  CATAACGCAAAAGGAGAAGCCATTTTTAACTTCATTCTTGTGGTAATGTTCTGGCTAATTTTCTTACTAA
  TAATCCTTTCATATATTAAGATTGGGAAGAATCTATTGAGGATTTCTAAAAGGAGGTCAAAATTTCCTAA
  TTCTGGTAAATATGCCACTACAGCTCGTAACTCCTTTATTGTACTTATCATTTTTACTATATGTTTTGTT
  CCCTATCATGCCTTTCGATTCATCTACATTTCTTCACAGCTAAATGTATCATCTTGCTACTGGAAAGAAA
  TTGTTCACAAAACCAATGAGATCATGCTGGTTCTCTCATCTTTCAATAGTTGCTTAGATCCAGTCATGTA
  TTTCCTGATGTCCAGTAACATTCGCAAAATAATGTGCCAACTTCTTTTTAGACGATTTCAAGGTGAACCA
  AGTAGGAGTGAAAGCACTTCAGAATTTAAACCAGGATACTCCCTGCATGATACATCTGTGGCAGTGAAAA
  TACAGTCTAGTTCTAAAAGTACTTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001097579
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001097579.1, NP_001091048.1
• RefSeq Size: 1938 bp
• RefSeq ORF: 1146 bp
• Locus ID: 2857
• Cytogenetics: Xp11.4
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Gene Summary: 'G protein-coupled receptors (GPCRs), such as GPR34, are integral membrane proteins containing 7 putative transmembrane domains (TMs). These proteins mediate signals to the interior of the cell via activation of heterotrimeric G proteins that in turn activate various effector proteins, ultimately resulting in a physiologic response.[supplied by OMIM, Apr 2006]'
  Transcript Variant: This variant (4) represents the longer transcript. Both variants 1 and 4 encode the same protein.