Calpastatin (CAST) (NM_001750) Human Untagged Clone

CAT#: SC318276

CAST (untagged)-Human calpastatin (CAST), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CAST
• Synonyms: BS-17; PLACK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001750, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCCCAGCCCGGCCAGAAGCCCGCCGCCTCCCCGCGGCCCCGGCGAGCAGCCGCCGCCCGCCGCACCC
  ATGAGCATGTCAGTGAAAAAACCAGTGAATCGCCTTCCAAACCAGGAGAAAAGAAAGGATCAGATGAGAA
  AAAAGCAGCAAGCCTCGGCAGCAGTCAATCCTCCAGAACCTATGCTGGTGGAACAGCCTCGGCCACCAAG
  GTGTCAGCTTCCTCTGGTGCAACCAGCAAGTCTTCCAGTATGAATCCCACAGAAACCAAGGCCATTCCAG
  TCAGCCAACAGATGGAAGGACCACATCTTCCTAACAAGAAAAAACACAAAAAACAGGCTGTAAAAACAGA
  ACCTGAGAAGAAGTCACAGTCAACCAAGCTGTCTGTGGTTCATGAGAAAAAATCCCAAGAAGGAAAGCCA
  AAAGAACACACAGAGCCAAAAAGCCTACCCAAGCAGGCATCAGATACAGGAAGTAACGATGCTCACAATA
  AAAAAGCAGTTTCCAGATCAGCTGAACAGCAGCCATCAGAGAAATCAACAGAACCAAAGACTAAACCACA
  AGACATGATTTCTGCTGGTGGAGAGAGTGTTGCTGGTATCACTGCAATATCTGGCAAGCCGGGTGACAAG
  AAAAAAGAAAAGAAATCATTAACCCCAGCTGTGCCAGTTGAATCTAAACCGGATAAACCATCGGGAAAGT
  CAGGCATGGATGCTGCTTTGGATGACTTAATAGATACTTTAGGAGGACCTGAAGAAACTGAAGAAGAAAA
  TACAACGTATACTGGACCAGAAGTTTCAGATCCAATGAGTTCCACCTACATAGAGGAATTGGGTAAAAGA
  GAAGTCACAATTCCTCCAAAATATAGGGAACTATTGGCTAAAAAGGAAGGGATCACAGGGCCTCCTGCAG
  ACTCTTCGAAACCCATAGGGCCAGATGATGCTATAGACGCCTTGTCATCTGACTTCACCTGTGGGTCGCC
  TACAGCTGCTGGAAAGAAAACTGAAAAAGAGGAATCTACAGAAGTTTTAAAAGCTCAGTCAGCAGGGACA
  GTCAGAAGTGCTGCTCCACCCCAAGAGAAGAAAAGAAAGGTGGAGAAGGATACAATGAGTGATCAAGCAC
  TCGAGGCTCTGTCGGCTTCACTGGGCACCCGGCAAGCAGAACCTGAGCTCGACCTCCGCTCAATTAAGGA
  AGTCGATGAGGCAAAAGCTAAAGAAGAAAAACTAGAGAAGTGTGGTGAGGATGATGAAACAATCCCATCT
  GAGTACAGATTAAAACCAGCCACGGATAAAGATGGAAAACCACTATTGCCAGAGCCTGAAGAAAAACCCA
  AGCCTCGGAGTGAATCAGAACTCATTGATGAACTTTCAGAAGATTTTGACCGGTCTGAATGTAAAGAGAA
  ACCATCTAAGCCAACTGAAAAGACAGAAGAATCTAAGGCCGCTGCTCCAGCTCCTGTGTCGGAGGCTGTG
  TGTCGGACCTCCATGTGTAGTATACAGTCAGCACCCCCTGAGCCGGCTACCTTGAAGGGCACAGTGCCAG
  ATGATGCTGTAGAAGCCTTGGCTGATAGCCTGGGGAAAAAGGAAGCAGATCCAGAAGATGGAAAACCTGT
  GATGGATAAAGTCAAGGAGAAGGCCAAAGAAGAAGACCGTGAAAAGCTTGGTGAAAAAGAAGAAACAATT
  CCTCCTGATTATAGATTAGAAGAGGTCAAGGATAAAGATGGAAAGCCACTCCTGCCAAAAGAGTCTAAGG
  AACAGCTTCCACCCATGAGTGAAGACTTCCTTCTGGATGCTTTGTCTGAGGACTTCTCTGGTCCACAAAA
  TGCTTCATCTCTTAAATTTGAAGATGCTAAACTTGCTGCTGCCATCTCTGAAGTGGTTTCCCAAACCCCA
  GCTTCAACGACCCAAGCTGGAGCCCCACCCCGTGATACCTCGCAGAGTGACAAAGACCTCGATGATGCCT
  TGGATAAACTCTCTGACAGTCTAGGACAAAGGCAGCCTGACCCAGATGAGAACAAACCAATGGAAGATAA
  AGTAAAGGAAAAAGCTAAAGCTGAACATAGAGACAAGCTTGGAGAAAGAGATGACACTATCCCACCTGAA
  TACAGACATCTCCTGGATGATAATGGACAGGACAAACCAGTGAAGCCACCTACAAAGAAATCAGAGGATT
  CAAAGAAACCTGCAGATGACCAAGACCCCATTGATGCTCTCTCAGGAGATCTGGACAGCTGTCCCTCCAC
  TACAGAAACCTCACAGAACACAGCAAAGGATAAGTGCAAGAAGGCTGCTTCCAGCTCCAAAGCACCTAAG
  AATGGAGGTAAAGCGAAGGATTCAGCAAAGACAACAGAGGAAACTTCCAAGCCAAAAGATGACTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001750
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001750.5, NP_001741.4
• RefSeq Size: 4629 bp
• RefSeq ORF: 2376 bp
• Locus ID: 831
• Cytogenetics: 5q15
• Domains: Calpain_inhib
• Gene Summary: 'The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2010]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.