TRPS1 (NM_014112) Human Untagged Clone

CAT#: SC318587

TRPS1 (untagged)-Human trichorhinophalangeal syndrome I (TRPS1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TRPS1
• Synonyms: GC79; LGCR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_014112, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCTTATGAAGTCAATGCTGGGTATGATTTTACAAATATGGTCCGGAAAAAGAACCCCCCTCTGAGAA
  ACGTTGCAAGTGAAGGCGAGGGCCAGATCCTGGAGCCTATAGGTACAGAAAGCAAGGTATCTGGAAAGAA
  CAAAGAATTTTCTGCAGATCAGATGTCAGAAAATACGGATCAGAGTGATGCTGCAGAACTAAATCATAAG
  GAGGAACATAGCTTGCATGTTCAAGATCCATCTTCTAGCAGTAAGAAGGACTTGAAAAGCGCAGTTCTGA
  GTGAGAAGGCTGGCTTCAATTATGAAAGCCCCAGTAAGGGAGGAAACTTTCCCTCCTTTCCGCATGATGA
  GGTGACAGACAGAAATATGTTGGCTTTCTCATCTCCAGCTGCTGGGGGAGTCTGTGAGCCCTTGAAGTCT
  CCGCAAAGAGCAGAGGCAGATGACCCTCAAGATATGGCCTGCACCCCCTCAGGGGACTCACTGGAGACAA
  AGGAAGATCAGAAGATGTCACCAAAGGCTACAGAGGAAACAGGGCAAGCACAGAGTGGTCAAGCCAATTG
  TCAAGGTTTGAGCCCAGTTTCAGTGGCCTCAAAAAACCCACAAGTGCCTTCAGATGGGGGTGTAAGACTG
  AATAAATCCAAAACTGACTTACTGGTGAATGACAACCCAGACCCGGCACCTCTGTCTCCAGAGCTTCAGG
  ACTTTAAATGCAATATCTGTGGATATGGTTACTACGGCAACGACCCCACAGATCTGATTAAGCACTTCCG
  AAAGTATCACTTAGGACTGCATAACCGCACCAGGCAAGATGCTGAGCTGGACAGCAAAATCTTGGCCCTT
  CATAACATGGTGCAGTTCAGCCATTCCAAAGACTTCCAGAAGGTCAACCGTTCTGTGTTTTCTGGTGTGC
  TGCAGGACATCAATTCTTCAAGGCCTGTTTTACTAAATGGGACCTATGATGTGCAGGTGACTTCAGGTGG
  AACATTCATTGGCATTGGACGGAAAACACCAGATTGCCAAGGGAACACCAAGTATTTCCGCTGTAAATTC
  TGCAATTTCACTTATATGGGCAACTCATCCACCGAATTAGAACAACATTTTCTTCAGACTCACCCAAACA
  AAATAAAAGCTTCTCTCCCCTCCTCTGAGGTTGCAAAACCTTCAGAGAAAAACTCTAACAAGTCCATCCC
  TGCACTTCAATCCAGTGATTCTGGAGACTTGGGAAAATGGCAGGACAAGATAACAGTCAAAGCAGGAGAT
  GACACTCCTGTTGGGTACTCAGTGCCCATAAAGCCCCTCGATTCCTCTAGACAAAATGGTACAGAGGCCA
  CCAGTTACTACTGGTGTAAATTTTGTAGTTTCAGCTGTGAGTCATCTAGCTCACTTAAACTGCTAGAACA
  TTATGGCAAGCAGCACGGAGCAGTGCAGTCAGGCGGCCTTAATCCAGAGTTAAATGATAAGCTTTCCAGG
  GGCTCTGTCATTAATCAGAATGATCTAGCCAAAAGTTCAGAAGGAGAGACAATGACCAAGACAGACAAGA
  GCTCGAGTGGGGCTAAAAAGAAGGACTTCTCCAGCAAGGGAGCCGAGGATAATATGGTAACGAGCTATAA
  TTGTCAGTTCTGTGACTTCCGATATTCCAAAAGCCATGGCCCTGATGTAATTGTAGTGGGGCCACTTCTC
  CGTCATTATCAACAGCTCCATAACATTCACAAGTGTACCATTAAACACTGTCCATTCTGTCCCAGAGGAC
  TTTGCAGCCCAGAAAAGCACCTTGGAGAAATTACTTATCCGTTTGCTTGTAGAAAAAGTAATTGTTCCCA
  CTGTGCACTCTTGCTTCTGCACTTGTCTCCTGGGGCGGCTGGAAGCTCGCGAGTCAAACATCAGTGCCAT
  CAGTGTTCATTCACCACCCCTGACGTAGATGTACTCCTCTTTCACTATGAAAGTGTGCATGAGTCCCAAG
  CATCGGATGTCAAACAAGAAGCAAATCACCTGCAAGGATCGGATGGGCAGCAGTCTGTCAAGGAAAGCAA
  AGAACACTCATGTACCAAATGTGATTTTATTACCCAAGTGGAAGAAGAGATTTCCCGACACTACAGGAGA
  GCACACAGCTGCTACAAATGCCGTCAGTGCAGTTTTACAGCTGCCGATACTCAGTCACTACTGGAGCACT
  TCAACACTGTTCACTGCCAGGAACAGGACATCACTACAGCCAACGGCGAAGAGGACGGTCATGCCATATC
  CACCATCAAAGAGGAGCCCAAAATTGACTTCAGGGTCTACAATCTGCTAACTCCAGACTCTAAAATGGGA
  GAGCCAGTTTCTGAGAGTGTGGTGAAGAGAGAGAAGCTGGAAGAGAAGGACGGGCTCAAAGAGAAAGTTT
  GGACCGAGAGTTCCAGTGATGACCTTCGCAATGTGACTTGGAGAGGGGCAGACATCCTGCGGGGGAGTCC
  GTCATACACCCAAGCAAGCCTGGGGCTGCTGACGCCTGTGTCTGGCACCCAAGAGCAGACAAAGACTCTA
  AGGGATAGTCCCAATGTGGAGGCCGCCCATCTGGCGCGACCTATTTATGGCTTGGCTGTGGAAACCAAGG
  GATTCCTGCAGGGGGCGCCAGCTGGCGGAGAGAAGTCTGGGGCCCTCCCCCAGCAGTATCCTGCATCGGG
  AGAAAACAAGTCCAAGGATGAATCCCAGTCCCTGTTACGGAGGCGTAGAGGCTCCGGTGTTTTTTGTGCC
  AATTGCCTGACCACAAAGACCTCTCTCTGGCGAAAGAATGCAAATGGCGGATATGTATGCAACGCGTGTG
  GCCTCTACCAGAAGCTTCACTCGACTCCCAGGCCTTTAAACATCATTAAACAAAACAACGGTGAGCAGAT
  TATTAGGAGGAGAACAAGAAAGCGCCTTAACCCAGAGGCACTTCAGGCTGAGCAGCTCAACAAACAGCAG
  AGGGGCAGCAATGAGGAGCAAGTCAATGGAAGCCCGTTAGAGAGGAGGTCAGAAGATCATCTAACTGAAA
  GTCACCAGAGAGAAATTCCACTCCCCAGCCTAAGTAAATACGAAGCCCAGGGTTCATTGACTAAAAGCCA
  TTCTGCTCAGCAGCCAGTCCTGGTCAGCCAAACTCTGGATATTCACAAAAGGATGCAACCTTTGCACATT
  CAGATAAAAAGTCCTCAGGAAAGTACTGGAGATCCAGGAAATAGTTCATCCGTATCTGAAGGGAAAGGAA
  GTTCTGAGAGAGGCAGTCCTATAGAAAAGTACATGAGACCTGCGAAACACCCAAATTATTCACCACCAGG
  CAGCCCTATTGAAAAGTACCAGTACCCACTTTTTGGACTTCCCTTTGTACATAATGACTTCCAGAGTGAA
  GCTGATTGGCTGCGGTTCTGGAGTAAATATAAGCTCTCCGTTCCTGGGAATCCGCACTACTTGAGTCACG
  TGCCTGGCCTACCAAATCCTTGCCAAAACTATGTGCCTTATCCCACCTTCAATCTGCCTCCTCATTTTTC
  AGCTGTTGGATCAGACAATGACATTCCTCTAGATTTGGCGATCAAGCATTCCAGACCTGGGCCAACTGCA
  AACGGTGCCTCCAAGGAGAAAACGAAGGCACCACCAAATGTAAAAAATGAAGGTCCCTTGAATGTAGTAA
  AAACAGAGAAAGTTGATAGAAGTACTCAAGATGAACTTTCAACAAAATGTGTGCACTGTGGCATTGTCTT
  TCTGGATGAAGTGATGTATGCTTTGCATATGAGTTGCCATGGTGACAGTGGACCTTTCCAGTGCAGCATA
  TGCCAGCATCTTTGCACGGACAAATATGACTTCACAACACATATCCAGAGGGGCCTGCATAGGAACAATG
  CACAAGTGGAAAAAAATGGAAAACCTAAAGAGTAA
  
  
• Restriction Sites: SgfI-RsrII
• ACCN: NM_014112
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014112.4, NP_054831.2
• RefSeq Size: 10017 bp
• RefSeq ORF: 3885 bp
• Locus ID: 7227
• Cytogenetics: 8q23.3
• Domains: GATA, zf-C2H2
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'This gene encodes a transcription factor that represses GATA-regulated genes and binds to a dynein light chain protein. Binding of the encoded protein to the dynein light chain protein affects binding to GATA consensus sequences and suppresses its transcriptional activity. Defects in this gene are a cause of tricho-rhino-phalangeal syndrome (TRPS) types I-III. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.