CCBL1 (KYAT1) (NM_001122671) Human Untagged Clone

CAT#: SC318869

CCBL1 (untagged)-Human cysteine conjugate-beta lyase, cytoplasmic (CCBL1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KYAT1
• Synonyms: CCBL1; GTK; KAT1; KATI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001122671, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCAAACAGCTGCAGGCCCGAAGGCTAGACGGGATCGACTACAACCCCTGGGTGGAGTTTGTGAAAC
  TGGCCAGTGAGCATGACGTCGTGAACTTGGGCCAGGGCTTCCCGGATTTCCCACCACCAGACTTTGCCGT
  GGAAGCCTTTCAGCACGCTGTCAGTGGAGACTTCATGCTTAACCAGTACACCAAGACATTTGGTTACCCA
  CCACTGACGAAGATCCTGGCAAGTTTCTTTGGGGAGCTGCTGGGTCAGGAGATAGACCCGCTCAGGAATG
  TGCTGGTGACTGTTGGTGGCTATGGGGCCCTGTTCACAGCCTTCCAGGCCCTGGTGGACGAAGGAGACGA
  GGTCATCATCATCGAACCCTTTTTTGACTGCTACGAGCCCATGACAATGATGGCAGGGGGTCGTCCTGTG
  TTTGTGTCCCTGAAGCCGGGTCCCATCCAGAATGGAGAACTGGGTTCCAGCAGCAACTGGCAGCTGGACC
  CCATGGAGCTGGCCGGCAAATTCACATCACGCACCAAAGCCCTGGTCCTCAACACCCCCAACAACCCCCT
  GGGCAAGGTGTTCTCCAGGGAAGAGCTGGAGCTGGTGGCCAGCCTTTGCCAGCAGCATGACGTGGTGTGT
  ATCACTGATGAAGTCTACCAGTGGATGGTCTACGACGGGCACCAGCACATCAGCATTGCCAGCCTCCCTG
  GCATGTGGGAACGGACCCTGACCATCGGCAGCGCCGGCAAGACCTTCAGCGCCACTGGCTGGAAGGTGGG
  CTGGGTCCTGGGTCCAGATCACATCATGAAGCACCTGCGGACCGTGCACCAGAACTCCGTCTTCCACTGC
  CCCACGCAGAGCCAGGCTGCAGTAGCCGAGAGCTTTGAACGGGAGCAGCTGCTCTTCCGCCAACCCAGCA
  GCTACTTTGTGCAGTTCCCGCAGGCCATGCAGCGCTGCCGTGACCACATGATACGTAGCCTACAGTCAGT
  GGGCCTGAAGCCCATCATCCCTCAGGGCAGCTACTTCCTCATCACAGACATCTCAGACTTCAAGAGGAAG
  ATGCCTGACTTGCCTGGAGCTGTGGATGAGCCCTATGACAGACGCTTCGTCAAGTGGATGATCAAGAACA
  AGGGCTTGGTGGCCATCCCTGTCTCCATCTTCTATAGTGTGCCACATCAGAAGCACTTTGACCACTATAT
  CCGCTTCTGTTTTGTGAAGGATGAAGCCACGCTCCAGGCCATGGACGAGAAGCTGCGGAAGTGGAAGGTG
  GAACTCTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001122671
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001122671.1, NP_001116143.1
• RefSeq Size: 1936 bp
• RefSeq ORF: 1269 bp
• Locus ID: 883
• Cytogenetics: 9q34.11
• Gene Summary: 'This gene encodes a cytosolic enzyme that is responsible for the metabolism of cysteine conjugates of certain halogenated alkenes and alkanes. This metabolism can form reactive metabolites leading to nephrotoxicity and neurotoxicity. Increased levels of this enzyme have been linked to schizophrenia. Multiple transcript variants that encode different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, and 6-11 all encode the same isoform (a).