CD105 (ENG) (NM_001114753) Human Untagged Clone

CAT#: SC318963

ENG (untagged)-Human endoglin (ENG), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ENG
• Synonyms: END; HHT1; ORW1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001114753, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGACCGCGGCACGCTCCCTCTGGCTGTTGCCCTGCTGCTGGCCAGCTGCAGCCTCAGCCCCACAAGTC
  TTGCAGAAACAGTCCATTGTGACCTTCAGCCTGTGGGCCCCGAGAGGGGCGAGGTGACATATACCACTAG
  CCAGGTCTCGAAGGGCTGCGTGGCTCAGGCCCCCAATGCCATCCTTGAAGTCCATGTCCTCTTCCTGGAG
  TTCCCAACGGGCCCGTCACAGCTGGAGCTGACTCTCCAGGCATCCAAGCAAAATGGCACCTGGCCCCGAG
  AGGTGCTTCTGGTCCTCAGTGTAAACAGCAGTGTCTTCCTGCATCTCCAGGCCCTGGGAATCCCACTGCA
  CTTGGCCTACAATTCCAGCCTGGTCACCTTCCAAGAGCCCCCGGGGGTCAACACCACAGAGCTGCCATCC
  TTCCCCAAGACCCAGATCCTTGAGTGGGCAGCTGAGAGGGGCCCCATCACCTCTGCTGCTGAGCTGAATG
  ACCCCCAGAGCATCCTCCTCCGACTGGGCCAAGCCCAGGGGTCACTGTCCTTCTGCATGCTGGAAGCCAG
  CCAGGACATGGGCCGCACGCTCGAGTGGCGGCCGCGTACTCCAGCCTTGGTCCGGGGCTGCCACTTGGAA
  GGCGTGGCCGGCCACAAGGAGGCGCACATCCTGAGGGTCCTGCCGGGCCACTCGGCCGGGCCCCGGACGG
  TGACGGTGAAGGTGGAACTGAGCTGCGCACCCGGGGATCTCGATGCCGTCCTCATCCTGCAGGGTCCCCC
  CTACGTGTCCTGGCTCATCGACGCCAACCACAACATGCAGATCTGGACCACTGGAGAATACTCCTTCAAG
  ATCTTTCCAGAGAAAAACATTCGTGGCTTCAAGCTCCCAGACACACCTCAAGGCCTCCTGGGGGAGGCCC
  GGATGCTCAATGCCAGCATTGTGGCATCCTTCGTGGAGCTACCGCTGGCCAGCATTGTCTCACTTCATGC
  CTCCAGCTGCGGTGGTAGGCTGCAGACCTCACCCGCACCGATCCAGACCACTCCTCCCAAGGACACTTGT
  AGCCCGGAGCTGCTCATGTCCTTGATCCAGACAAAGTGTGCCGACGACGCCATGACCCTGGTACTAAAGA
  AAGAGCTTGTTGCGCATTTGAAGTGCACCATCACGGGCCTGACCTTCTGGGACCCCAGCTGTGAGGCAGA
  GGACAGGGGTGACAAGTTTGTCTTGCGCAGTGCTTACTCCAGCTGTGGCATGCAGGTGTCAGCAAGTATG
  ATCAGCAATGAGGCGGTGGTCAATATCCTGTCGAGCTCATCACCACAGCGGAAAAAGGTGCACTGCCTCA
  ACATGGACAGCCTCTCTTTCCAGCTGGGCCTCTACCTCAGCCCACACTTCCTCCAGGCCTCCAACACCAT
  CGAGCCGGGGCAGCAGAGCTTTGTGCAGGTCAGAGTGTCCCCATCCGTCTCCGAGTTCCTGCTCCAGTTA
  GACAGCTGCCACCTGGACTTGGGGCCTGAGGGAGGCACCGTGGAACTCATCCAGGGCCGGGCGGCCAAGG
  GCAACTGTGTGAGCCTGCTGTCCCCAAGCCCCGAGGGTGACCCGCGCTTCAGCTTCCTCCTCCACTTCTA
  CACAGTACCCATACCCAAAACCGGCACCCTCAGCTGCACGGTAGCCCTGCGTCCCAAGACCGGGTCTCAA
  GACCAGGAAGTCCATAGGACTGTCTTCATGCGCTTGAACATCATCAGCCCTGACCTGTCTGGTTGCACAA
  GCAAAGGCCTCGTCCTGCCCGCCGTGCTGGGCATCACCTTTGGTGCCTTCCTCATCGGGGCCCTGCTCAC
  TGCTGCACTCTGGTACATCTACTCGCACACGCGTTCCCCCAGCAAGCGGGAGCCCGTGGTGGCGGTGGCT
  GCCCCGGCCTCCTCGGAGAGCAGCAGCACCAACCACAGCATCGGGAGCACCCAGAGCACCCCCTGCTCCA
  CCAGCAGCATGGCATAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001114753 unedited
  TTGGATTTTGTAATACGACTCACTATAGGGCGGGCCGCGAATCGGCACGAGGGCCGGCCG
  GGCTGGATGAGCCGGGAGCTCCCTGCTGCCGGTCATACCACAGCCTTCATCTGCGCCCTG
  GGGCCAGGACTGCTGCTGTCACTGCCATCCATTGGAGCCCAGCACCCCCTCCCCGCCCAT
  CCTTCGGACAGCAACTCCAGCCCAGCCCCGCGTCCCTGTGTCCACTTCTCCTGACCCCTC
  GGCCGCCACCCCAGAAGGCTGGAGCAGGGACGCCGTCGCTCCGGCCGCCTGCTCCCCTCG
  GGTCCCCGTGCGAGCCCACGCCGGCCCCGGTGCCCGCCCGCAGCCCTGCCACTGGACACA
  GGATAAGGCCCAGCGCACAGGCCCCCACGTGGACAGCATGGACCGCGGCACGCTCCCTCT
  GGCTGTTGCCCTGCTGCTGGCCAGCTGCAGCCTCAGCCCCACAAGTCTTGCAGAAACAGT
  CCATTGTGACCTTCAGCCTGTGGGCCCCGAGAGGGGCGAGGTGACATATACCACTAGCCA
  GGTCTCGAAGGGCTGCGTGGCTCAGGCCCCCAATGCCATCCTTTGAAGTCCATGTCCTCT
  TCCTAGAGTTCCCAACGGGCCCGTCACAGCTNGGAGCTGACTCTCCAGGCATCCAAGCAA
  AATGGCACCTGCCCCCGAGAGGGGCCTTCTGGNCCTCAGTGTAAACAACAAGTGTCTTCC
  TGCATCTCCAGGCCCTGGGAATCCCACTGCACTTGGCCTACAATTTCAGCCTGGTCACCT
  TCCAGACCCCCCGGGGGGTAACACCACAGAGCTGGCATCCTTCCCAAGACCAATCCTTTG
  AGTGGGCAGTTGAGGAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001114753 unedited
  GCCGCCTCTAAAGTGAGTTTTTTTTTTTTTTTTTTGGTTTCATGGTCTGATTTATTGGTG
  GTGAATACACAGGGGCAGGCCCAGGACAAGCAGCTTGGCTACTCCCCCTCTGCTGGCTGC
  CCGACCGGCAGAGGGGGCTCCATGTGGCAGGAGCTAGGCTCCCAACGCCCACTGTTCTTG
  CCACCCTCTGGGCTCCCATGCTGGGCTCCGCTAGGCTCCTGTCTCCCCTGCCAGTTAGTT
  AGGCAAGTTCAGGTGTGGAGGCCGCAGGGATAGATCCAGGTGGCTCTGGGCTGGGCCCTC
  TTCTCTTCCCAGCGGGGAGGTGCTGTTGGCCTGGCTGGGCTGGCCTGAATCTGTTTCAAG
  TTCTCCCTTCCTGCCCAGCTCAGTTCACCAGTGCTGGATCCAGGTTCAAATGACAGGGAC
  TTGGGTTTTTACAACAGCGTGGCAAGTGGTCTGTCTCCTGGGCAGCCATATCCCAGACCC
  ACTGGGTTGAAAGTTCTGTGGGGTGGAGGGACCCCAAGGTGTTCCAAGCCAGTGGCTGCA
  CTGGCAGCAGGCCTCTGATAGGGAGGCGGGAAGGGTAAGCGCGGAGAGCAAGCTCCATTC
  TGGGTCGAGTGGAGGACTGGCTCCCAGGGTGAGTTCACACCAGTGCTCCCAGCTGGCGGC
  TGCTCAGTCTCTCCTGCTGGGCGAGCGCGGGGGGCCGGAGCCTATGCCATGCTGCTGGTG
  GAGCAAGAGGTGCCCTGGGAGCTCCCGATGCTGGAGGTGGCGCTGCTGCTCTCCGAGAAG
  GCCGGTGCAGGCACCCGCCCCACGGCTTCCGCTTGCTGAGGGAACCGTGCGCGCATATAG
  TCCCCATGCCACATGATCAGGCCCCGATGAGGATGCCCCTAGTGATGCCCACACGCGGGC
  AGTCAGGCCTTTCTGGGAACAAAAGATAGGTTGTGAGTTTACCCATAAACCCTCCGGCCC
  TCGGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001114753
• Insert Size: 3000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001114753.1, NP_001108225.1
• RefSeq Size: 3077 bp
• RefSeq ORF: 1977 bp
• Locus ID: 2022
• Cytogenetics: 9q34.11
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Gene Summary: 'This gene encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds to the beta1 and beta3 peptides with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia. This gene may also be involved in preeclampsia and several types of cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013]'
  Transcript Variant: This variant (1) encodes the longer isoform (1, also known as L-endoglin).