Centrin 3 (CETN3) (NM_004365) Human Untagged Clone

CAT#: SC319201

CETN3 (untagged)-Human centrin, EF-hand protein, 3 (CETN3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CETN3
• Synonyms: CDC31; CEN3
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004365.2 GTCGTTCGTGTCTGTTGAACGGCTGTGGGCGTCTTGCTGCCTTGGGTAGGGGGTTAAAAT
  CGTTCTTGAGAGGAACGTCTCTGTGCGAAGAGATAATGAGTTTAGCTCTGAGAAGTGAGC
  TTGTAGTGGACAAAACAAAGAGGAAAAAAAGAAGAGAACTGTCTGAGGAACAGAAACAAG
  AAATTAAAGATGCTTTTGAACTATTTGATACAGACAAAGATGAAGCAATAGATTATCATG
  AATTAAAGGTGGCAATGAGAGCCTTGGGGTTTGATGTAAAAAAAGCTGATGTACTGAAGA
  TTCTTAAAGATTATGACAGAGAAGCCACAGGGAAAATCACCTTTGAAGATTTTAATGAAG
  TTGTGACAGACTGGATATTGGAAAGAGATCCCCATGAAGAAATACTCAAGGCATTTAAAC
  TATTTGATGATGATGATTCAGGTAAAATAAGCTTGAGGAATTTGCGACGTGTTGCTAGAG
  AATTGGGTGAAAACATGAGTGATGAAGAACTTCGAGCTATGATAGAAGAATTTGACAAAG
  ATGGTGATGGAGAAATAAACCAAGAGGAGTTCATTGCTATTATGACTGGTGACATTTAAA
  GAATTACAAGGATAAACACTAAGAATGTTGCAGTTACCATCTTATATTCTATTTTTGTGC
  CTGGAGCCATGTGAAAAAAACCAACTTAGTTCTTTTATCCTAAAGGACCAAAAATAAGCA
  TCTTATATATCTGTATTTTACTACTGTTAAGTTTCTTTGTATGAACTGTGTTGTTAGTAC
  TCAGATAGTTTAGCTTTGTATTTATAATAGAGCTTTTATATAAAGTTTTAAAAATTGAAT
  GTGTGATATGTGTTCTTTGAAGGTTTTTTAATTTAACATTTATAGTCACTTTTTAGTGCA
  CACATTTTCCAGACTTTCGTTAATAAAATATTTATTTTATTAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_004365
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004365.2, NP_004356.2
• RefSeq Size: 997 bp
• RefSeq ORF: 504 bp
• Locus ID: 1070
• Cytogenetics: 5q14.3
• Domains: EFh
• Protein Families: Druggable Genome
• Gene Summary: 'The protein encoded by this gene contains four EF-hand calcium binding domains, and is a member of the centrin protein family. Centrins are evolutionarily conserved proteins similar to the CDC31 protein of S. cerevisiae. Yeast CDC31 is located at the centrosome of interphase and mitotic cells, where it plays a fundamental role in centrosome duplication and separation. Multiple forms of the proteins similar to the yeast centrin have been identified in human and other mammalian cells, some of which have been shown to be associated with centrosome fractions. This protein appears to be one of the most abundant centrins associated with centrosome, which suggests a similar function to its yeast counterpart. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]'
  Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1.