TRIM5 alpha (TRIM5) (NM_033092) Human Untagged Clone

CAT#: SC321632

TRIM5 (untagged)-Human tripartite motif containing 5 (TRIM5), transcript variant gamma

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TRIM5
• Synonyms: RNF88; TRIM5alpha
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_033092.1 CTTTGTGCAGATCAGGCCCGTGGATTGGTGAGTGAATCCTAACCACGTCTTCCCTGGCCT
  GTCTTCACTCTTCTCCCCAGAATCACCACTTCTGCACTGGTGTCTGAAGGTGTATTGAGT
  GATTTTGTGGAGGGCAGAAGTAGGAAGTCTTTGGGACAAAACTGTATTTACCTTGGGATC
  TGTGAACAAGAGGAACCTCAGCAGCCAGGACAGGCAGGAGCAGTGGAATAGCTAGTATGG
  CTTCTGGAATCCTGGTTAATGTAAAGGAGGAGGTGACCTGCCCCATCTGCCTGGAACTCC
  TGACACAACCCCTGAGCCTGGACTGCGGCCACAGCTTCTGCCAAGCATGCCTCACTGCAA
  ACCACAAGAAGTCCATGCTAGACAAAGGAGAGAGTAGCTGCCCTGTGTGCCGGATCAGTT
  ACCAGCCTGAGAACATACGGCCTAATCGGCATGTAGCCAACATAGTGGAGAAGCTCAGGG
  AGGTCAAGTTGAGCCCAGAGGGGCAGAAAGTTGATCATTGTGCACGCCATGGAGAGAAAC
  TTCTACTCTTCTGTCAGGAGGACGGGAAGGTCATTTGCTGGCTTTGTGAGCGGTCTCAGG
  AGCACCGTGGTCACCACACGTTCCTCACAGAGGAGGTTGCCCGGGAGTACCAAGTGAAGC
  TCCAGGCAGCTCTGGAGATGCTGAGGCAGAAGCAGCAGGAAGCTGAAGAGTTGGAAGCTG
  ACATCAGAGAAGAGAAAGCTTCCTGGAAGACTCAAATACAGTATGACAAAACCAACGTCT
  TGGCAGATTTTGAGCAACTGAGAGACATCCTGGACTGGGAGGAGAGCAATGAGCTGCAAA
  ACCTGGAGAAGGAGGAGGAAGACATTCTGAAAAGCCTTACGAACTCTGAAACTGAGATGG
  TGCAGCAGACCCAGTCCCTGAGAGAGCTCATCTCAGATCTGGAGCATCGGCTGCAGGGGT
  CAGTGATGGAGCTGCTTCAGGGTGTGGATGGCGTCATAAAAAGGACGGAGAACGTGACCT
  TGAAGAAGCCAGAAACTTTTCCAAAAAATCAAAGGAGAGTGTTTCGAGCTCCTGATCTGA
  AAGGAATGCTAGAAGTGTTTAGAGAGCTGACAGATGTCCGACGCTACTGGGGTAAGGAGA
  AGTCACATTATCATAAGCCACCCTGCGGCTTATCATTATTATTATCTTTATCTTTTAGAA
  TTTTATGTTCTCTATTAGGCTCATGTTTTAAGATTTATGATTCTCCTTCCAAGACACACA
  TAACTTACCCCTCCTTATAACTTCTAAACAAGGTTCCTCCCAGTTTTCTCTCAAGTCTTT
  ATCAAGATTTCTCTCATATCACAAATAAAGTTACATTATATCCCTTAAAAAAAAAAAAAA
  AAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_033092.1 unedited
  GGCGATTGTATACGACTCCTATAGGGCGGCCGGGAATTCGGCACGCAGGCTTTGTGCAGA
  TCAGGCCCGTGGATTGGTGAGTGAATCCTAACCACGTCTTCCCTGGCCTGTCTTCACTCT
  TCTCCCCAGAATCACCACTTCTGCACTGGTGTCTGAAGGTGTATTGAGTGATTTTGTGGA
  GGGCAGAAGTAGGAAGTCTTTGGGACAAAACTGTATTTACCTTGGGATCTGTGAACAAGA
  GGAACCTCAGCAGCCAGGACAGGCAGGAGCAGTGGAATAGCTAGTATGGCTTCTGGAATC
  CTGGTTAATGTAAAGGAGGAGGTGATCTGTCCCATCTGCCTGGAACTCCTGACACAACCC
  CTGAGCCTGGACTGCGGCCACAGCTTCTGCCAAGCATGCCTCACTGCAAACCACAAGAAG
  TCCATGCTAGACAAAGGAGAGAGTAGCTGCCCTGTGTGCCGGATCAGTTACCAGCCTGAG
  AACATACGGCCTAATCGGCATGTAGCCAACATAGTGGAGAAGCTCAGGGAGGTCAAGTTG
  AGCCCAGAGGGGCAGAAAGTTGATCATTGTGCACGCCATGGAGAGAAACTTCTACTCTTC
  TGTCAGAGGACGGGAAAGGTCATTTGCTGGCTTTGTGAGCGGTCTCAGGAGCACCGTGGT
  CACCACACGTTCCTCACAGAGGAGGTTGCCCGGGAGTACCAAGTGAAGCTCCAGGCAGCT
  CTGGAGATGCTGAGGCAGAGCAGCAGAAGCTGAGAGTTGGAAGCTGACATCAGAGAAGAG
  AAAGCTTCCTGGAGACTCAAAATACAGTTATGACAAAACCACGTTCTTGGCAGATTTTGA
  GCAACTGAAGAGACTCCTGGACTGGGAGGGAGAGCATGGAGCTGCAAAACCCTGGAGAAA
  GGAGGGAGGGAGAGACACT
• Restriction Sites: Please inquire
• ACCN: NM_033092
• ORF Size: 1044 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_033092.1, NP_149083.1
• RefSeq Size: 1686
• RefSeq ORF: 1044
• Locus ID: 85363
• Domains: zf-B_box, RING
• Protein Families: Druggable Genome
• Gene Summary: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein forms homo-oligomers via the coilel-coil region and localizes to cytoplasmic bodies. It appears to function as a E3 ubiquitin-ligase and ubiqutinates itself to regulate its subcellular localization. It may play a role in retroviral restriction. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2009]
  Transcript Variant: This variant (gamma) includes an alternate segment in the 3' coding region, compared to variant alpha, which results in a frameshift. The resulting protein (isoform gamma) has a shorter and distinct C-terminus, compared to isoform alpha. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.