Rad9 (RAD9A) (NM_004584) Human Untagged Clone

CAT#: SC321679

RAD9A (untagged)-Human RAD9 homolog A (S. pombe) (RAD9A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RAD9A
• Synonyms: RAD9
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004584.2 GGGCCGGCAGGGGCGGTGCGCGGGAAGGGACCCCGGACCCGGAGGTCGCGGAGAGCTGGG
  CAGTGTTGGCCGCTGGCGGAGCGCTGGGGCAGCATGAAGTGCCTGGTCACGGGCGGCAAC
  GTGAAGGTGCTCGGCAAGGCCGTCCACTCCCTGTCCCGCATCGGGGACGAGCTCTACCTG
  GAACCCTTGGAGGACGGGCTCTCCCTCCGGACGGTGAACTCCTCCCGCTCTGCCTATGCC
  TGCTTTCTCTTTGCCCCGCTCTTCTTCCAGCAATACCAGGCAGCCACCCCTGGTCAGGAC
  CTGCTGCGCTGTAAGATCCTGATGAAGTCTTTCCTGTCTGTCTTCCGCTCACTGGCGATG
  CTGGAGAAGACGGTGGAAAAATGCTGCATCTCCCTGAATGGCCGGAGCAGCCGCCTGGTG
  GTCCAGCTGCATTGCAAGTTCGGGGTGCGGAAGACTCACAACCTGTCCTTCCAGGACTGT
  GCGTCCCTGCAGGCCGTCTTCGACCCAGCCTCGTGCCCCCACATGCTCCGCGCCCCAGCA
  CGGGTTCTGGGGGAGGCTGTTCTGCCCTTCTCTCCTGCACTGGCTGAAGTGACGCTGGGC
  ATTGGCCGTGGCCGCAGGGTCATCCTGCGCAGCTACCACGAGGAGGAGGCAGACAGCACT
  GCCAAAGCCATGGTGACTGAGATGTGCCTTGGAGAGGAGGATTTCCAGCAGCTGCAGGCC
  CAGGAAGGGGTGGCCATCACTTTCTGCCTCAAGGAATTCCGGGGGCTCCTGAGCTTTGCA
  GAGTCAGCAAACTTGAATCTTAGCATTCATTTTGATGCTCCAGGCAGGCCCGCCATCTTC
  ACCATCAAGGACTCTTTGCTGGACGGCCACTTTGTCTTGGCCACACTCTCAGACACCGAC
  TCGCACTCCCAGGACCTGGGCTCCCCAGAGCGTCACCAGCCAGTGCCTCAGCTCCAGGCT
  CACAGCACACCCCACCCGGACGACTTTGCCAATGACGACATTGACTCTTACATGATCGCC
  ATGGAAACCACTATAGGCAATGAGGGCTCGCGGGTGCTGCCCTCCATTTCCCTTTCACCT
  GGCCCCCAGCCCCCCAAGAGCCCCGGTCCCCACTCCGAGGAGGAAGATGAGGCTGAGCCC
  AGTACAGTGCCTGGGACTCCCCCACCCAAGAAGTTCCGCTCACTGTTCTTCGGCTCCATC
  CTGGCCCCTGTACGCTCCCCCCAGGGCCCCAGCCCTGTGCTGGCGGAAGACAGTGAGGGT
  GAAGGCTGAACCAAGAACCTGAAGCCTGTACCCAGAGGCCTTGGACTAGACGAAGCCCCA
  GCCAGTGGCAGAACTGGGTCTCTCAGCCCTGGGGATCAGAAAGGTGGGCTTGCTGGAGCT
  GAGCTGTTTCACTGCCTCTCGCAGGCCCCAGCTGGCTGTCACTGTAAAGCTGTCCCACAG
  CGGTCGGGCCTGGGCCGTTATCTCCCCACAACCCCCAGCCAATCAGGACTTTCCAGACTT
  GGCCCTGAACTACTGACGTTCCTACCTCTTATTTCTCATTGAGCCTCAGGCTATACTCCA
  GCTGGCCAAGGCTGGAAACCTGTCTCCCTCAGGCTCACCTTCCTAAGGAAAATGTCATAG
  TAGGTGCTGCTGGCCCCTGGTGAGCCAGCTTCTCTGCCAATCATGACCTGTTCCTTCCTG
  AAGTCCTGGGCATGCATCTGGGACCCCCGTGGAGCTGACAAGTTTTCCTTGCTTTCCTGA
  TACTCTTTGGCGCTGACTTGGAATTCTAAGAGCCTTGGACCCGAGTGTGTGGCTAGGGTT
  GCCCTGGCTGGGGCCCGGTGCCGAGACTCCCAAGCGGCTCTGTGCAGAAGAGCTGCCAGG
  CAGTGTCTTAGATGTGAGACGGAGGCCATGGCGAGAATCCAGCTTTGACCTTTATTCAAG
  AGACCAGATGGGTTGCCCCAGGATCCGGCTGCCAGCCCTGAGGCCAAGCACGGCTGGAGA
  CCCACGACCTGGCCTGCCGTTGCCCTGAGCTGCAGCCTCGGCCCCAGGATCCTGCTCACA
  GTCACCGCAGGTGCAGGCAGGAAGCAGCCCTGGGGGACTGGACGCTGCTATTGATTCATT
  AAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_004584
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004584.2, NP_004575.1
• RefSeq Size: 2128 bp
• RefSeq ORF: 1176 bp
• Locus ID: 5883
• Cytogenetics: 11q13.2
• Domains: Rad9
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Gene Summary: 'This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair. This protein possesses 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]'
  Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longer isoform (1).