MTHFS (NM_006441) Human Untagged Clone

CAT#: SC322547

MTHFS (untagged)-Human 5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase) (MTHFS), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MTHFS
• Synonyms: HsT19268; NEDMEHM
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322547 AGACCGAACCCGAGGGCGCCCAGGGCGCCGAGGGCGGGACTGGACTCGGCTTGGGCGTGA
  GATGGCGGCGGCAGCGGTGAGCAGCGCCAAGCGTAGCCTGCGGGGAGAGCTGAAGCAGCG
  TCTGCGGGCGATGAGTGCCGAGGAGCGGCTACGCCAGTCCCGCGTACTGAGCCAGAAGGT
  GATTGCCCACAGTGAGTATCAAAAGTCCAAAAGAATTTCCATCTTTCTGAGCATGCAAGA
  TGAAATTGAGACAGAAGAGATCATCAAGGACATTTTCCAACGAGGCAAAATCTGCTTCAT
  CCCTCGGTACCGGTTCCAGAGCAATCACATGGATATGGTGAGAATAGAATCACCAGAGGA
  AATTTCTTTACTTCCCAAAACATCCTGGAATATCCCTCAGCCTGGTGAGGGTGATGTTCG
  GGAGGAGGCCTTGTCCACAGGGGGACTTGATCTCATCTTCATGCCAGGCCTTGGGTTTGA
  CAAACATGGCAACCGACTGGGGAGGGGCAAGGGCTACTATGATGCCTATCTGAAGCGCTG
  TTTGCAGCATCAGGAAGTGAAGCCCTACACCCTGGCGTTGGCTTTCAAAGAACAGATTTG
  CCTCCAGGTCCCAGTGAATGAAAACGACATGAAGGTAGATGAAGTCCTTTACGAAGACTC
  GTCAACAGCTTAAATCTGGATTACTACAGCCAAATAATCAGTGTTTTATATGAGAGTAAA
  GCAAAGTATGTGTATTTTTCCCTTGTCAAAAATTAGTTGAAATTGTTCATTAATGTGAAT
  ACAGACTGCATTTTAAAATTGTAATTATGAAATACCTTATATAAAACCATCTTTAAAAAC
  CAAAAAAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for SC322547 unedited
  GTGCTATTTGTATACGACTCCTATAGGGCGGCCGGCGATCGCGGCGCGCCGGTACCGGTC
  CGGAATTCCCGGGATATCGTCGACCCACGCGTCCGAGACCGAACCCGAGGGCGCCCAGGG
  CGCCGAGGGCGGGACTGGACTCGGCTTGGGCGTGAGATGGCGGCGGCAGCGGTGAGCAGC
  GCCAAGCGTAGCCTGCGGGGAGAGCTGAAGCAGCGTCTGCGGGCGATGAGTGCCGAGGAG
  CGGCTACGCCAGTCCCGCGTACTGAGCCAGAAGGTGATTGCCCACAGTGAGTATCAAAAG
  TCCAAAAGAATTTCCATCTTTCTGAGCATGCAAGATGAAATTGAGACAGAAGAGATCATC
  AAGGACATTTTCCAACGAGGCAAAATCTGCTTCATCCCTCGGTACCGGTTCCAGAGCAAT
  CACATGGATATGGTGAGAATAGAATCACCAGAGGAAATTTCTTTACTTCCCAAAACATCC
  TGGAATATCCCTCAGCCTGGTGAGGGTGATGTTCGGGAGGAGGCCTTGTCCACAGGGGGA
  CTTGATCTCATCTTCATGCCAGGCCTTGGGTTTGACAAACATGGCAACCGACTGGGGAGG
  GGCAAGGGCTACTATGATGCCTATCTGAAGCGCTGTTTGCAGCATCAGGAAGTGAAGCCC
  TACACCCTGGCGTTGGCTTTCAAAGAACAGATTTGCCTCCAGGTCCCAGTGAATGAAAAC
  GACATGAAAGTAGATGAAGTCCTTTACGAAGACTCGTCAACAGCTTAAATCTGGATTACT
  ACAGCCCAAATAATCAGTGTTTTTTTATGAGAGTAAAGCAAAGTATGTGTATTTTTCCCT
  TGTCAAAATTAGTGAAATTGTCATTAATGTGAATACAGACTGCATTTTAAAATTGTATTA
  TGAAATACCTTATATAAAAACATCTTTAAAACCAAAAAAAAAAAAAAAAAAAGGGCGGGC
  CGCACCTCGGAGGTTAACCGGCCCGGCCCGCGGGTCCTA
• Restriction Sites: Please inquire
• ACCN: NM_006441
• ORF Size: 612 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_006441.1, NP_006432.1
• RefSeq Size: 857
• RefSeq ORF: 612
• Locus ID: 10588
• Domains: 5-FTHF_cyc-lig
• Protein Pathways: Metabolic pathways, One carbon pool by folate
• Gene Summary: The protein encoded by this gene is an enzyme that catalyzes the conversion of 5-formyltetrahydrofolate to 5,10-methenyltetrahydrofolate, a precursor of reduced folates involved in 1-carbon metabolism. An increased activity of the encoded protein can result in an increased folate turnover rate and folate depletion. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2011]
  Transcript Variant: This variant (1) encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.