PPP6C (NM_001123369) Human Untagged Clone

CAT#: SC322948

PPP6C (untagged)-Human protein phosphatase 6, catalytic subunit (PPP6C), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPP6C
• Synonyms: PP6; PP6C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001123369, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGCCGCTAGACCTGGACAAGTATGTGGAAATAGCGCGGCTGTGCAAGTACCTGCCAGAGAACGACC
  TGAAGCGGCTATGTGACTACGTTTGTGACCTCCTCTTAGAAGAGTCAAATGTTCAGCCAGTATCAACACC
  AGTAACAGTGTGTGGAGATATCCATGGACAGGGTGATTTTGTAGACAGAGGTTACTATAGTTTGGAGACC
  TTCACTTACCTTCTTGCATTAAAGGCTAAATGGCCTGATCGTATTACACTTTTGCGAGGAAATCATGAGA
  GTAGACAGATAACACAGGTCTATGGATTTTATGATGAGTGCCAAACCAAATATGGAAATGCTAATGCCTG
  GAGATACTGTACCAAAGTTTTTGACATGCTCACAGTAGCAGCTTTAATAGATGAGCAGATTTTGTGTGTC
  CATGGTGGTTTATCTCCTGATATCAAAACACTGGATCAAATTCGAACCATCGAACGGAATCAGGAAATTC
  CTCATAAAGGAGCATTTTGTGATCTGGTTTGGTCAGATCCTGAAGATGTGGATACCTGGGCTATCAGTCC
  CCGAGGAGCAGGTTGGCTTTTTGGAGCAAAGGTCACAAATGAGTTTGTTCATATCAACAACTTAAAACTC
  ATCTGCAGAGCACATCAACTAGTGCACGAAGGCTATAAATTTATGTTTGATGAGAAGCTGGTGACAGTAT
  GGTCTGCTCCTAATTACTGCTATCGTTGTGGAAATATTGCTTCGATCATGGTCTTCAAAGATGTAAATAC
  AAGAGAACCAAAGTTATTCCGGGCAGTTCCAGATTCAGAACGTGTTATTCCTCCCAGAACGACAACGCCA
  TATTTCCTTTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001123369
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001123369.1, NP_001116841.1
• RefSeq Size: 4190 bp
• RefSeq ORF: 852 bp
• Locus ID: 5537
• Cytogenetics: 9q33.3
• Protein Families: Druggable Genome, Phosphatase
• Gene Summary: 'This gene encodes the catalytic subunit of protein phosphatase, a component of a signaling pathway regulating cell cycle progression. Splice variants encoding different protein isoforms exist. The pseudogene of this gene is located on chromosome X. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (3) lacks one coding exon compared to variant 1 and encodes isoform c, which is shorter than isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence and transcripts to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.