CYP21A2 (NM_001128590) Human Untagged Clone

CAT#: SC323083

CYP21A2 (untagged)-Human cytochrome P450, family 21, subfamily A, polypeptide 2 (CYP21A2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CYP21A2
• Synonyms: CA21H; CAH1; CPS1; CYP21; CYP21B; P450c21B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001128590, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCTGCTCCTGGGCCTGCTGCTGCTGCTGCCCCTGCTGGCTGGCGCCCGCCTGCTGTGGAACTGGTGGA
  AGCTCCGGAGCCTCCACCTCCCGCCTCTTGCCCCGGGCTTCTTGCACCTGCTGCAGCCCGACCTCCCCAT
  CTATCTGCTTGGCCTGACTCAGAAATTCGGGCCCATCTACAGGCTCCACCTTGGGCTGCAAGACAAGCTG
  GTGTCTAGGAACTACCCGGACCTGTCCTTGGGAGACTACTCCCTGCTCTGGAAAGCCCACAAGAAGCTCA
  CCCGCTCAGCCCTGCTGCTGGGCATCCGTGACTCCATGGAGCCAGTGGTGGAGCAGCTGACCCAGGAGTT
  CTGTGAGCGCATGAGAGCCCAGCCCGGCACCCCTGTGGCCATTGAGGAGGAATTCTCTCTCCTCACCTGC
  AGCATCATCTGTTACCTCACCTTCGGAGACAAGATCAAGGACGACAACTTAATGCCTGCCTATTACAAAT
  GTATCCAGGAGGTGTTAAAAACCTGGAGCCACTGGTCCATCCAAATTGTGGACGTGATTCCCTTTCTCAG
  GTTCTTCCCCAATCCAGGTCTCCGGAGGCTGAAGCAGGCCATAGAGAAGAGGGATCACATCGTGGAGATG
  CAGCTGAGGCAGCACAAGGAGAGCCTCGTGGCAGGCCAGTGGAGGGACATGATGGACTACATGCTCCAAG
  GGGTGGCGCAGCCGAGCATGGAAGAGGGCTCTGGACAGCTCCTGGAAGGGCACGTGCACATGGCTGCAGT
  GGACCTCCTGATCGGTGGCACTGAGACCACAGCAAACACCCTCTCCTGGGCCGTGGTTTTTTTGCTTCAC
  CACCCTGAGATTCAGCAGCGACTGCAGGAGGAGCTAGACCACGAACTGGGCCCTGGTGCCTCCAGCTCCC
  GGGTCCCCTACAAGGACCGTGCACGGCTGCCCTTGCTCAATGCCACCATCGCCGAGGTGCTGCGCCTGCG
  GCCCGTTGTGCCCTTAGCCTTGCCCCACCGCACCACACGGCCCAGCAGCATCTCCGGCTACGACATCCCT
  GAGGGCACAGTCATCATTCCGAACCTCCAAGGCGCCCACCTGGATGAGACGGTCTGGGAGAGGCCACATG
  AGTTCTGGCCTGATCGCTTCCTGGAGCCAGGCAAGAACTCCAGAGCTCTGGCCTTCGGCTGCGGTGCCCG
  CGTGTGCCTGGGCGAGCCGCTGGCGCGCCTGGAGCTCTTCGTGGTGCTGACCCGACTGCTGCAGGCCTTC
  ACGCTGCTGCCCTCCGGGGACGCCCTGCCCTCCCTGCAGCCCCTGCCCCACTGCAGTGTCATCCTCAAGA
  TGCAGCCTTTCCAAGTGCGGCTGCAGCCCCGGGGGATGGGGGCCCACAGCCCGGGCCAGAGCCAGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001128590
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001128590.3, NP_001122062.3
• RefSeq Size: 2041 bp
• RefSeq ORF: 1398 bp
• Locus ID: 1589
• Cytogenetics: 6p21.33
• Protein Families: Druggable Genome, P450
• Protein Pathways: C21-Steroid hormone metabolism, Metabolic pathways
• Gene Summary: 'This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and hydroxylates steroids at the 21 position. Its activity is required for the synthesis of steroid hormones including cortisol and aldosterone. Mutations in this gene cause congenital adrenal hyperplasia. A related pseudogene is located near this gene; gene conversion events involving the functional gene and the pseudogene are thought to account for many cases of steroid 21-hydroxylase deficiency. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a.