LRRC32 (NM_001128922) Human Untagged Clone

CAT#: SC323169

LRRC32 (untagged)-Human leucine rich repeat containing 32 (LRRC32), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LRRC32
• Synonyms: D11S833E; GARP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001128922, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGACCCCAGATCCTGCTGCTCCTGGCCCTGCTGACCCTAGGCCTGGCTGCACAACACCAAGACAAAG
  TGCCCTGTAAGATGGTGGACAAGAAGGTCTCGTGCCAGGTTCTGGGCCTGCTCCAGGTCCCCTCGGTGCT
  CCCGCCAGACACTGAGACCCTTGATCTATCTGGGAACCAGCTGCGGAGTATCCTGGCCTCACCCCTGGGC
  TTCTACACAGCACTTCGTCACCTGGACCTGAGCACCAATGAGATCAGCTTCCTCCAGCCAGGAGCCTTCC
  AGGCCCTGACCCACCTGGAGCACCTCAGCCTGGCTCACAACCGGCTGGCGATGGCCACTGCGCTGAGTGC
  TGGTGGCCTGGGCCCCCTGCCACGCGTGACCTCCCTGGACCTGTCTGGGAACAGCCTGTACAGCGGCCTG
  CTGGAGCGGCTGCTGGGGGAGGCACCCAGCCTGCATACCCTCTCACTGGCGGAGAACAGTCTGACTCGCC
  TCACCCGCCACACCTTCCGGGACATGCCTGCGCTGGAGCAGCTTGACCTGCATAGCAACGTGCTGATGGA
  CATCGAGGATGGCGCCTTCGAGGGTCTGCCCCGCCTGACCCATCTCAACCTCTCCAGGAATTCCCTCACC
  TGCATCTCCGACTTCAGCCTCCAGCAGCTGCGGGTGCTAGACCTGAGCTGCAACAGCATCGAGGCCTTTC
  AGACGGCCTCCCAGCCCCAGGCTGAGTTCCAGCTCACCTGGCTTGACCTGCGGGAGAACAAACTGCTCCA
  TTTCCCCGACCTGGCCGCGCTCCCGAGACTCATCTACCTGAACTTGTCCAACAACCTCATCCGGCTCCCC
  ACAGGGCCACCCCAGGACAGCAAGGGCATCCACGCACCTTCCGAGGGCTGGTCAGCCCTGCCCCTCTCAG
  CCCCCAGCGGGAATGCCAGCGGCCGCCCCCTTTCCCAGCTCTTGAATCTGGATTTGAGCTACAATGAGAT
  TGAGCTCATCCCCGACAGCTTTCTTGAGCACCTGACCTCCCTGTGCTTCCTGAACCTCAGCAGAAACTGC
  TTGCGGACCTTTGAGGCCCGGCGCTTAGGCTCCCTGCCCTGCCTGATGCTCCTTGACTTAAGCCACAATG
  CCCTGGAGACACTGGAACTGGGCGCCAGAGCCCTGGGGTCTCTGCGGACGCTGCTCCTACAGGGCAATGC
  CCTGCGGGACCTGCCCCCATACACCTTTGCCAATCTGGCCAGCCTGCAGCGGCTCAACCTGCAGGGGAAC
  CGAGTCAGCCCCTGTGGGGGGCCAGATGAGCCTGGCCCCTCCGGCTGTGTGGCCTTCTCCGGCATCACCT
  CCCTCCGCAGCCTGAGCCTGGTGGATAATGAGATAGAGCTGCTCAGGGCAGGGGCCTTCCTCCACACCCC
  ACTGACTGAGCTGGACCTTTCTTCCAATCCTGGGCTGGAGGTGGCCACGGGGGCCTTGGGAGGCCTGGAG
  GCCTCCTTGGAGGTCCTGGCACTGCAGGGCAACGGGCTGATGGTCCTGCAGGTGGACCTGCCCTGCTTCA
  TCTGCCTCAAGCGGCTCAATCTTGCCGAGAACCGCCTGAGCCACCTTCCCGCCTGGACACAGGCTGTGTC
  ACTGGAGGTGCTGGACCTGCGAAACAACAGCTTCAGCCTCCTGCCAGGCAGTGCCATGGGTGGCCTGGAG
  ACCAGCCTCCGGCGCCTCTACCTGCAGGGGAATCCACTCAGCTGCTGCGGCAATGGCTGGCTGGCAGCCC
  AGCTGCACCAGGGCCGTGTGGACGTGGACGCCACCCAGGACCTGATCTGCCGCTTCAGCTCCCAGGAGGA
  GGTGTCCCTGAGCCACGTGCGTCCCGAGGACTGTGAGAAGGGGGGACTGAAGAACATCAACCTCATCATC
  ATCCTCACCTTCATACTGGTCTCTGCCATCCTCCTCACCACGCTGGCCGCCTGCTGCTGCGTCCGCCGGC
  AGAAGTTTAACCAACAGTATAAAGCCTAA
  
  
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001128922
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001128922.1, NP_001122394.1
• RefSeq Size: 4222 bp
• RefSeq ORF: 1989 bp
• Locus ID: 2615
• Cytogenetics: 11q13.5
• Protein Families: Transmembrane
• Gene Summary: 'This gene encodes a type I membrane protein which contains 20 leucine-rich repeats. Alterations in the chromosomal region 11q13-11q14 are involved in several pathologies. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1-4 all encode the same isoform (a).