CLK4 (NM_020666) Human Untagged Clone

CAT#: SC323347

CLK4 (untagged)-Kinase deficient mutant (K189M) of Human CDC-like kinase 4 (CLK4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CLK4
• Synonyms: DKFZp686A20267
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323347 sequence for NM_020666 edited (data generated by NextGen Sequencing)
  ATGCGGCATTCCAAAAGAACTCACTGTCCTGATTGGGATAGCAGAGAAAGCTGGGGACAT
  GAAAGCTATCGTGGAAGTCACAAGCGGAAGAGGAGATCTCATAGTAGCACACAAGAGAAC
  AGGCATTGTAAACCACATCACCAGTTTAAAGAATCTGATTGTCATTATTTAGAAGCAAGG
  TCCTTGAATGAGCGAGATTATCGGGACCGGAGATACGTTGACGAATACAGGAATGACTAC
  TGTGAAGGATATGTTCCTAGACATTATCACAGAGACATTGAAAGCGGGTATCGAATCCAC
  TGCAGTAAATCTTCAGTCCGCAGCAGGAGAAGCAGTCCTAAAAGGAAGCGCAATAGACAC
  TGTTCAAGTCATCAGTCACGTTCGAAGAGCCACCGAAGGAAAAGATCCAGGAGTATAGAG
  GATGATGAGGAGGGTCACCTGATCTGTCAAAGTGGAGACGTTCTAAGAGCAAGATATGAA
  ATCGTGGACACTTTGGGTGAAGGAGCCTTTGGCAAAGTTGTAGAGTGCATTGATCATGGC
  ATGGATGGCATGCATGTAGCAGTGATGATCGTAAAAAATGTAGGCCGTTACCGTGAAGCA
  GCTCGTTCAGAAATCCAAGTATTAGAGCACTTAAATAGTACTGATCCCAATAGTGTCTTC
  CGATGTGTCCAGATGCTAGAATGGTTTGATCATCATGGTCATGTTTGTATTGTGTTTGAA
  CTACTGGGACTTAGTACTTACGATTTCATTAAAGAAAACAGCTTTCTGCCATTTCAAATT
  GACCACATCAGGCAGATGGCGTATCAGATCTGCCAGTCAATAAATTTTTTACATCATAAT
  AAATTAACCCATACAGATCTGAAGCCTGAAAATATTTTGTTTGTGAAGTCTGACTATGTA
  GTCAAATATAATTCTAAAATGAAACGTGATGAACGCACACTGAAAAACACAGATATCAAA
  GTTGTTGACTTTGGAAGTGCAACGTATGATGATGAACATCACAGTACTTTGGTGTCTACC
  CGGCACTACAGAGCTCCCGAGGTCATTTTGGCTTTAGGTTGGTCTCAGCCTTGTGATGTT
  TGGAGCATAGGTTGCATTCTTATTGAATATTACCTTGGTTTCACAGTCTTTCAGACTCAT
  GATAGTAAAGAGCACCTGGCAATGATGGAACGAATATTAGGACCCATACCACAACACATG
  ATTCAGAAAACAAGAAAACGCAAGTATTTTCACCATAACCAGCTAGATTGGGATGAACAC
  AGTTCTGCTGGTAGATATGTTAGGAGACGCTGCAAACCGTTGAAGGAATTTATGCTTTGT
  CATGATGAAGAACATGAGAAACTGTTTGACCTGGTTCGAAGAATGTTAGAATATGATCCA
  ACTCAAAGAATTACCTTGGATGAAGCATTGCAGCATCCTTTCTTTGACTTATTAAAAAAG
  AAATGA
  
  Clone variation with respect to NM_020666.2
  566 a=>t;567 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_020666 unedited
  CCCGCCGTCTCAGCAATGGGCGGTAGGCGTGTACGGCTGTGGAGGTCTATATAAGCAGAGCTCGTTTAGT
  GAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGAAGACGGCAG
  TTTGGCGACATTTCTCGGCCGAAGGGCCATTTGCTTTTGCGGAGATGCGGCATTCCAAAAGAACTCACTG
  TCCTGATTGGGATAGCAGAGAAAGCTGGGGACATGAAAGCTATCGTGGAAGTCACAAGCGGAAGAGGAGA
  TCTCATAGTAGCACACAAGAGAAACAGGCATTTGTAAACCACATCACCAGTTTAAAGAAATCTGATTTGA
  TGTTCAATTGGGACTGTTTTACCCAGATTTGCTTTCTAAATAACCCAGCTGGGGGTAACTTTTAAAAAAC
  AGACCTGGGTGAATATAAAAAACGATGGCCCTAACCCTCATCGGTCTTGCCTATTGGAAAGGCTATATTG
  AAATATTAATTGGGCATTAATTGAAAATTTAAAAAAATTAATAATTCGGTCAATCCGGCTCACCCGGTCC
  ATAGCCGTGTGTAATTCTGTAAACTTTGTTTATATACTCTCTGACCCTGTGCTCATTGCCCTATACTAAT
  TGAAGACACTCCTACCCTTTAATTTGGCCCACGGGTTACACCCGTGTTGGACACTAAACGTGTTAGGGCA
  AACCTCTATTCTCCGCATTCCCCAGATGTGTGGCGATTTTTCAAAAGGTTGACTTATGAGAGAGCCTAAG
  ATGCGCCGACTATAGTATGGGTTCCTCGAGTTCGGAAGTCACTTACGACAGACCAGTACCAAATTCCGTG
  TTCCGAACCCTAGCAGTCTGAAAAAA
  
  Kinase domain sequence

  >SC323347 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  TGAMRTTCTAGAGCAGATATGAATCGTGGACACTTTGGGTGAAGGAGCCTTTGGCAAAGTTGTAGAGTGC
  ATTGATCATGGCATGGATGGCATGCATGTAGCAGTGATGATCGTAAAAAATGTAGGCCGTTACCGTGAAG
  CAGCTCGTTCAGAAATCCAAGTATTAGAGCACTTAAATAGTACTGATCCCAATAGTGTCTTCCGATGTGT
  CCAGATGCTAGAATGGTTTGATCATCATGGTCATGTTTGTATTGT
  
• Restriction Sites: Please inquire
• ACCN: NM_020666
• ORF Size: 1446 bp
• Insert Size: 2820
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_020666.2, NP_065717.1
• RefSeq Size: 2524
• RefSeq ORF: 1446
• Locus ID: 57396
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: The protein encoded by this gene belongs to the CDC2-like protein kinase (CLK) family. This protein kinase can interact with and phosphorylate the serine- and arginine-rich (SR) proteins, which are known to play an important role in the formation of spliceosomes, and thus may be involved in the regulation of alternative splicing. Studies in the Israeli sand rat Psammomys obesus suggested that the ubiquitin-like 5 (UBL5/BEACON), a highly conserved ubiquitin-like protein, may interact with and regulate the activity of this kinase. Multiple alternatively spliced transcript variants have been observed, but the full-length natures of which have not yet been determined. [provided by RefSeq, Jul 2008]