NEK9 (NM_033116) Human Untagged Clone

CAT#: SC323388

NEK9 (untagged)-Kinase deficient mutant (K81M) of Human NIMA (never in mitosis gene a)- related kinase 9 (NEK9)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NEK9
• Synonyms: APUG; LCCS10; NC; NERCC; NERCC1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323388 sequence for NM_033116 edited (data generated by NextGen Sequencing)
  ATGTCGGTGCTGGGCGAGTACGAGCGACACTGCGATTCCATCAACTCGGACTTTGGGAGC
  GAGTCCGGGGGTTGCGGGGACTCGAGTCCGGGGCCTAGCGCCAGTCAGGGGCCGCGAGCC
  GGCGGCGGCGCGGCGGAGCAGGAGGAACTGCACTACATCCCCATCCGCGTCCTGGGCCGC
  GGCGCCTTCGGGGAAGCCACGCTGTACCGCCGCACCGAGGATGACTCACTGGTTGTGTGG
  ATGGAAGTCGATTTGACCCGGCTGTCTGAGAAGGAACGTCGTGATGCCTTGAATGAGATT
  GTTATTCTGGCACTGCTGCAGCATGACAACATTATTGCCTACTACAATCACTTCATGGAC
  AATACCACGCTGCTGATTGAGCTGGAATATTGTAATGGAGGGAACCTGTATGACAAAATC
  CTTCGTCAGAAGGACAAGTTGTTTGAGGAAGAGATGGTGGTGTGGTACCTATTTCAGATT
  GTTTCAGCAGTGAGCTGCATCCATAAAGCTGGAATCCTTCATAGAGATATAAAGACATTA
  AATATTTTTCTGACCAAGGCAAACCTGATAAAACTTGGAGATTATGGCCTAGCAAAGAAA
  CTTAATTCTGAGTATTCCATGGCTGAGACGCTTGTGGGAACCCCATATTACATGTCTCCA
  GAGCTCTGTCAAGGAGTAAAGTACAATTTCAAGTCTGATATCTGGGCAGTTGGCTGCGTC
  ATTTTTGAACTGCTTACCTTAAAGAGGACGTTTGATGCTACAAACCCACTTAACCTGTGT
  GTGAAGATCGTGCAAGGAATTCGGGCCATGGAAGTTGACTCTAGCCAGTACTCTTTGGAA
  TTGATCCAAATGGTTCATTCGTGCCTTGACCAGGATCCTGAGCAGAGACCTACTGCAGAT
  GAACTTCTAGATCGCCCTCTTCTCAGGAAACGCAGGAGAGAGATGGAGGAAAAAGTCACT
  CTGCTTAATGCACCTACAAAGAGACCAAGGTCAAGCACTGTGACTGAAGCACCCATTGCT
  GTAGTAACATCACGAACCAGTGAAGTCTATGTTTGGGGTGGTGGAAAATCCACCCCCCAG
  AAACTGGATGTTATCAAGAGTGGCTGTAGTGCCCGGCAGGTCTGTGCAGGGAATACCCAC
  TTTGCTGTGGTCACAGTGGAGAAGGAACTGTACACTTGGGTGAACATGCAAGGAGGCACT
  AAACTCCATGGTCAGCTGGGCCATGGAGACAAAGCCTCCTATCGACAGCCAAAGCATGTG
  GAAAAGTTGCAAGGCAAAGCTATCCATCAGGTGTCATGTGGTGATGATTTCACTGTCTGT
  GTGACTGATGAGGGTCAGCTCTATGCCTTCGGATCAGATTATTATGGCTGCATGGGGGTG
  GACAAAGTTGCTGGCCCTGAAGTGCTAGAACCCATGCAGCTGAACTTCTTCCTCAGCAAT
  CCAGTGGAGCAGGTCTCCTGTGGAGATAATCATGTGGTGGTTCTGACACGAAACAAGGAA
  GTCTATTCTTGGGGCTGTGGCGAATATGGACGACTGGGTTTGGATTCAGAAGAGGATTAT
  TATACACCACAAAAGGTGGATGTTCCCAAGGCCTTGATTATTGTTGCAGTTCAATGTGGC
  TGTGATGGGACATTTCTGTTGACCCAGTCAGGCAAAGTGCTGGCCTGTGGACTCAATGAA
  TTCAATAAGCTGGGTCTGAATCAGTGCATGTCGGGAATTATCAACCATGAAGCATACCAT
  GAAGTTCCCTACACAACGTCCTTTACCTTGGCCAAACAGTTGTCCTTTTATAAGATCCGT
  ACCATTGCCCCAGGCAAGACTCACACAGCTGCTATTGATGAGCGAGGCCGGCTGCTGACC
  TTTGGCTGCAACAAGTGTGGGCAGCTGGGCGTTGGGAACTACAAGAAGCGTCTGGGAATC
  AACCTGTTGGGGGGACCCCTTGGTGGGAAGCAAGTGATCAGGGTCTCCTGCGGTGATGAG
  TTTACCATTGCTGCCACTGATGATAATCACATTTTTGCCTGGGGCAATGGTGGTAATGGC
  CGCCTGGCAATGACCCCCACAGAGAGACCACATGGCTCTGATATCTGTACCTCATGGCCT
  CGGCCTATTTTTGGATCTCTGCATCATGTCCCGGACCTGTCTTGCCGTGGATGGCATACC
  ATTCTCATCGTTGAGAAAGTATTGAATTCTAAGACCATCCGTTCCAATAGCAGTGGCTTA
  TCCATTGGAACTGTGTTTCAGAGCTCTAGCCCGGGAGGAGGCGGCGGGGGCGGCGGTGGT
  GAAGAAGAGGACAGTCAGCAGGAATCTGAAACTCCTGACCCAAGTGGAGGCTTCCGAGGA
  ACAATGGAAGCAGACCGAGGAATGGAAGGTTTAATCAGTCCCACAGAGGCCATGGGGAAC
  AGTAATGGGGCCAGCAGCTCCTGTCCTGGCTGGCTTCGAAAGGAGCTGGAAAATGCAGAA
  TTTATCCCCATGCCTGACAGCCCATCTCCTCTCAGTGCAGCGTTTTCAGAATCTGAGAAA
  GATACCCTGCCCTATGAAGAGCTGCAAGGACTCAAAGTGGCCTCTGAAGCTCCTTTGGAA
  CACAAACCCCAAGTAGAAGCCTCGTCACCTCGGCTGAATCCTGCAGTAACCTGTGCTGGG
  AAGGGAACACCACTGACTCCTCCTGCGTGTGCGTGCAGCTCTCTGCAGGTGGAGGTTGAG
  AGATTGCAGGGTCTGGTGTTAAAGTGTCTGGCTGAACAACAGAAGCTACAGCAAGAAAAC
  CTCCAGATTTTTACCCAACTGCAGAAGTTGAACAAGAAATTAGAAGGAGGGCAGCAGGTG
  GGGATGCATTCCAAAGGAACTCAGACAGCAAAGGAAGAGATGGAAATGGATCCAAAGCCT
  GACTTAGATTCAGATTCCTGGTGCCTCCTGGGAACAGACTCCTGTAGACCCAGCCTCTAG
  
  Clone variation with respect to NM_033116.4
  242 a=>t;324 c=>t;1286 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_033116 unedited
  CCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGGGCGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
  ACCGTCAAAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGACGGATCCGCGGG
  CCGGCATCTGAAGCGAGCGGGACGCAGCGCGGCCAGGGCCTCCGGGCATACGCAGGCTGGTCCCCAAGGC
  CCGCGGCCGCCGCCATGTCGGTGCTGGGCGAGTACGAGCGACACTGCGATTCCATCAACTCGGACTTTGG
  GAGCGAGTCCGGGGGTTGCGGGGACTCGAGTCCGGGGCCTAGCGCCAGTCAGGGGCCGCGATCTTGTTTG
  TTGGTGCGGCGGAGCAGGAGGAACTGCACTACATCCCCATCCCGCGTCCTGGGCCGCGGCGCCGTCGGGG
  AAGCCACCGCTTGTACCGGCCGCACCGAGGATGACTCACTGGTTGGTGTGGATGGAAGTCGATTTGACCC
  GGCTTGTCTGAGAGGAACGTCGTTGAGCCTTGATGAGATTGTTATTCCTGGCACTGCTGGAGCTGGCCAC
  ATATTGCTAACTACATCACTTCATGGCCATACCAGCGCTGTGGATGACCTGAAAATGTAATGGAGGAACC
  GTGTAGACAATCTCTTCTCAAGGACATTTGTTGAGAAAGGGTGTGGACCATTCAATGTTACACAGACTGT
  ACTAAAGCTGAATCTTAGGAGATCAGACCATACTTTCTGCACGGAAACGTAACTGGCATTGCTTACGGAT
  CATTTTCGATTTTTCTGCGAACCTTGGACCTTATATGTTCC
  
  Kinase domain sequence

  >SC323388 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CCCTGMGCAATGGGCGGTAGGCKGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA
  GAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGACGGATCCGCGGGCCGGCAT
  CTGAAGCGAGCGGGACGCAGCGCGGCCAGGGCCTCCGGGCATACGCAGGCTGGTCCCCAAGGCCCGCGGC
  CGCCGCCATGTCGGTGCTGGGCGAGTACGAGCGACACTGCGATTC
  
• Restriction Sites: Please inquire
• ACCN: NM_033116
• ORF Size: 2940 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_033116.3, NP_149107.3
• RefSeq Size: 3244
• RefSeq ORF: 2940
• Locus ID: 91754
• Domains: RCC1, pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein is activated in mitosis and, in turn, activates other family members during mitosis. This protein also mediates cellular processes that are essential for interphase progression. [provided by RefSeq, Jul 2016]