MAST3 (NM_015016) Human Untagged Clone

CAT#: SC323438

MAST3 (untagged)-Kinase deficient mutant (K226M) of Human microtubule associated serine/threonine kinase 3 (MAST3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAST3
• Synonyms: KIAA0561
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_015016, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGACGAGTCGAGCCTCCTGCGGCGCCGCGGGCTCCAGAAGGAGCTGAGCCTGCCACGCCGAGGACGTG
  GCTGCCGCAGCGGGAACCGCAAGAGCTTGGTGGTAGGAACGCCCTCCCCGACCCTCTCCCGGCCCCTGTC
  GCCATTGTCGGTCCCAACGGCAGGCAGCAGCCCCTTGGATAGTCCTCGGAATTTCTCGGCTGCCTCTGCC
  CTAAATTTCCCCTTTGCCCGGAGGGCAGACGGCAGAAGATGGTCCCTCGCGTCTCTCCCATCTTCCGGCT
  ATGGAACCAACACACCCAGCTCCACCCTCTCGTCAAGCTCATCCTCCCGGGAACGTCTCCACCAGCTTCC
  CTTCCAGCCGACGCCGGACGAGCTGCACTTCCTGTCCAAGCACTTCCGCAGCTCAGAGAATGTGCTTGAT
  GAGGAAGGCGGCCGGTCACCCCGCCTCCGACCCCGCTCTCGCAGTCTCAGCCCGGGCCGTGCAACGGGGA
  CCTTCGACAATGAGATTGTCATGATGAATCACGTGTACCGGGAGAGGTTCCCCAAGGCCACAGCACAGAT
  GGAGGGCCGTCTGCAGGAGTTCCTGACGGCCTACGCGCCCGGCGCCCGGCTGGCGCTGGCTGATGGCGTC
  TTGGGCTTCATCCACCACCAGATCGTCGAGCTGGCCCGAGACTGCTTGGCCAAGTCTGGCGAGAACCTCG
  TCACCTCCCGCTACTTCCTAGAGATGCAGGAGAAGCTGGAGCGGCTTCTGCAGGATGCCCATGAGCGTTC
  GGACAGTGAGGAGGTCAGCTTCATCGTCCAGCTTGTCCGGAAACTGCTGATCATCATCTCACGGCCAGCT
  CGGCTGCTGGAGTGTCTGGAGTTTGACCCTGAGGAATTTTACCACCTGCTGGAGGCGGCTGAGGGCCATG
  CGCGGGAGGGCCAAGGCATTAAGACTGACCTTCCACAGTACATCATTGGGCAGCTGGGCCTGGCCAAGGA
  CCCCCTGGAGGAGATGGTGCCACTGAGTCACCTCGAAGAAGAACAGCCCCCAGCACCTGAGTCCCCAGAG
  AGCCGCGCCCTGGTCGGCCAGTCACGGAGGAAGCCATGCGAAAGCGACTTTGAGACCATCAAACTCATTA
  GCAACGGAGCCTATGGGGCCGTCTACCTGGTGCGGCACCGTGACACACGGCAGCGCTTTGCCATCAAGAA
  GATCAACAAACAGAACTTGATCCTGCGTAACCAGATCCAGCAGGTCTTTGTGGAGCGTGACATTCTCACC
  TTTGCCGAGAACCCCTTTGTGGTCAGCATGTTCTGCTCCTTTGAGACCCGGCGCCACCTATGTATGGTCA
  TGGAATACGTGGAAGGCGGCGACTGCGCCACGCTCCTGAAGAACATGGGCCCGCTGCCCGTGGACATGGC
  CCGCCTGTACTTCGCCGAGACGGTGTTGGCGCTGGAGTACCTGCATAACTATGGCATCGTGCACCGTGAC
  CTCAAACCAGACAATCTGCTCATCACCTCGCTTGGCCACATCAAGCTCACGGACTTCGGCCTGTCCAAGA
  TCGGCCTCATGAGCATGGCCACCAACCTCTATGAGGGCCACATCGAGAAGGACGCCCGAGAGTTCATCGA
  CAAGCAGGTGTGTGGGACGCCGGAGTACATAGCCCCCGAGGTGATCTTCCGCCAGGGCTATGGGAAGCCA
  GTGGACTGGTGGGCCATGGGCGTCGTCCTCTATGAGTTTCTGGTGGGCTGCGTGCCTTTCTTTGGAGATA
  CCCCCGAGGAACTCTTCGGTCAGGTGGTCAGCGATGAGATCATGTGGCCAGAGGGAGATGAGGCCCTTCC
  AGCAGACGCCCAGGACCTCATCACCAGGTTGCTCCGGCAGAGCCCGCTGGACCGTCTGGGCACTGGTGGC
  ACCCACGAAGTGAAGCAGCACCCCTTTTTCCTGGCCCTGGACTGGGCAGGGCTTCTCCGACACAAAGCCG
  AGTTCGTGCCCCAGCTCGAAGCTGAGGATGATACCAGCTACTTTGACACACGTTCGGAACGTTACCGCCA
  TCTGGGCTCCGAGGACGACGAGACCAATGATGAAGAATCGTCCACAGAGATCCCCCAGTTCTCCTCCTGC
  TCCCACCGGTTCAGCAAGGTCTACAGCAGCTCTGAGTTCCTGGCCGTCCAGCCCACTCCTACCTTCGCTG
  AAAGGAGCTTCAGTGAAGACCGGGAGGAGGGGTGGGAGCGCAGCGAAGTGGACTATGGCCGCCGGCTGAG
  TGCTGACATCCGGCTGAGGTCCTGGACATCCTCTGGATCCTCCTGTCAGTCATCTTCGTCCCAGCCCGAG
  CGGGGTCCCAGCCCATCTCTCCTGAATACCATCAGCCTGGACACAATGCCCAAGTTTGCCTTCTCATCAG
  AGGATGAGGGGGTAGGCCCAGGCCCTGCAGGCCCCAAGAGGCCCGTCTTCATTCTAGGGGAGCCTGACCC
  CCCACCAGCGGCCACCCCAGTGATGCCCAAGCCCTCGAGCCTTTCTGCCGACACAGCTGCTCTCAGCCAC
  GCCCGCCTACGGAGCAATAGCATCGGCGCCCGACACTCCACACCAAGGCCTCTGGATGCCGGCCGGGGCC
  GCCGCCTTGGGGGCCCAAGAGACCCAGCCCCTGAGAAGTCCAGAGCCTCCTCCAGCGGTGGCAGTGGTGG
  CGGCAGTGGGGGCCGCGTGCCCAAGTCAGCCTCTGTCTCTGCCCTGTCCCTCATCATCACGGCAGATGAT
  GGCAGCGGCGGCCCCCTCATGAGCCCCCTTTCCCCGCGCTCTCTGTCCTCGAACCCGTCGTCCCGTGACT
  CTTCGCCGAGCCGAGACCCGTCCCCCGTGTGTGGCAGCCTGCGGCCCCCCATCGTTATCCACAGCTCTGG
  CAAGAAGTACGGCTTCAGCCTGCGGGCGATCCGCGTCTACATGGGTGATAGCGACGTCTACACTGTGCAC
  CACGTCGTCTGGAGTGTGGAGGACGGAAGCCCCGCCCAGGAGGCGGGCCTGCGGGCTGGGGACCTCATCA
  CCCACATCAACGGGGAGTCAGTGCTGGGGCTGGTGCACATGGACGTCGTGGAGCTGCTGCTGAAGAGCGG
  CAACAAGATATCCCTGCGGACCACAGCCCTGGAGAACACCTCCATCAAGGTGGGCCCCGCCCGGAAGAAT
  GTGGCCAAGGGCCGCATGGCACGCAGGAGCAAGAGGAGCCGTCGGCGGGAGACCCAGGATCGGCGGAAGT
  CACTTTTCAAGAAGATCTCCAAGCAGACCTCCGTGCTGCACACCAGCCGCAGCTTCTCCTCCGGACTCCA
  CCACTCACTGTCATCCAGTGAGAGCCTCCCCGGCTCGCCCACCCACAGCCTCTCCCCCAGCCCCACCACT
  CCCTGCCGAAGCCCAGCCCCTGATGTCCCAGCAGATACCACTGCATCCCCACCCAGCGCATCCCCGAGCT
  CCAGCAGCCCCGCCTCCCCAGCTGCTGCTGGCCACACCCGCCCCAGCTCCCTGCACGGCCTGGCTGCCAA
  GCTTGGGCCACCCCGCCCCAAGACTGGCCGCCGCAAGTCCACCAGCAGCATCCCGCCCTCCCCGCTGGCC
  TGCCCGCCCATCTCCGCGCCCCCACCCCGCTCGCCCTCGCCCCTGCCCGGGCACCCGCCCGCACCTGCCC
  GATCCCCGCGGCTGCGCCGGGGCCAGTCAGCTGACAAGCTGGGCACAGGGGAGCGGCTGGATGGGGAGGC
  GGGGCGGCGCACTCGTGGGCCAGAGGCCGAGCTCGTGGTCATGCGGCGGCTGCACCTGTCCGAGCGCCGA
  GACTCCTTCAAGAAGCAGGAGGCCGTGCAGGAGGTTAGCTTCGATGAGCCGCAGGAGGAGGCCACTGGGC
  TGCCCACCTCAGTGCCACAGATCGCCGTGGAGGGCGAGGAAGCCGTGCCAGTAGCTCTCGGGCCCACCGG
  AAGAGACTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_015016 unedited
  CCCGCCGTCTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCATTTAGGTG
  ACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGGCACGAGGACGAGTCGAGCC
  TCCTGCGGCGCCGCGGGGGCCAGAAGGAGCTGAGCCTGCCACGCCGAGGACGTGGCTGCCGCAGCGGGAA
  CCGCAAGAGCTTGGTGGTAGGAACGCCCTCCCCGACCCTCTCCCGGCCCCTGTCGCCATTGTCGGTCCCA
  ACGGCAGGCAGCAGCCCCCTTGGATAGTCCTCGGAATTTCTCGGGCTGCCTCTGCCCTAAATTTCCCCTT
  TGCCCGGAGGGCAGACGGCAGAAGATGGGTCCCTCGCGTCTCTCCAATCTTTCCGGCTATGGACCCAACA
  CACCCCAGCTTCCACCTTCTCGTCAGGCTCATCCTCCCGGGACCGTCTCCACCGCCTTCCCTTCCGGCCA
  ACCCCGGACAAGCTGCACTTCCTGTCCAAGCACTTCGGCAGTTCAGAAAATGTGCTTAATAAAAAAGGCC
  GCCCGGTCACCCCCGCCTCGAAACCGCGCTCGCAGATTCACAGCCCGGGGCCGGGGAAAGGGGAGACTCT
  GAGAATGAGGATAGTCTAGAAGAAAATCCTGTATCCGGGGAAGAGGTTCCCCAGGCCCACACACATAGAG
  AGGCCCCTCGGAGATTTCGAGGCGTCTACGCCCGGGCCCCGGTGTGCTGTTGCATGTGGCCTCGGGGTCT
  CCCACACATATCTAGATGTGCCAGAATGTTGTGCCAATTTGGGAGAAACCTGACTCCGCGATTCTCTAAA
  AGCCGGGAAACTGAGCGGCTTTTAGAGTGCCCGAAGACTCGACGAGGAGATCTAATCTGCACATTCGGAA
  GTGATATATATCACAGAACATGGGTAGAAGTCTCGAATGAATCGCGAGACTATGCTCTAGACGCACGA
  
  Kinase domain sequence

  >SC323438 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  TATCAACTCATTAGCACGGAGCCTATGGGGCCGTCTACCTGGTGCGGCACCGTGACACACGGCAGCGCTT
  TGCCATCATGAAGATCAACAAACAGAACTTGATCCTGCGTAACCAGATCCAGCAGGTCTTTGTGGAGCGT
  GACATTCTCACCTTTGCCGAGAACCCCTTTGTGGTCAGCATGTTCTGCTCCTTTGAGACCCGGCGCCACC
  TATGTATGGTCATGGAATACGTGGAAGGCGGCGACTGCGCCACGC
  
• Restriction Sites: Please inquire
• ACCN: NM_015016
• ORF Size: 3930 bp
• Insert Size: 6000
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone may be unstable or toxic at high copy number in common E. coli strain. We recommend using a lower copy number E. coli strain, such as CopyCutter strain (http://www.epibio.com/item.asp?ID=435) for transformation and plasmid preparation. Please be aware that the DNA yield could be low. Additional aliquots of this clone can be ordered from OriGene.

Reference Data

• RefSeq: NM_015016.1, NP_055831.1
• RefSeq Size: 5893
• RefSeq ORF: 3930
• Locus ID: 23031
• Protein Families: Druggable Genome, Protein Kinase