LOK (STK10) (NM_005990) Human Untagged Clone

CAT#: SC323467

STK10 (untagged)-Kinase deficient mutant (K65M) of Human serine/threonine kinase 10 (STK10)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: STK10
• Synonyms: LOK; PRO2729
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323467 sequence for NM_005990 edited (data generated by NextGen Sequencing)
  ATGGCTTTTGCCAATTTCCGCCGCATCCTGCGCCTGTCTACCTTCGAGAAGAGAAAGTCC
  CGCGAATATGAGCACGTCCGCCGCGACCTGGACCCCAACGAGGTGTGGGAGATCGTGGGC
  GAGCTGGGCGACGGCGCCTTCGGCAAGGTTTACAAGGCCAAGAATAAGGAGACGGGTGCT
  TTGGCTGCGGCCATGGTCATTGAAACCAAGAGTGAGGAGGAGCTGGAGGACTACATCGTG
  GAGATTGAGATCCTGGCCACCTGCGACCACCCCTACATTGTGAAGCTCCTGGGAGCCTAC
  TATCACGACGGGAAGCTGTGGATCATGATTGAGTTCTGTCCAGGGGGAGCCGTGGACGCC
  ATCATGCTGGAGCTGGACAGAGGCCTCACGGAGCCCCAGATACAGGTGGTTTGCCGCCAG
  ATGCTAGAAGCCCTCAACTTCCTGCACAGCAAGAGGATCATCCACCGAGATCTGAAAGCT
  GGCAACGTGCTGATGACCCTCGAGGGAGACATCAGGCTGGCTGACTTTGGTGTGTCTGCC
  AAGAATCTGAAGACTCTACAGAAACGAGATTCCTTCATCGGCACGCCTTACTGGATGGCC
  CCCGAGGTGGTCATGTGTGAGACCATGAAAGACACGCCCTACGACTACAAAGCCGACATC
  TGGTCCCTGGGCATCACGCTGATTGAGATGGCCCAGATCGAGCCGCCACACCACGAGCTC
  AACCCCATGCGGGTCCTGCTAAAGATCGCCAAGTCAGACCCTCCCACGCTGCTCACGCCC
  TCCAAGTGGTCTGTAGAGTTCCGTGACTTCCTGAAGATAGCCCTGGATAAGAACCCAGAA
  ACCCGACCCAGTGCCGCGCAGCTGCTGGAGCATCCCTTCGTCAGCAGCATCACCAGTAAC
  AAGGCTCTGCGGGAGCTGGTGGCTGAGGCCAAGGCCGAGGTGATGGAAGAGATCGAAGAC
  GGCCGGGATGAGGGGGAAGAGGAGGACGCCGTGGATGCCGCCTCCACCCTGGAGAACCAT
  ACTCAGAACTCCTCTGAGGTGAGTCCGCCAAGCCTCAATGCTGACAAGCCTCTCGAGGAG
  TCACCTTCCACCCCGCTGGCACCCAGCCAGTCTCAGGACAGTGTGAATGAGCCCTGCAGC
  CAGCCCTCTGGGGACAGATCCCTCCAAACCACCAGTCCCCCAGTCGTGGCCCCTGGAAAT
  GAGAACGGCCTGGCAGTGCCTGTGCCCCTGCGGAAGTCCCGACCCGTGTCAATGGATGCC
  AGAATTCAGGTAGCCCAGGAGAAGCAAGTTGCTGAGCAGGGTGGGGACCTCAGCCCAGCA
  GCCAACAGATCTCAAAAGGCCAGCCAGAGCCGGCCCAACAGCAGCGCCCTGGAGACCTTG
  GGTGGGGAGAAGCTGGCCAATGGCAGCCTGGAGCCACCTGCCCAGGCAGCTCCAGGGCCT
  TCCAAGAGGGACTCGGACTGCAGCAGCCTCTGCACCTCTGAGAGCATGGACTATGGTACC
  AATCTCTCCACTGACCTGTCGCTGAACAAAGAGATGGGCTCTCTGTCCATCAAGGACCCG
  AAACTGTACAAAAAAACCCTCAAGCGGACACGCAAATTTGTGGTGGATGGTGTGGAGGTG
  AGCATCACCACCTCCAAGATCATCAGCGAAGATGAGAAGAAGGATGAGGAGATGAGATTT
  CTCAGGCGCCAGGAACTCCGAGAGCTTCGGCTGCTCCAGAAAGAAGAGCATCGGAACCAG
  ACCCAGCTGAGTAACAAGCATGAGCTGCAGCTGGAGCAAATGCATAAACGTTTTGAACAG
  GAAATCAACGCCAAGAAGAAGTTCTTTGACACGGAATTAGAGAACCTGGAGCGTCAGCAA
  AAGCAGCAAGTGGAGAAGATGGAGCAAGACCATGCCGTGCGCCGCCGGGAGGAGGCCAGG
  CGGATCCGCCTGGAGCAGGATCGGGACTACACCAGGTTCCAAGAGCAGCTCAAACTGATG
  AAGAAAGAGGTGAAGAACGAGGTGGAGAAGCTCCCCCGACAGCAGCGGAAGGAAAGCATG
  AAGCAGAAGATGGAGGAGCACACGCAGAAAAAGCAGCTTCTTGACCGGGACTTTGTAGCC
  AAGCAGAAGGAGGACCTGGAGCTGGCCATGAAGAGGCTCACCACCGACAACAGGCGGGAG
  ATCTGTGACAAGGAGCGCGAGTGCCTCATGAAGAAGCAGGAGCTCCTTCGAGACCGGGAA
  GCAGCCCTGTGGGAGATGGAAGAGCACCAGCTGCAGGAGAGGCACCAGCTGGTGAAGCAG
  CAGCTCAAAGACCAGTACTTCCTCCAGCGGCACGAGCTGCTGCGCAAGCATGAGAAGGAG
  CGGGAGCAGATGCAGCGCTACAACCAGCGCATGATAGAGCAGCTGAAGGTGCGGCAGCAA
  CAGGAAAAGGCGCGGCTGCCCAAGATCCAGAGGAGTGAGGGCAAGACGCGCATGGCCATG
  TACAAGAAGAGCCTCCACATCAACGGCGGGGGCAGCGCAGCTGAGCAGCGTGAGAAGATC
  AAGCAGTTCTCCCAGCAGGAGGAGAAGAGGCAGAAGTCGGAGCGGCTGCAGCAACAGCAG
  AAACACGAGAACCAGATGCGGGACATGCTGGCGCAGTGTGAGAGCAACATGAGCGAGCTG
  CAGCAGCTGCAGAATGAAAAGTGCCACCTCCTGGTAGAGCACGAAACCCAGAAACTGAAG
  GCCCTGGATGAGAGCCATAACCAGAACCTGAAGGAATGGCGGGACAAGCTTCGGCCGCGC
  AAGAAGGCTCTGGAAGAGGATCTGAACCAGAAGAAGCGGGAGCAGGAGATGTTCTTCAAG
  CTGAGCGAGGAGGCGGAGTGCCCAAACCCCTCCACCCCAAGCAAGGCCGCCAAGTTCTTC
  CCCTACAGTTCTGCGGATGCTTCTTAA
  
  Clone variation with respect to NM_005990.3
  194 a=>t;195 a=>g;756 g=>a;783 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_005990 unedited
  ACCCCCCGTCCCGCAACGGGGCGGAGGCGTGTACGGAGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
  ACCGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCGAATCCGGCACGAGGCGGCACAAGGGAG
  GCGGCCCTGGGGACCTGACAGGGCGGGGGCGGGGGCGGGCACGCCGCCGCCTGCGCGGTTCCGGGTGCTC
  CCGGCGCGGCGCTGCCCAGGCACACTTGACCGAAGCCGGGCTGCGGGGAAAGGCAGGTCTGGGCAGAAGG
  CGGACACGCTTCTGCGGTCCGTGCTTGGGCGATAGTCCGGACACGAAGGCGAGGAGAGGCGTAAGACAAC
  CCAAGACTTTTCGCGATGGTAATAAAGGGAACTGCTGGATTGGTGGTAACAGACCGGAATAAGTAGGGCA
  GAATCCAAGCAAAATAGCCAAATCCGGAAAAAAAACTAACACCCCTTTAAAAATCCCGAAAACGTTAGGT
  CGTCCCCCGGCTATCTT
  
  Kinase domain sequence

  >SC323467 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CAATKCACCACGAGGTKGGGAGWCGTGGGCGAGCTGGGCGACGGCGCCTTCGGCAAGGTTTACAAGGCCA
  AGAATAAGGAGACGGGTGCTTTGGCTGCGGCCATGGTCATTGAAACCAAGAGTGAGGAGGAGCTGGAGGA
  CTACATCGTGGAGATTGAGATCCTGGCCACCTGCGACCACCCCTACATTGTGAAGCTCCTGGGAGCCTAC
  TATCACGACGGGAAGCTGTGGATCATGATTGAGTTCTGTCCAGGG
  
• Restriction Sites: Please inquire
• ACCN: NM_005990
• ORF Size: 2907 bp
• Insert Size: 4700
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_005990.2, NP_005981.2
• RefSeq Size: 4398
• RefSeq ORF: 2907
• Locus ID: 6793
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a member of the Ste20 family of serine/threonine protein kinases, and is similar to several known polo-like kinase kinases. The protein can associate with and phosphorylate polo-like kinase 1, and overexpression of a kinase-dead version of the protein interferes with normal cell cycle progression. The kinase can also negatively regulate interleukin 2 expression in T-cells via the mitogen activated protein kinase kinase 1 pathway. [provided by RefSeq, Jul 2008]