EIF2AK1 (NM_014413) Human Untagged Clone

CAT#: SC323477

EIF2AK1 (untagged)-Kinase deficient mutant (K210M) of Human eukaryotic translation initiation factor 2-alpha kinase 1 (EIF2AK1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EIF2AK1
• Synonyms: HCR; HRI
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323477 sequence for NM_014413 edited (data generated by NextGen Sequencing)
  ATGCAGGGGGGCAACTCCGGGGTCCGCAAGCGCGAAGAGGAGGGCGACGGGGCTGGGGCT
  GTGGCTGCGCCGCCGGCCATCGACTTTCCCGCCGAGGGCCCGGACCCCGAATATGACGAA
  TCTGATGTTCCAGCAGAAATCCAGGTGTTAAAAGAACCCCTACAACAGCCAACCTTCCCT
  TTTGCAGTTGCAAACCAACTCTTGCTGGTTTCTTTGCTGGAGCACTTGAGCCACGTGCAT
  GAACCAAACCCACTTCGTTCAAGACAGGTGTTTAAGCTACTTTGCCAGACGTTTATCAAA
  ATGGGGCTGCTGTCTTCTTTCACTTGTAGTGACGAGTTTAGCTCATTGAGACTACATCAC
  AACAGAGCTATTACTCACTTAATGAGGTCTGCTAAAGAGAGAGTTCGTCAGGATCCTTGT
  GAGGATATTTCTCGTATCCAGAAAATCAGATCAAGGGAAGTAGCCTTGGAAGCACAAACT
  TCACGTTACTTAAATGAATTTGAAGAACTTGCCATCTTAGGAAAAGGTGGATACGGAAGA
  GTATACAAGGTCAGGAATAAATTAGATGGTCAGTATTATGCAATAAAAAAAATCCTGATT
  AAGGGTGCAACTAAAACAGTTTGCATGATGGTCCTACGGGAAGTGAAGGTGCTGGCAGGT
  CTTCAGCACCCCAATATTGTTGGCTATCACACCGCGTGGATAGAACATGTTCATGTGATT
  CAGCCACGAGCAGACAGAGCTGCCATTGAGTTGCCATCTCTGGAAGTGCTCTCCGACCAG
  GAAGAGGACAGAGAGCAATGTGGTGTTAAAAATGATGAAAGTAGCAGCTCATCCATTATC
  TTTGCTGAGCCCACCCCAGAAAAAGAAAAACGCTTTGGAGAATCTGACACTGAAAATCAG
  AATAACAAGTCGGTGAAGTACACCACCAATTTAGTCATAAGAGAATCTGGTGAACTTGAG
  TCGACCCTGGAGCTCCAGGAAAATGGCTTGGCTGGTTTGTCTGCCAGTTCAATTGTGGAA
  CAGCAGCTGCCACTCAGGCGTAATTCCCACCTAGAGGAGAGTTTCACATCCACCGAAGAA
  TCTTCCGAAGAAAATGTCAACTTTTTGGGTCAGACAGAGGCACAGTACCACCTGATGCTG
  CACATCCAGATGCAGCTGTGTGAGCTCTCGCTGTGGGATTGGATAGTCGAGAGAAACAAG
  CGGGGCCGGGAGTATGTGGACGAGTCTGCCTGTCCTTATGTTATGGCCAATGTTGCAACA
  AAAATTTTTCAAGAATTGGTAGAAGGTGTGTTTTACATACATAACATGGGAATTGTGCAC
  CGAGATCTGAAGCCAAGAAATATTTTTCTTCATGGCCCTGATCAGCAAGTAAAAATAGGA
  GACTTTGGTCTGGCCTGCACAGACATCCTACAGAAGAACACAGACTGGACCAACAGAAAC
  GGGAAGAGAACACCAACACATACGTCCAGAGTGGGTACTTGTCTGTACGCTTCACCCGAA
  CAGTTGGAAGGATCTGAGTATGATGCCAAGTCAGATATGTACAGCTTGGGTGTGGTCCTG
  CTAGAGCTCTTTCAGCCGTTTGGAACAGAAATGGAGCGAGCAGAAGTTCTAACAGGTTTA
  AGAACTGGTCAGTTGCCGGAATCCCTCCGTAAAAGGTGTCCAGTGCAAGCCAAGTATATC
  CAGCACTTAACGAGAAGGAACTCATCGCAGAGACCATCTGCCATTCAGCTGCTGCAGAGT
  GAACTTTTCCAAAATTCTGGAAATGTTAACCTCACCCTACAGATGAAGATAATAGAGCAA
  GAAAAAGAAATTGCAGAACTAAAGAAGCAGCTAAACCTCCTTTCTCAAGACAAAGGGGTG
  AGGGATGACGGAAAGGATGGGGGCGTGGGATGA
  
  Clone variation with respect to NM_014413.3
  629 a=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_014413 unedited
  CCCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCTAGCTGCAGCAT
  CGGAGTGTGGCAGTGCTGGGCTGGCCGGCGGGCTGGGCTGCGGCCCGCGCGCGGCCGGCGATGCAGGGGG
  GCAACTCCGGGGTCCGCAAGCGCGAAGAGGAGGGCGACGGGGCTGGGGCTGTGGCTGCGCCGCCGGCCAT
  CGACTTTCCCGCCGAGGGCCCGGACCCCGAATATGACGAATCTGATGTTCCAGCAGAAATCCAGGTGTGA
  AAAGAACCCCTAACAACAGCCAACCTTCCCTTTTGCAGTTGCAAACCACTCTTGCTGTTTTCTTTGCTGG
  GAGCACTTGAGCCACGTGCATGACCAAACCCACTTTCGTTTCAGACAGGGGTTTAAAGCTACTTTGCAAG
  ACGTTTTTCAAAATGGGGCTGGCGGTCCTCTTCACTTTAGGGCCGAGTTTAGCCTATTGGACCCACTACA
  CACGAACCTTACTACCTAATGAGGTCGGCTAAGAGAAGTCGTCGGATCTGTGAGATTCCCGATCGGAATC
  CATCAGGAATACCTGGACCAACTTCGTTCTAAGAATGGAACTGCTCTGGGGAGGTGACGGGAGATCCTCC
  GAAATTAGGCGTATAGCCTAGAACCGTAGTGGCAAACGTTGCAAAGTCCACGAACTTTCGGGCTTTCACA
  AAAATGTCAAACGACGACGGATCGTATAACCGAGCGCTTAC
  
  Kinase domain sequence

  >SC323477 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  TAYYMTCRGGAAWAATTAGTGWCAGTATTATGCAATAAAWAAAATCCTGATTAAGGGTGCAACTAAAACA
  GTTTGCATGATGGTCCTACGGGAAGTGAAGGGKGCAACTAAAACAGTTTGCATGATGGTCCTACGGGAAG
  TGAAGAATCCTGATTAAGGGTGCAACTAAAACAGTTTGCATGATGGTCCTACGGGAAGTGAAGAATCCTG
  ATTAAGGGTGCAACTAAAACAGTTTGCATGATGGTCCTACGGGAA
  
• Restriction Sites: Please inquire
• ACCN: NM_014413
• ORF Size: 1893 bp
• Insert Size: 2240
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_014413.2, NP_055228.2
• RefSeq Size: 2813
• RefSeq ORF: 1893
• Locus ID: 27102
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: The protein encoded by this gene acts at the level of translation initiation to downregulate protein synthesis in response to stress. The encoded protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.