ALPK2 (NM_052947) Human Untagged Clone

CAT#: SC323571

ALPK2 (untagged)-Kinase deficient mutant (K1718M) of Human alpha-kinase 2 (ALPK2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ALPK2
• Synonyms: HAK
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_052947, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAAGACTCCGAAGGGCCCCAGAGGCCCCCGCTGTGTTTTTTATCTACATTGCTTTCCCAGAAGGTTC
  CTGAGAAGTCAGACGCTGTGCTTCGCTGCATAATATCTGGTCAGCCCAAGCCAGAGGTAACTTGGTATAA
  GAATGGTCAGGCCATCGATGGGAGTGGCATTATTTCCAACTATGAATTCTTTGAGAATCAGTATATTCAT
  GTGTTACATCTCTCTTGCTGTACCAAAAATGATGCTGCTGTCTATCAAATCTCGGCTAAAAACTCTTTTG
  GAATGATCTGTTGTTCTGCTTCCGTTGAGGTTGAGTGCTCATCAGAGAACCCACAATTGTCTCCTAACCT
  GGAAGATGACAGGGACAGGGGTTGGAAACATGAAACAGGGACACATGAAGAAGAAAGGGCAAATCAGATT
  GATGAGAAGGAACATCCTTATAAGGAAGAAGAAAGCATCTCCCCGGGCACTCCCAGGTCAGCTGACTCCT
  CCCCCTCCAAATCCAACCATTCACTCTCCCTCCAGTCATTGGGCAATCTTGACATTAGTGTGTCCAGTTC
  TGAAAATCCTTTGGGTGTTAAAGGAACAAGGCACACTGGAGAGGCTTATGATCCAAGTAACACAGAAGAA
  ATTGCAAATGGGTTGCTTTTTCTTAATTCAAGTCATATTTATGAAAAACAAGACAGATGTTGCCACAAGA
  CAGTGCATTCCATGGCATCAAAGTTCACGGATGGTGACCTGAACAATGATGGTCCTCATGATGAAGGCTT
  ACGCTCTAGTCAGCAAAATCCCAAAGTACAGAAATACATTAGCTTCAGCCTCCCGCTATCTGAGGCAACT
  GCACACATTTACCCAGGTGACAGTGCCGTGGCCAACAAACAACCCAGCCCACAGCTTTCCAGTGAAGACT
  CTGACAGTGACTATGAACTTTGCCCAGAGATAACCCTAACCTACACCGAGGAGTTTTCAGATGATGACCT
  GGAGTATCTGGAATGTTCTGATGTTATGACGGATTACTCTAATGCAGTTTGGCAAAGGAACCTGCTGGGG
  ACTGAGCATGTTTTTTTATTAGAAAGCGATGACGAAGAGATGGAATTCGGTGAGCATTGCCTGGGTGGGT
  GTGAGCATTTCCTCAGTGGAATGGGTTGTGGGTCTCGGGTGTCGGGTGACGCTGGGCCTATGGTTGCCAC
  TGCTGGCTTCTGTGGTCATCACTCACAACCCCAAGAAGTTGGGGTGAGGAGCAGCAGAGTCTCCAAGCAC
  GGTCCCTCATCCCCACAAACAGGGATGACTCTCATTTTGGGACCTCACCAGGATGGAACGTCTTCAGTGA
  CAGAACAGGGGAGATATAAACTCCCCACTGCTCCCGAGGCTGCTGAAAATGATTATCCAGGAATTCAAGG
  AGAAACCAGAGACAGCCACCAAGCAAGAGAGGAATTTGCCAGTGACAATCTGCTCAACATGGATGAATCA
  GTAAGAGAGACAGAGATGAAGCTCTTGTCTGGTGAGTCAGAAAACTCAGGGATGAGCCAGTGTTGGGAGA
  CGGCAGCTGACAAGAGAGTGGGGGGAAAGGACTTATGGAGCAAGAGGGGTTCAAGGAAATCTGCCAGGGT
  GAGGCAGCCGGGAATGAAGGGAAATCCCAAGAAGCCGAATGCCAACCTGAGAGAAAGTACAACAGAAGGT
  ACCCTTCATCTCTGCTCTGCCAAAGAATCTGCTGAGCCCCCACTAACCCAGAGTGATAAAAGAGAGACTT
  CTCACACCACAGCAGCAGCGACTGGTCGGAGTTCCCATGCTGATGCAAGAGAATGTGCTATTTCAACCCA
  GGCAGAGCAAGAAGCAAAAACCCTTCAAACTTCAACAGACTCAGTCTCCAAAGAAGGCAACACAAATTGC
  AAGGGAGAAGGCATGCAAGTTAATACTCTATTTGAAACAAGCCAGGTTCCAGACTGGAGTGATCCTCCTC
  AGGTACAAGTTCAGGAAACAGTCAGAGAGACAATCTCTTGCAGCCAGATGCCAGCTTTCTCAGAGCCTGC
  TGGGGAGGAGTCCCCATTCACTGGGACCACAACAATTTCCTTCTCAAACTTAGGAGGGGTCCACAAGGAA
  AATGCATCATTAGCTCAACACTCGGAGGTCAAACCCTGTACCTGTGGTCCACAGCATGAAGAAAAACAAG
  ACAGAGATGGCAACATACCTGACAATTTCAGGGAAGACCTAAAATATGAGCAGAGCATCTCAGAAGCCAA
  TGATGAGACTATGTCCCCAGGTGTGTTCTCAAGGCATCTCCCCAAGGATGCTCGTGCTGACTTCAGGGAG
  CCTGTGGCTGTCTCTGTTGCTTCCCCTGAACCCACAGATACTGCCCTCACCCTGGAAAATGTGTGTGATG
  AGCCAAGGGACAGAGAAGCAGTGTGTGCAATGGAGTGTTTTGAGGCTGGTGACCAAGGAACGTGTTTTGA
  TACCATAGATTCTCTTGTTGGGAGACCAGTTGATAAATATTCGCCTCAAGAAATTTGCTCTGTAGATACG
  GAACTGGCAGAAGGTCAAAACAAAGTATCTGATTTATGTTCTTCTAATGACAAGACACTGGAAGTCTTTT
  TTCAGACACAAGTGTCTGAGACTTCAGTGTCTACGTGCAAAAGCAGCAAGGACGGCAACTCAGTCATGTC
  CCCTCTTTTTACCAGTACTTTCACCTTGAACATTTCACACACAGCTAGTGAAGGTGCCACAGGAGAAAAT
  CTAGCCAAGGTGGAGAATTCCACCTACCCACTGGCCTCCACAGTACATGCTGGCCAGGAGCAGCCAAGCC
  CCAGCAACTCAGGAGGGCTTGATGAAACACAGCTCCTTTCTTCTGAGAACAATCCTTTAGTGCAATTTAA
  AGAAGGAGGTGACAAGAGCCCCAGTCCTAGTGCCGCAGACACCACAGCCACACCAGCCAGTTATAGTTCA
  ATTGTGAGTTTTCCTTGGGAGAAGCCAACAACATTAACTGCTAATAATGAGTGCTTTCAAGCGACCAGGG
  AGACTGAGGACACATCAACTGTTACCATTGCCACCGAAGTCCACCCAGCCAAATACCTTGCTGTGTCAAT
  TCCTGAGGACAAGCATGCAGGTGGCACTGAGGAGAGGTTCCCTCGTGCATCCCATGAAAAGGTTTCCCAA
  TTTCCTTCCCAAGTGCAGTTGGATCATATTTTAAGTGGTGCTACCATCAAATCTACAAAAGAGCTACTTT
  GCAGGGCACCCAGTGTGCCAGGAGTCCCACACCATGTCCTGCAGCTCCCAGAGGGAGAGGGTTTCTGCAG
  TAATTCCCCTCTTCAGGTTGATAACCTGTCTGGAGATAAGAGCCAGACTGTGGACAGAGCAGACTTTAGG
  AGCTATGAAGAGAATTTCCAAGAAAGAGGAAGTGAAACAAAGCAGGGGGTCCAGCAGCAGAGCCTGTCCC
  AGCAGGGTTCTCTTTCTGCACCTGATTTCCAACAAAGTTTGCCTACGACATCTGCTGCACAAGAGGAAAG
  AAACTTGGTGCCCACGGCCCACTCACCCGCAAGCTCTAGGGAAGGAGCAGGGCAGCGCTCAGGTTGGGGG
  ACGAGGGTCTCCGTGGTGGCTGAAACTGCTGGGGAAGAAGACAGTCAGGCTCTGAGCAACGTTCCATCTC
  TCTCTGATATCCTTTTGGAAGAGTCTAAAGAATATAGACCTGGAAATTGGGAGGCAGGCAACAAGCTGAA
  GATTATAACTCTAGAGGCTTCCGCTTCTGAAATCTGGCCACCACGACAACTGACAAATTCTGAGAGCAAG
  GCATCAGACGGTGGTCTCATAATTCCTGACAAGGTCTGGGCTGTACCTGATAGTCTAAAGGCAGATGCTG
  TTGTGCCTGAATTGGCCCCCTCTGAAATAGCAGCATTGGCTCACAGTCCAGAGGATGCTGAGTCAGCCCT
  TGCTGATAGCAGAGAAAGCCATAAAGGCGAAGAGCCCACCATCAGTGTACATTGGAGAAGTCTTTCTTCC
  CGGGGTTTCAGCCAACCCAGACTCCTGGAGTCATCCGTGGACCCTGTAGATGAAAAGGAGTTATCTGTCA
  CAGATTCACTGTCAGCGGCTTCTGAAACTGGAGGGAAGGAAAATGTTAACAATGTGAGTCAAGACCAGGA
  GGAAAAACAACTCAAGATGGATCACACTGCCTTCTTTAAAAAGTTTCTGACCTGCCCTAAAATCCTAGAG
  TCCTCTGTAGATCCCATTGATGAGATAAGTGTGATAGAGTACACCAGGGCTGGAAAACCAGAGCCCTCTG
  AAACCACACCACAGGGCGCCAGAGAAGGGGGTCAATCAAATGACGGAAACATGGGCCACGAAGCGGAAAT
  CCAGCCGGCCATTTTGCAAGTTCCATGTCTCCAGGGAACCATTCTGAGTGAAAATAGAATCAGCAGAAGC
  CAAGAAGGCAGTATGAAGCAGGAGGCTGAACAAATTCAACCTGAGGAGGCAAAAACTGCCATTTGGCAAG
  TCCTGCAACCCAGCGAAGGCGGTGAAAGAATTCCAAGTGGATGTAGCATAGGCCAAATACAAGAAAGCAG
  TGATGGGAGCTTAGGGGAGGCTGAGCAAAGCAAAAAGGACAAAGCAGAATTGATTTCCCCCACTTCACCT
  CTTTCTAGTTGTCTTCCAATAATGACTCACGCTTCTCTTGGGGTTGACACGCACAACTCCACAGGCCAAA
  TTCATGACGTCCCTGAAAATGACATAGTTGAGCCCAGAAAGCGTCAGTATGTGTTTCCTGTTTCACAGAA
  AAGGGGAACTATTGAGAATGAGCGTGGGAAACCTTTGCCCTCTTCTCCTGATCTTACCAGGTTCCCTTGT
  ACTTCATCTCCTGAAGGAAATGTCACAGACTTTTTGATAAGCCACAAAATGGAGGAACCTAAAATAGAGG
  TGCTTCAAATTGGGGAAACCAAACCCCCAAGCTCATCTAGCTCCTCAGCGAAGACCTTGGCATTTATTTC
  AGGAGAACGTGAGTTAGAGAAAGCCCCTAAATTACTGCAGGATCCATGTCAAAAGGGCACCCTGGGCTGT
  GCGAAAAAGTCCAGGGAGAGAGAGAAGTCCCTGGAAGCCCGAGCAGGCAAATCGCCAGGGACCCTCACAG
  CAGTGACGGGGTCAGAGGAGGTCAAGAGGAAGCCAGAAGCCCCAGGCAGTGGACATTTAGCTGAGGGAGT
  AAAGAAGAAAATTTTGTCCAGGGTGGCAGCACTGAGGCTGAAACTGGAAGAAAAGGAAAATATCAGAAAG
  AACTCAGCCTTTCTTAAAAAGATGCCCAAACTCGAAACATCATTATCACACACAGAAGAGAAACAAGACC
  CAAAAAAGCCATCTTGCAAAAGAGAAGGAAGAGCTCCAGTATTACTGAAAAAGATCCAAGCTGAGATGTT
  CCCTGAACACTCTGGAAATGTAAAATTAAGCTGCCAATTTGCAGAAATTCATGAAGATTCTACTATCTGC
  TGGACAAAAGATTCAAAGTCCATAGCCCAAGTGCAGAGAAGTGCAGGGGACAACTCCACTGTTTCCTTTG
  CCATCGTGCAAGCCAGTCCGAAGGACCAGGGACTCTATTACTGCTGCATCAAGAACAGCTACGGAAAAGT
  GACTGCTGAATTTAACCTCACAGCTGAAGTTCTCAAACAGCTGTCAAGTCGCCAGGATACTAAAGGATGT
  GAAGAGATTGAATTCAGCCAACTCATCTTCAAAGAAGACTTCCTCCATGACAGCTACTTTGGGGGCCGCC
  TGCGTGGTCAGATCGCCACGGAGGAGCTGCACTTTGGAGAAGGGGTTCACCGCAAAGCCTTCCGCAGCAC
  AGTGATGCACGGCCTCATGCCTGTCTTCAAACCTGGCCATGCCTGTGTGCTTAAGGTGCACAATGCCATT
  GCCTATGGGACCAGAAATAATGATGAGCTCATCCAAAGGAACTACAAACTCGCTGCCCAGGAATGCTATG
  TTCAAAATACTGCCAGGTATTATGCCAAGATCTACGCTGCTGAAGCACAGCCTCTGGAAGGCTTTGGAGA
  AGTACCTGAGATCATTCCTATTTTTCTTATCCATCGGCCTGAGAACAATATCCCGTATGCTACAGTGGAG
  GAGGAGCTGATTGGAGAATTTGTGAAGTATTCCATCAGGGATGGGAAAGAAATAAACTTCTTGAGAAGAG
  AATCAGAAGCTGGTCAGAAATGTTGCACCTTCCAGCACTGGGTGTACCAGAAAACAAGTGGCTGCCTCCT
  GGTGACGGACATGCAAGGTGTAGGAATGAAGCTAACTGACGTTGGCATAGCAACGCTGGCTAAAGGGTAC
  AAGGGATTTAAAGGCAACTGTTCCATGACCTTCATTGATCAGTTTAAAGCACTACACCAGTGTAACAAGT
  ATTGCAAAATGCTGGGACTGAAATCCCTTCAAAACAACAACCAGAAACAGAAGCAGCCGAGCATTGGGAA
  AAGCAAAGTTCAAACAAACTCTATGACAATAAAGAAGGCAGGGCCTGAGACCCCAGGCGAAAAGAAAACC
  TAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_052947 unedited
  ACGGCCCCGTTGAAGCAATGGGCGGTAGGCGTGTACGGGGGGAGGTCTATATAAGCAGAGCTCGTTTAGT
  GAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGCCGGGAATGAA
  GGGAAATCCCAAGAAGCCGAATGCCAACCTGAGAGAAAGTACAACAGAAGGTACCCTTCATCTCTGCTCT
  GCCAAAGAATCTGCTGAGCCCCCACTAACCCAGAGTGATAAAAGAGAGACTTCTCACACCACAGCAGCAG
  CGACTGGTCGGAGTTCCCATGCTGATGCAAAGAGAATGTGCTATTTCAACCCAGGCAGAGCAAGAGGCAA
  AAACCCTTCAAACTTTCAACAGACTCCAGTCCTCAAAAAGGGCACCACAAATTGCAGGGAAAAAGGCATG
  CAAGTTATTCCTCTATTGGAAACAAGCCAGGTTCCAGACGGAATGGATCCTCCTCAGGTACAGGTTCAGG
  AACAGTTCAAAAAAACAATCTCTTGCAGCCGGATGCCACCTTTTCAAAACCCTGGTGGGGAGAATTCCCC
  TTTCATGGAACCCAACAATTTCCTTTCTAAACTTAGAAAGGGGCCCACGGAAAAATGCTATATACCTCAC
  ACTCAGGGTCACACTGTGCCCGTTGGGCCCAGATGTGAAAATCACACACAGAGTGGCATACCCGGTAAAT
  TTCGGGGAACACTATATGTGCGCAGAGCCTCTAGACCCTATGAGACACTGTCCCCCCGTGTGTCTAGCGC
  TCTCACAAAGCTCTCTGATCATGAGAACTTGTGTCTCTTGTTCCCGGAACCAAATAGCTGCCTTCTGAGA
  TGTGTAGACACGAGTCACACCGTGCTCATGTGTGTATGCGTACAGACAGGTGTTACTCACATCCTGTGAC
  CTTAGATATCCCACATCTCCTAGGCATTGCAGAAGGG
  
  Kinase domain sequence

  >SC323571 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  TCRGCAGRCTGACTTTGGAGAGGGGTTCACCGCAAGCCTTCCGCAGCACAGTGATGCACGGCCTCATGCC
  TGTCTTCAAACCTGGCCATGCCTGTGTGCTTATGGTGCACAATGCCATTGCCTATGGGACCAGAAATAAT
  GATGAGCTCATCCAAAGGAACTACAAACTCGCTGCCCAGGAATGCTATGTTCAAAATACTGCCAGGTATT
  ATGCCAAGATCTACGCTGCTGAAGCACAGCCTCTGGAAGGCTTTG
  
• Restriction Sites: Please inquire
• ACCN: NM_052947
• ORF Size: 5802 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_052947.2, NP_443179.2
• RefSeq Size: 6496
• RefSeq ORF: 5802
• Locus ID: 115701
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: Kinase that recognizes phosphorylation sites in which the surrounding peptides have an alpha-helical conformation. [UniProtKB/Swiss-Prot Function]