PRPK (TP53RK) (NM_033550) Human Untagged Clone

CAT#: SC323584

TP53RK (untagged)-Kinase deficient mutant (D162A) of Human TP53 regulating kinase (TP53RK)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TP53RK
• Synonyms: BUD32; C20orf64; dJ101A2; GAMOS4; Nori-2; Nori-2p; PRPK
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323584 sequence for NM_033550 edited (data generated by NextGen Sequencing)
  ATGGCGGCGGCCAGAGCTACTACGCCGGCCGATGGCGAGGAGCCCGCCCCGGAGGCTGAG
  GCTCTGGCCGCAGCCCGGGAGCGGAGCAGCCGCTTCTTGAGCGGCCTGGAGCTGGTGAAG
  CAGGGTGCCGAGGCGCGCGTGTTCCGTGGCCGCTTCCAGGGCCGCGCGGCGGTGATCAAG
  CACCGCTTCCCCAAGGGCTACCGGCACCCGGCGCTGGAGGCGCGGCTTGGCAGACGGCGG
  ACGGTGCAGGAGGCCCGGGCGCTCCTCCGCTGTCGCCGCGCTGGAATATCTGCCCCAGTT
  GTCTTTTTTGTGGACTATGCTTCCAACTGCTTATATATGGAAGAAATTGAAGGCTCAGTG
  ACTGTTCGAGATTATATTCAGTCCACTATGGAGACTGAAAAAACTCCCCAGGGTCTCTCC
  AACTTAGCCAAGACAATTGGGCAGGTTTTGGCTCGAATGCACGATGAAGACCTCATTCAT
  GGTGCCCTCACCACCTCCAACATGCTCCTGAAACCCCCCCTGGAACAGCTGAACATTGTG
  CTCATAGACTTTGGGCTGAGTTTCATTTCAGCACTTCCAGAGGATAAGGGAGTAGACCTC
  TATGTCCTGGAGAAGGCCTTCCTCAGTACCCATCCCAACACTGAAACTGTGTTTGAAGCC
  TTTCTGAAGAGCTACTCCACCTCCTCCAAAAAGGCCAGGCCAGTGCTAAAAAAATTAGAT
  GAAGTGCGCCTGAGAGGAAGAAAGAGGTCCATGGTTGGGTAG
  
  Clone variation with respect to NM_033550.3
  485 a=>c;486 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_033550 unedited
  ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTAACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGATCGGTTGGAG
  CTGTTGCGCCGAGCAGTCATGGCGGCGGCCAGAGCTACTACGCCGGCCGATGGCGAGGAGCCCGCCCCGG
  AGGCTGAGGCTCTGGCCGCAGCCCGGGAGCGGAGCAGCCGCTTCTTGAGCGGCCTGGAGCTGGTGAAGCA
  GGGTGCCGAGGCGCGCGTGTTCCGTGGCCGCTTCCAGGGCCGCGCGGCGGTGATCAAGCACCGCTTCCCC
  AAGGGCTACCGGCACCCGGCGCTGGAGGCGCGGCTTGGCAGACGGCGGACGGTGCAGAGGCCCGGGCGCT
  CCTCCGCTGTCGCCGCGCTGGATATCTGCCCCAGTTGTCTTTTTGTGGACTATGCTTCCAACTGCTTATA
  TATGGAGAATTGAAGGCTCAGTGACTGTTCGAAGATTATATTCAGTCCACTATGGAAGACTGAAAAAACC
  TCCCAGGTTCTCTCACTTAGCCAAGACAATTGGGCCAGTTTTGCTTCGAATGCACCGAATGAAAGACCTC
  ATCATGGTGCCCTACCCACCCTCCAACATTGCCTTCTGAGCCCCCTTGGACCAGGCTGAACCATTTGGTC
  TTATAGACTAGGCCTGATCCATCGCAGCACTCCAGAGTTTGGAATTGAGACCTTATGTCTCTGGAAGGCC
  TTCTGATCCATTCCCACCATGGACTGTTTGACCCTTCGGAGGACCTCTACACTCTCTCAAGCACGCATGT
  CAGAAATATCGAATTGCCTGGAGAAAGTCTCAGTGGTCTAGTGTAACCCAACTGGACTTCCCGA
  
  Kinase domain sequence

  >SC323584 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  ASCCAKTTCGTGACGCTTCAGGGCCGCGCGGCGGTGATCAAGCACCGCTTCCCCAAGGGCTACCSSYWCC
  CGGCGCTGGAGGCGCGGCTTGGCAGACGGCGGACGGTGCAGGAGGCCCGGGCGCTCCTCCGCTGTCGCCG
  CGCTGGAATATCTGCCCCAGTTGTCTTTTTTGTGGACTATGCTTCCAACTGCTTATATATGGAAGAAATT
  GAAGGCTCAGTGACTGTTCGAGATTATATTCAGTCCACTATGGAG
  
• Restriction Sites: Please inquire
• ACCN: NM_033550
• ORF Size: 762 bp
• Insert Size: 3180
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_033550.3, NP_291028.3
• RefSeq Size: 3384
• RefSeq ORF: 762
• Locus ID: 112858
• Domains: S_TKc, KOW
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. TP53RK has ATPase activity in the context of the EKC/KEOPS complex and likely plays a supporting role to the catalytic subunit OSGEP (By similarity). Atypical protein kinase that phosphorylates 'Ser-15' of p53/TP53 protein and may therefore participate in its activation. [UniProtKB/Swiss-Prot Function]