MARK3 (NM_002376) Human Untagged Clone

CAT#: SC323594

MARK3 (untagged)-Kinase deficient mutant (K85M) of Human MAP/microtubule affinity-regulating kinase 3 (MARK3), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MARK3
• Synonyms: CTAK1; KP78; Par-1a; PAR1A; VIPB
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323594 sequence for NM_002376 edited (data generated by NextGen Sequencing)
  ATGTCCACTAGGACCCCATTGCCAACGGTGAATGAACGAGACACTGAAAACCACACGTCA
  CATGGAGATGGGCGTCAAGAAGTTACCTCTCGTACCAGCCGCTCAGGAGCTCGGTGTAGA
  AACTCTATAGCCTCCTGTGCAGATGAACAACCTCACATCGGAAACTACAGACTGTTGAAA
  ACAATCGGCAAGGGGAATTTTGCAAAAGTAAAATTGGCAAGACATATCCTTACAGGCAGA
  GAGGTTGCAATAATGATAATTGACAAAACTCAGTTGAATCCAACAAGTCTACAAAAGCTC
  TTCAGAGAAGTAAGAATAATGAAGATTTTAAATCATCCCAATATAGTGAAGTTATTCGAA
  GTCATTGAAACTGAAAAAACACTCTACCTAATCATGGAATATGCAAGTGGAGGTGAAGTA
  TTTGACTATTTGGTTGCACATGGCAGGATGAAGGAAAAAGAAGCAAGATCTAAATTTAGA
  CAGATTGTGTCTGCAGTTCAATACTGCCATCAGAAACGGATCGTACATCGAGACCTCAAG
  GCTGAAAATCTATTGTTAGATGCCGATATGAACATTAAAATAGCAGATTTCGGTTTTAGC
  AATGAATTTACTGTTGGCGGTAAACTCGACACGTTTTGTGGCAGTCCTCCATACGCAGCA
  CCTGAGCTCTTCCAGGGCAAGAAATATGACGGGCCAGAAGTGGATGTGTGGAGTCTGGGG
  GTCATTTTATACACACTAGTCAGTGGCTCACTTCCCTTTGATGGGCAAAACCTAAAGGAA
  CTGAGAGAGAGAGTATTAAGAGGGAAATACAGAATTCCCTTCTACATGTCTACAGACTGT
  GAAAACCTTCTCAAACGTTTCCTGGTGCTAAATCCAATTAAACGCGGCACTCTAGAGCAA
  ATCATGAAGGACAGGTGGATCAATGCAGGGCATGAAGAAGATGAACTCAAACCATTTGTT
  GAACCAGAGCTAGACATCTCAGACCAAAAAAGAATAGATATTATGGTGGGAATGGGATAT
  TCACAAGAAGAAATTCAAGAATCTCTTAGTAAGATGAAATACGATGAAATCACAGCTACA
  TATTTGTTATTGGGGAGAAAATCTTCAGAGCTGGATGCTAGTGATTCCAGTTCTAGCAGC
  AATCTTTCACTTGCTAAGGTTAGGCCGAGCAGTGATCTCAACAACAGTACTGGCCAGTCT
  CCTCACCACAAAGTGCAGAGAAGTGTTTCTTCAAGCCAAAAGCAAAGACGCTACAGTGAC
  CATGCTGGACCAGCTATTCCTTCTGTTGTGGCGTATCCGAAAAGGAGTCAGACCAGCACT
  GCAGATAGTGACCTCAAAGAAGATGGAATTTCCTCCCGGAAATCAAGTGGCAGTGCTGTT
  GGAGGAAAGGGAATTGCTCCAGCCAGTCCCATGCTTGGGAATGCAAGTAATCCTAATAAG
  GCGGATATTCCTGAACGCAAGAAAAGCTCCACTGTCCCTAGTAGTAACACAGCATCTGGT
  GGAATGACACGACGAAATACTTATGTTTGCAGTGAGAGAACTACAGCTGATAGACACTCA
  GTGATTCAGAATGGCAAAGAAAACAGCACTATTCCTGATCAGAGAACTCCAGTTGCTTCA
  ACACACAGTATCAGTAGTGCAGCCACCCCAGATCGAATCCGCTTCCCAAGAGGCACTGCC
  AGTCGTAGCACTTTCCACGGCCAGCCCCGGGAACGGCGAACCGCAACATATAATGGCCCT
  CCTGCCTCTCCCAGCCTGTCCCATGAAGCCACACCATTGTCCCAGACTCGAAGCCGAGGC
  TCCACTAATCTCTTTAGTAAATTAACTTCAAAACTCACAAGGAGTCGCAATGTATCTGCT
  GAGCAAAAAGATGAAAACAAAGAAGCAAAGCCTCGATCCCTACGCTTCACCTGGAGCATG
  AAAACCACTAGTTCAATGGATCCCGGGGACATGATGCGGGAAATCCGCAAAGTGTTGGAC
  GCCAATAACTGCGACTATGAGCAGAGGGAGCGCTTCTTGCTCTTCTGCGTCCACGGAGAT
  GGGCACGCGGAGAACCTCGTGCAGTGGGAAATGGAAGTGTGCAAGCTGCCAAGACTGTCT
  CTGAACGGGGTCCGGTTTAAGCGGATATCGGGGACATCCATAGCCTTCAAAAATATTGCT
  TCCAAAATTGCCAATGAGCTAAAGCTGTAA
  
  Clone variation with respect to NM_002376.5
  254 a=>t;255 a=>g;1229 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_002376 unedited
  ACCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
  ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGCCACGGCGCCTTTT
  CGGAACTGCCGTGGACTCGAGGACGCTGGTCGCCGGCCTCCTAGGGCTGTGCTGTTTTGTTTTGACCCTC
  GCATTGTGCAGAATTAAAGTGCAGTAAAATGTCCACTAGGACCCCATTGCCAACGGTGAATGAACGAGAC
  ACTGAAAACCACACGTCACATGGAGATGGGCGTCAAGAAGTTACCTCTCGTACCAGCCGCTCAGGGAGCT
  CGGTGTAGAAACTCTATAGCCCTCCTGTGCAGATGAACAACCTCACATCGGAAACTACAGACTTGTTGAA
  AAACAATCGGCAAGGGGATTTTTGCAAAAAGTTAAAATTGCCAAGGACATATATCCTTTACCAGGCCAAG
  AGAGGTTGCAATAATGGATAATTGCACAAACTCTCGGTTGATTCACCCAAGTCTACAAAACCTCTCAGAG
  AAAGGTAGAAAAATGGAGAATTTTAAAACATCCCATTATATGTGAGTTTATCGGAGAGTTCATTGAAACT
  GAAAAACACCCCTCCCCCATATAACTTGAATATGCGCATGTGGGGGGTGGATAATTTGAGTATATTGGGT
  GTGGCACGGGGCGGGGAAGAGAGAAAAAAAGACCCGATCTTCTATTTTACCCAGTGTGGGTTGTGCATTT
  CTAATGCGCCTCAAAAGAGAGTGCTACTGGACACTAGGCGCAGAACTCTAGTGGTAGGCGCGTTGAGACA
  TTATATCGCATTCTGTTTTCACGAGATATCGTGTCGCGAACACACACGACTGTTGGGGACTCTACTACGC
  ACACTGATATCTAGAGAAATATCAGCGCATGAGTGTGATATCGGTCATATACACGCATGTGAATCTCTTT
  GTGGGACAC
  
  Kinase domain sequence

  >SC323594 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CGCTGCACATCGGCAGGGGATTTTGCAAAGTAAAATTGGCAAGACATATCCTTACAGGCAGAGAGGTTGC
  AATAATGATAATTGACAAAACTCAGTTGAATCCAACAAGTCTACAAAAGCTCTTCAGAGAAGTAAGAATA
  ATGAAGATTTTAAATCATCCCAATATAGTGAAGTTATTCGAAGTCATTGAAACTGAAAAAACACTCTACC
  TAATCATGGAATATGCAAGTGGAGGTGAAGTATTTGACTATTTGG
  
• Restriction Sites: Please inquire
• ACCN: NM_002376
• Insert Size: 2600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002376.4, NP_002367.4
• RefSeq Size: 3404 bp
• RefSeq ORF: 2190 bp
• Locus ID: 4140
• Cytogenetics: 14q32.32-q32.33
• Domains: UBA, pkinase, TyrKc, KA1, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: 'The protein encoded by this gene is activated by phosphorylation and in turn is involved in the phosphorylation of tau proteins MAP2 and MAP4. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]'
  Transcript Variant: This variant (3) lacks two alternate in-frame exons compared to variant 1. The resulting isoform (c) has the same N- and C-termini but is shorter compared to isoform a.