Germinal Center Kinase (MAP4K2) (NM_004579) Human Untagged Clone

CAT#: SC323630

MAP4K2 (untagged)-Kinase deficient mutant (K45M) of Human mitogen-activated protein kinase kinase kinase kinase 2 (MAP4K2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAP4K2
• Synonyms: BL44; GCK; RAB8IP
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004579, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGCTGCTGCGGGATGTGTCGCTGCAGGACCCGCGGGACCGCTTCGAGCTGCTGCAGCGCGTGGGGG
  CCGGGACCTATGGCGACGTCTACAAGGCCCGCGACACGGTCACGTCCGAACTGGCCGCCGTGAAGATAGT
  CAAGCTAGACCCAGGGGACGACATCAGCTCCCTCCAGCAGGAAATCACCATCCTGCGTGAGTGCCGCCAC
  CCCAATGTGGTGGCCTACATTGGCAGCTACCTCAGGAATGACCGCTTGTGGATCTGCATGGAGTTCTGCG
  GAGGGGGCTCCCTGCAGGAGATTTACCATGCCACTGGGCCCCTGGAGGAGCGGCAGATTGCCTACGTCTG
  CCGAGAGGCACTGAAGGGGCTCCACCACCTGCATTCTCAGGGGAAGATCCACAGAGACATCAAGGGAGCC
  AACCTTCTCCTCACTCTCCAGGGAGATGTCAAACTGGCTGACTTTGGGGTGTCAGGCGAGCTGACAGCGT
  CTGTGGCCAAGAGGAGGTCTTTCATTGGGACTCCCTACTGGATGGCTCCCGAGGTGGCTGCTGTGGAGCG
  CAAAGGTGGCTACAATGAGCTATGTGACGTCTGGGCCCTGGGCATCACTGCCATTGAGCTGGGCGAGCTG
  CAGCCCCCTCTGTTCCACCTGCACCCCATGAGGGCCCTGATGCTCATGTCGAAGAGCAGCTTCCAGCCGC
  CCAAACTGAGAGATAAGACTCGCTGGACCCAGAATTTCCACCACTTTCTCAAACTGGCCCTGACCAAGAA
  TCCTAAGAAGAGGCCGACAGCAGAGAAGCTCCTGCAGCACCCGTTCACGACTCAGCAGCTCCCTCGGGCC
  CTCCTCACACAGCTGCTGGACAAAGCCAGTGACCCTCATCTGGGGACCCCCTCCCCTGAGGACTGTGAGC
  TGGAGACCTATGACATGTTTCCAGACACCATTCACTCCCGGGGGCAGCACGGCCCAGCCGAGAGGACCCC
  CTCGGAGATCCAGTTTCACCAGGTGAAATTTGGCGCCCCACGCAGGAAGGAAACTGACCCACTGAATGAG
  CCGTGGGAGGAAGAGTGGACACTACTGGGAAAGGAAGAGTTGAGTGGGAGCCTGCTGCAGTCGGTCCAGG
  AGGCCCTGGAGGAAAGGAGTCTGACTATTCGGTCAGCCTCAGAATTCCAGGAGCTGGACTCCCCAGACGA
  TACCATGGGAACCATCAAGCGGGCCCCGTTCCTAGGGCCACTCCCCACTGACCCTCCAGCAGAGGAGCCT
  CTGTCCAGTCCCCCAGGAACCCTGCCCCCACCTCCTTCAGGCCCCAACAGCTCCCCACTGCTGCCCACGG
  CCTGGGCCACCATGAAGCAGCGGGAGGATCCTGAGAGGTCATCCTGCCACGGGCTCCCCCCAACTCCCAA
  GGTGCATATGGGCGCCTGCTTCTCCAAGGTCTTCAATGGCTGCCCCCTGCGGATCCACGCTGCTGTCACC
  TGGATTCACCCTGTTACTCGGGACCAGTTCCTGGTGGTAGGGGCCGAGGAAGGCATCTACACACTCAACC
  TGCATGAACTGCATGAGGATACGCTGGAGAAGCTGATTTCACATCGCTGCTCCTGGCTCTACTGCGTGAA
  CAACGTGCTGCTGTCACTCTCAGGGAAATCCACGCACATCTGGGCCCATGACCTCCCAGGCCTGTTTGAG
  CAGCGGAGGCTACAGCAACAGGTTCCCCTCTCCATCCCCACCAACCGCCTCACCCAGCGCATCATCCCCA
  GGCGCTTTGCTCTGTCCACCAAGATTCCTGACACCAAAGGCTGCTTGCAGTGTCGTGTGGTGCGGAACCC
  CTACACGGGTGCCACCTTCCTGCTGGCCGCCCTGCCCACCAGCCTGCTCCTGCTGCAGTGGTATGAGCCG
  CTGCAGAAGTTTCTGCTGCTGAAGAACTTCTCCAGCCCTCTGCCCAGCCCAGCTGGGATGCTGGAGCCGC
  TGGTGCTGGATGGGAAGGAGCTGCCGCAGGTGTGTGTTGGGGCCGAGGGGCCTGAGGGGCCCGGCTGCCG
  CGTCCTGTTCCATGTCCTGCCCCTGGAGGCTGGCCTGACGCCCGACATCCTCATCCCACCTGAGGGGATC
  CCAGGCTCGGCCCAGCAGGTGATCCAGGTGGACAGGGACACAATCCTAGTCAGCTTTGAACGCTGTGTGA
  GGATTGTCAACATGCAGGGCGAGCCCACGGCCACACTGGCACCTGAGCTGACCTTTGATTTCCCCATCGA
  GACTGTGGTGTGCCTGCAGGACAGTGTGCTGGCCTTCTGGAGCCATGGGATGCAAGGCCGAAGCCTGGAT
  ACCAATGAGGTGACCCAGGAGATCACAGATGAAACAAGGATCTTCCGAGTGCTTGGGGCCCACAGAGACA
  TCATCCTGGAGAGCATTCCCACTGACAACCCAGAGGCGCACAGCAACCTCTACATCCTCACGGGCCACCA
  GAGCACCTACTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_004579 unedited
  ACCCCCCCGCTTGAAAGCAATGGGCGGTAGGCGTGTACGGGGGGAGGTCTATATAAAGCAGCAGCTCGTT
  TAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCCCGGGATATCGTCGAC
  CCACGCGTCCGCAGAGCCACGGGCGCCCGCCCCGCCCCGCGCCGCCCCGCGCCGGCTCCGCAGCTCGCGC
  CCGCCCGCCTGCCGGCCCGCCCGGCGCCGGGCCATGGCGCTGCTGCAGGATGTGTCACTGCAGACCCGCG
  AACCGCTTCAGCTGCTGAGCGCGTGGGGGCCGGACCTATGCCACGTCTACAGCCCCGAACCGGTTAAGTC
  TCAACTGGCCACCTTAAGAAAGCCAACCACACCAGGGGAACAACATCGAAAACTC
  
  Kinase domain sequence

  >SC323630 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CYTTKGMGCAAATGGGCGKAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAYCGT
  CAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCCCGGGATATCGTCGACCCACGCGTCCG
  CAGAGCCACGGGCGCCCGCCCCGCCCCGCGCCGCCCCGCGCCGGCTCCGCAGCTCGCGCCCGCCCGCCTG
  CCGGCCCGCCCGGCGCCGGGCCATGGCGCTGCTGCGGGATGTGTC
  
• Restriction Sites: Please inquire
• ACCN: NM_004579
• ORF Size: 2463 bp
• Insert Size: 3000
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_004579.2, NP_004570.2
• RefSeq Size: 2964
• RefSeq ORF: 2463
• Locus ID: 5871
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: MAPK signaling pathway
• Gene Summary: The protein encoded by this gene is a member of the serine/threonine protein kinase family. Although this kinase is found in many tissues, its expression in lymphoid follicles is restricted to the cells of germinal centre, where it may participate in B-cell differentiation. This kinase can be activated by TNF-alpha, and has been shown to specifically activate MAP kinases. This kinase is also found to interact with TNF receptor-associated factor 2 (TRAF2), which is involved in the activation of MAP3K1/MEKK1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).