ARK5 (NUAK1) (NM_014840) Human Untagged Clone

CAT#: SC323652

NUAK1 (untagged)-Kinase deficient mutant (K84M) of Human NUAK family, SNF1-like kinase, 1 (NUAK1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NUAK1
• Synonyms: ARK5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323652 sequence for NM_014840 edited (data generated by NextGen Sequencing)
  ATGGAAGGGGCCGCCGCGCCTGTGGCGGGGGACCGCCCCGACTTGGGGCTGGGGGCGCCG
  GGCTCTCCCCGAGAGGCGGTGGCGGGGGCGACTGCAGCCCTGGAGCCCAGGAAGCCGCAC
  GGGGTGAAGCGGCATCACCACAAGCACAACTTGAAGCACCGCTACGAGCTGCAGGAGACC
  CTGGGCAAAGGCACCTACGGCAAAGTCAAGCGGGCCACCGAGAGGTTTTCTGGCCGAGTG
  GTTGCTATAATGTCCATTCGTAAGGACAAAATTAAGGATGAACAAGACATGGTTCACATC
  AGACGAGAGATTGAGATCATGTCATCTCTCAACCATCCTCATATCATCAGTATTTATGAA
  GTGTTTGAGAACAAAGATAAGATTGTGATCATCATGGAATATGCCAGCAAAGGGGAGCTG
  TACGATTACATCAGTGAGCGGCGACGCCTCAGTGAGAGGGAGACCCGGCACTTCTTCCGG
  CAGATCGTCTCTGCTGTGCACTATTGTCACAAGAACGGTGTGGTCCACCGGGACTTGAAG
  CTGGAAAATATACTGCTCGATGACAACTGCAATATTAAGATTGCTGACTTTGGGCTTTCC
  AACCTGTACCAGAAGGATAAGTTCTTACAAACGTTTTGTGGGAGTCCACTCTATGCATCT
  CCTGAGATTGTCAATGGGAGACCTTACCGAGGGCCAGAGGTGGACAGCTGGGCCCTGGGT
  GTGTTGCTTTACACTCTTGTTTATGGAACAATGCCCTTCGATGGTTTCGATCACAAAAAC
  CTCATTCGGCAAATCAGCAGCGGAGAGTACCGGGAGCCAACACAGCCCTCAGATGCTCGA
  GGACTCATACGGTGGATGCTGATGGTGAACCCCGATCGCCGGGCCACTATTGAGGACATT
  GCCAACCACTGGTGGGTGAACTGGGGCTATAAGAGCAGCGTGTGTGACTGTGATGCCCTC
  CATGACTCTGAGTCCCCACTCCTGGCTCGGATCATTGACTGGCACCACCGTTCCACAGGG
  CTGCAGGCTGACACCGAAGCCAAAATGAAGGGCCTGGCCAAACCCACGACCTCTGAGGTC
  ATGCTAGAGCGGCAGCGGTCGCTGAAGAAATCCAAGAAAGAGAATGACTTTGCTCAGTCT
  GGTCAGGATGCAGTGCCTGAAAGCCCATCCAAGTTGAGTTCTAAGAGGCCCAAGGGGATC
  CTGAAGAAGCGAAGCAACAGCGAGCATCGCTCTCACAGCACTGGCTTCATTGAAGGTGTA
  GTTGGTCCTGCCTTACCCTCTACTTTCAAGATGGAGCAGGACTTGTGCAGGACTGGCGTG
  CTCCTCCCAAGCTCACCAGAGGCAGAGGTGCCGGGAAAACTCAGCCCCAAGCAGTCGGCC
  ACGATGCCCAAGAAAGGCATCTTGAAAAAGACCCAGCAGAGAGAATCAGGTTACTACTCT
  TCCCCAGAGCGCAGTGAGTCTTCGGAGCTGTTGGACAGTAATGATGTGATGGGCAGCAGC
  ATCCCCTCCCCCAGCCCCCCGGACCCAGCCAGGGTAACCTCCCACAGCCTCTCCTGCCGG
  AGGAAGGGCATCTTGAAACACAGCAGCAAATACTCAGCGGGCACCATGGACCCAGCCCTG
  GTCAGCCCTGAAATGCCCACACTGGAATCCCTGTCAGAGCCTGGTGTCCCTGCCGAGGGC
  CTCTCCCGGAGCTACAGCCGCCCTTCCAGTGTCATCAGCGATGACAGCGTGCTGTCCAGC
  GACTCTTTTGACTTGCTGGATTTGCAGGAGAATCGCCCTGCCCGCCAGCGCATCCGCAGC
  TGCGTCTCTGCAGAAAACTTCCTCCAGATCCAGGACTTTGAGGGGCTCCAGAACCGGCCC
  CGGCCCCAGTACCTGAAGCGGTACCGGAACCGGCTGGCAGACAGCAGCTTCTCCCTCCTC
  ACAGACATGGATGATGTGACTCAGGTCTACAAGCAAGCGCTGGAGATCTGCAGCAAGCTC
  AACTAG
  
  Clone variation with respect to NM_014840.2
  251 a=>t;252 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_014840 unedited
  CCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGAGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC
  CGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCACCGCCGCCACC
  TCGCCCGACGCTCCACAGCTCGCCGCGGCCGGGGGGCGGTGCGCGGACCGTGCGCGCCGCGGGCGCCAGA
  TGTGCAGTCCCCGCCGCCGCCAGTGACCGAGCCGCAGTCCGAGCGGTATCGGGCCGCCTCCCTGATGCTG
  CGGGGGCGACCTTGAGCGTACAGCGGCTTCCCTCGGTGGGGACCCCGACATCCCAGCGCTGTGCCGCGGT
  GCTTGCCCTCTGTAGCCCGGCTCGCCCCGCGCTTGGGACATGGGAAGGGGCCCGCCGCCGCCTGGTGGCG
  GGGGGACCGCCCCCGAACTGGGGGCTGGGGGCCGCCGGGCTTCTCCCCGAAAGGCGTTGCCGGGGCCAAC
  TGCAGCCCTGGAGCCCAGAAGCCGCACGGGGGGAGCGGCATCACAAAGGCCCAACTTTGACCCCGCTCCG
  ACTGCAGAAACCTGGGCAAAGGCCTCACGGCAAATCAAGCGGCACCGAAGGTTTCTGGCAATGGGTGCTA
  AGTGCCTCTCAGAGCAAATTAGGTAGACAACA
  
  Kinase domain sequence

  >SC323652 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  TACKCAGRACCTGGGCAAGGCACCTACGGCAAAGTCAAGCGGGCCACCGAGAGGTTTTCTGGCCGAGTGG
  TTGCTATAATGTCCATTCGTAAGGACAAAATTAAGGATGAACAAGACATGGTTCACATCAGACGAGAGAT
  TGAGATCATGTCATCTCTCAACCATCCTCATATCATCAGTATTTATGAAGTGTTTGAGAACAAAGATAAG
  ATTGTGATCATCATGGAATATGCCAGCAAAGGGGAGCTGTACGAT
  
• Restriction Sites: Please inquire
• ACCN: NM_014840
• ORF Size: 1986 bp
• Insert Size: 2000
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Kinase deficit mutant (K84M)

Reference Data

• RefSeq: NM_014840.2, NP_055655.1
• RefSeq Size: 6821
• RefSeq ORF: 1986
• Locus ID: 9891
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase