COL8A1 (NM_020351) Human Untagged Clone

CAT#: SC323709

COL8A1 (untagged)-Human collagen, type VIII, alpha 1 (COL8A1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: COL8A1
• Synonyms: C3orf7
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_020351.2 CTCCGTGGGAGCCAGCGAGCCTCTCTCCCTGATCTTACGTGCTCAAGGGAGCTCACACGT
  TCACCAACTCACCCTTGAAGTCATCTCAAGAACAAAAGACAACTGAAAGAAGCTGTTGTG
  AAGGCAGAGCAGCATCTGCTGAAGAGACAGAAACCAGCCCCAGAGGTGTCACAGGAAGGC
  ACCAGCAAGGACATTGGTCTTTGATTTGATTCAGCAGTCCTGTCAAGTATAAATGTGATG
  GCTGTGCTGCCTGGCCCTCTGCAGCTGCTGGGAGTGCTGCTTACCATTTCCCTGAGTTCC
  ATCAGGCTCATTCAGGCTGGTGCCTACTATGGGATCAAGCCGCTGCCACCTCAAATTCCT
  CCTCAGATGCCACCACAAATTCCACAATACCAGCCCCTGGGTCAGCAAGTACCTCACATG
  CCTTTGGCCAAAGATGGCCTTGCCATGGGCAAGGAGATGCCCCACTTGCAGTATGGCAAA
  GAGTATCCACACCTACCCCAATATATGAAGGAAATTCAACCGGCGCCAAGAATGGGCAAG
  GAAGCCGTACCCAAGAAAGGCAAAGAAATACCATTAGCCAGTTTACGAGGGGAACAAGGT
  CCCCGTGGAGAGCCTGGCCCAAGAGGACCACCTGGGCCCCCTGGTTTGCCAGGTCATGGG
  ATACCTGGAATTAAAGGAAAACCAGGGCCACAGGGATATCCAGGAGTTGGAAAGCCAGGT
  ATGCCTGGAATGCCAGGGAAGCCAGGAGCCATGGGCATGCCTGGGGCAAAAGGAGAAATT
  GGACAGAAAGGGGAAATTGGGCCTATGGGGATCCCAGGACCACAAGGACCTCCAGGGCCT
  CATGGACTTCCTGGCATTGGGAAGCCAGGTGGGCCAGGGTTACCAGGGCAACCAGGACCA
  AAGGGTGATCGAGGACCCAAAGGACTACCAGGACCTCAAGGCCTTCGGGGTCCTAAAGGA
  GACAAGGGCTTCGGGATGCCAGGTGCGCCAGGTGTAAAGGGGCCTCCAGGGATGCACGGC
  CCTCCCGGCCCTGTTGGACTGCCAGGAGTGGGCAAACCAGGAGTGACAGGCTTCCCTGGG
  CCCCAGGGCCCCCTGGGAAAGCCAGGGGCTCCAGGAGAACCTGGGCCACAAGGCCCTATT
  GGGGTACCGGGGGTTCAAGGACCTCCTGGGATACCCGGAATTGGAAAGCCAGGCCAGGAT
  GGGATCCCAGGCCAGCCAGGATTTCCAGGTGGCAAAGGGGAGCAAGGACTGCCAGGGCTA
  CCAGGACCCCCAGGCCTTCCAGGGATTGGGAAACCAGGCTTCCCAGGACCCAAAGGTGAC
  CGGGGCATGGGAGGTGTTCCTGGGGCTCTTGGACCAAGAGGGGAGAAAGGACCAATAGGT
  GCCCCAGGAATAGGGGGTCCTCCAGGAGAGCCAGGCCTGCCTGGAATCCCAGGTCCTATG
  GGCCCTCCAGGTGCTATTGGTTTTCCTGGACCCAAAGGAGAAGGTGGGATTGTAGGGCCA
  CAGGGGCCACCAGGTCCCAAGGGTGAGCCAGGGCTTCAAGGCTTCCCAGGAAAGCCAGGT
  TTCCTTGGTGAAGTAGGGCCTCCTGGCATGAGGGGTTTGCCAGGTCCCATAGGGCCCAAG
  GGGGAAGCTGGGCAAAAAGGTGTACCAGGACTCCCTGGTGTTCCAGGGCTTCTCGGACCT
  AAGGGAGAGCCAGGAATCCCAGGGGATCAGGGTTTACAGGGCCCCCCAGGTATCCCAGGG
  ATTGGGGGCCCTAGTGGCCCCATTGGACCACCTGGGATTCCAGGCCCCAAAGGGGAGCCG
  GGCCTCCCAGGGCCCCCTGGGTTCCCTGGTATAGGGAAACCCGGAGTGGCAGGACTTCAT
  GGCCCCCCAGGGAAGCCTGGTGCCCTTGGTCCTCAAGGCCAGCCTGGCCTTCCAGGACCC
  CCAGGCCCTCCAGGACCTCCAGGACCCCCAGCTGTGATGCCCCCTACACCACCACCCCAG
  GGAGAGTATCTGCCAGATATGGGGCTGGGAATTGATGGCGTGAAACCCCCCCATGCCTAC
  GGGGCTAAGAAAGGCAAGAATGGAGGGCCAGCCTATGAGATGCCTGCATTTACCGCCGAG
  CTAACCGCACCTTTCCCACCGGTGGGGGCCCCAGTGAAGTTTAACAAACTGCTGTATAAC
  GGCAGACAGAACTACAACCCGCAGACAGGCATCTTCACCTGTGAGGTCCCTGGTGTCTAC
  TACTTTGCATACCACGTTCACTGCAAGGGGGGCAACGTGTGGGTTGCTCTATTCAAGAAC
  AACGAGCCCGTGATGTACACGTACGACGAGTACAAAAAGGGCTTCCTGGACCAGGCATCT
  GGGAGTGCAGTGCTGCTGCTCAGGCCCGGAGACCGGGTGTTCCTCCAGATGCCCTCAGAA
  CAGGCTGCAGGACTGTATGCCGGGCAGTATGTCCACTCCTCCTTTTCAGGATATTTATTG
  TATCCCATGTAAAAACAAAAAAACAAAAAACAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_020351
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020351.2, NP_065084.2
• RefSeq Size: 2614 bp
• RefSeq ORF: 2235 bp
• Locus ID: 1295
• Cytogenetics: 3q12.1
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes one of the two alpha chains of type VIII collagen. The gene product is a short chain collagen and a major component of the basement membrane of the corneal endothelium. The type VIII collagen fibril can be either a homo- or a heterotrimer. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Dec 2011]'
  Transcript Variant: This variant (2) lacks an exon in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein.