SATB1 (NM_001131010) Human Untagged Clone

CAT#: SC325321

SATB1 (untagged)-Human SATB homeobox 1 (SATB1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SATB1
• Synonyms: DNA-binding protein SATB1; SATB homeobox 1; scaffold-associating DNA's); special AT-rich sequence binding protein 1; special AT-rich sequence binding protein 1 (binds to nuclear matrix
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001131010, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGATCATTTGAACGAGGCAACTCAGGGGAAAGAACATTCAGAAATGTCTAACAATGTGAGTGATCCGA
  AGGGTCCACCAGCCAAGATTGCCCGCCTGGAGCAGAACGGGAGCCCGCTAGGAAGAGGAAGGCTTGGGAG
  TACAGGTGCAAAAATGCAGGGAGTGCCTTTAAAACACTCGGGCCATCTGATGAAAACCAACCTTAGGAAA
  GGAACCATGCTGCCAGTTTTCTGTGTGGTGGAACATTATGAAAACGCCATTGAATATGATTGCAAGGAGG
  AGCATGCAGAATTTGTGCTGGTGAGAAAGGATATGCTTTTCAACCAGCTGATCGAAATGGCATTGCTGTC
  TCTAGGTTATTCACATAGCTCTGCTGCCCAGGCCAAAGGGCTAATCCAGGTTGGAAAGTGGAATCCAGTT
  CCACTGTCTTACGTGACAGATGCCCCTGATGCTACAGTAGCAGATATGCTTCAAGATGTGTATCATGTGG
  TCACATTGAAAATTCAGTTACACAGTTGCCCCAAACTAGAAGACTTGCCTCCCGAACAATGGTCGCACAC
  CACAGTGAGGAATGCTCTGAAGGACTTACTGAAAGATATGAATCAGAGTTCATTGGCCAAGGAGTGCCCC
  CTTTCACAGAGTATGATTTCTTCCATTGTGAACAGTACTTACTATGCAAATGTCTCAGCAGCAAAATGTC
  AAGAATTTGGAAGGTGGTACAAACATTTCAAGAAGACAAAAGATATGATGGTTGAAATGGATAGTCTTTC
  TGAGCTATCCCAGCAAGGCGCCAATCATGTCAATTTTGGCCAGCAACCAGTTCCAGGGAACACAGCCGAG
  CAGCCTCCATCCCCTGCGCAGCTCTCCCATGGCAGCCAGCCCTCTGTCCGGACACCTCTTCCAAACCTGC
  ACCCTGGGCTCGTATCAACACCTATCAGTCCTCAATTGGTCAACCAGCAGCTGGTGATGGCTCAGCTGCT
  GAACCAGCAGTATGCAGTGAATAGACTTTTAGCCCAGCAGTCCTTAAACCAACAATACTTGAACCACCCT
  CCCCCTGTCAGTAGATCTATGAATAAGCCTTTGGAGCAACAGGTTTCGACCAACACAGAGGTGTCTTCCG
  AAATCTACCAGTGGGTACGCGATGAACTGAAACGAGCAGGAATCTCCCAGGCGGTATTTGCACGTGTGGC
  TTTTAACAGAACTCAGGGCTTGCTTTCAGAAATCCTCCGAAAGGAAGAGGACCCCAAGACTGCATCCCAG
  TCTTTGCTGGTAAACCTTCGGGCTATGCAGAATTTCTTGCAGTTACCGGAAGCTGAAAGAGACCGAATAT
  ACCAGGACGAAAGGGAAAGGAGCTTGAATGCTGCCTCGGCCATGGGTCCTGCCCCCCTCATCAGCACACC
  ACCCAGCCGTCCTCCCCAGGTGAAAACAGCTACTATTGCCACTGAAAGGAATGGGAAACCAGAGAACAAT
  ACCATGAACATTAATGCTTCCATTTATGATGAGATTCAGCAGGAAATGAAGCGTGCTAAAGTGTCTCAAG
  CACTGTTTGCAAAGGTTGCAGCAACCAAAAGCCAGGGATGGTTGTGCGAGCTGTTACGCTGGAAAGAAGA
  TCCTTCTCCAGAAAACAGAACCCTGTGGGAGAACCTCTCCATGATCCGAAGGTTCCTCAGTCTTCCTCAG
  CCAGAACGTGATGCCATTTATGAACAGGAGAGCAACGCGGTGCATCACCATGGCGACAGGCCGCCCCACA
  TTATCCATGTTCCAGCAGAGCAGATTCAGCAACAGCAGCAGCAACAGCAACAGCAGCAGCAGCAGCAGCA
  GGCACCGCCGCCTCCACAGCCACAGCAGCAGCCACAGACAGGCCCTCGGCTCCCCCCACGGCAACCCACG
  GTGGCCTCTCCAGCAGAGTCAGATGAGGAAAACCGACAGAAGACCCGGCCACGAACAAAAATTTCAGTGG
  AAGCCTTGGGAATCCTCCAGAGTTTCATACAAGACGTGGGCCTGTACCCTGACGAAGAGGCCATCCAGAC
  TCTGTCTGCCCAGCTCGACCTTCCCAAGTACACCATCATCAAGTTCTTTCAGAACCAGCGGTACTATCTC
  AAGCACCACGGCAAACTGAAGGACAATTCCGGTTTAGAGGTCGATGTGGCAGAATATAAAGAAGAGGAGC
  TGCTGAAGGATTTGGAAGAGAGTGTCCAAGATAAAAATACTAACACCCTTTTTTCAGTGAAACTAGAAGA
  AGAGCTGTCAGTGGAAGGAAACACAGACATTAATACTGATTTGAAAGACTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001131010
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001131010.3, NP_001124482.1
• RefSeq Size: 6307 bp
• RefSeq ORF: 2292 bp
• Locus ID: 6304
• Cytogenetics: 3p24.3
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'This gene encodes a matrix protein which binds nuclear matrix and scaffold-associating DNAs through a unique nuclear architecture. The protein recruits chromatin-remodeling factors in order to regulate chromatin structure and gene expression. [provided by RefSeq, Apr 2016]'
  Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate exon in the 3' coding region, compared to variant 3. Isoform 1 has a shorter C-terminus, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.