ARFGAP3 (NM_001142293) Human Untagged Clone

CAT#: SC325971

ARFGAP3 (untagged)-Human ADP-ribosylation factor GTPase activating protein 3 (ARFGAP3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ARFGAP3
• Synonyms: ARFGAP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001142293, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGGGACCCCAGCAAGCAGGACATCTTGACCATCTTCAAGCGCCTCCGCTCGGTGCCCACTAACAAGG
  TGTGTTTTGATTGTGGTGCCAAAAATCCCAGCTGGGCAAGCATAACCTATGGAGTGTTCCTTTGCATTGA
  TTGCTCAGGGTCCCACCGGTCACTTGGTGTTCACTTGAGTTTTATTCGATCTACAGAGTTGGATTCCAAC
  TGGTCATGGTTTCAGTTGCGATGCATGCAAGTCGGAGGAAACGCTAGTGCACTGTGGCTTGATAGTTGTG
  TGGTTCCACCTTTGTCCCCTCCACCAAAGGAGGAAGATTTTTTTGCCTCTCACGTTTCTCCTGAGGTGAG
  TGACACAGCGTGGGCATCAGCAATAGCAGAACCATCTTCTTTAACATCAAGGCCTGTGGAAACCACTTTG
  GAAAATAATGAAGGTGGACAAGAGCAAGGACCAAGTGTGGAAGGTCTTAATGTACCAACAAAGGCTACTT
  TAGAGGTATCCTCTATCATAAAAAAGAAACCAAATCAAGCTAAAAAAGGCCTTGGGGCCAAAAAAGGAAG
  TTTGGGAGCTCAGAAACTGGCAAACACATGCTTTAATGAAATTGAAAAACAAGCTCAAGCTGCGGATAAA
  ATGAAGGAGCAGGAAGACCTGGCCAAGGTGGTATCTAAAGAAGAATCAATTGTTTCATCATTACGATTAG
  CCTATAAGGATCTTGAAATTCAAATGAAGAAAGACGAAAAGATGAACATTAGTGGCAAAAAAAATGTTGA
  CTCAGACAGACTCGGCATGGGATTTGGAAATTGCAGAAGTGTTATTTCACATTCAGTGACTTCAGATATG
  CAGACCATAGAGCAGGAATCACCCATTATGGCAAAACCAAGAAAAAAGTATAATGATGACAGTGACGATT
  CATATTTTACTTCCAGCTCAAGTTACTTTGACGAGCCAGTGGAGTTAAGGAGCAGTTCTTTCTCTAGCTG
  GGATGACAGTTCAGATTCCTATTGGAAAAAAGAGACCAGCAAAGATACTGAAACAGTTCTGAAAACCACA
  GGCTATTCAGACAGACCTACTGCTCGCCGCAAGCCAGATTATGAGCCAGTTGAAAATACAGATGAGGCCC
  AGAAGAAGTTTGGCAATGTCAAGGCCATTTCATCAGATATGTATTTTGGAAGACAATCCCAGGCTGATTA
  TGAGACCAGGGCCCGCCTAGAGAGGCTGTCGGCAAGTTCCTCCATAAGCTCGGCTGATCTGTTCGAGGAG
  CCGAGGAAGCAGCCAGCAGGGAACTACAGCCTGTCCAGTGTGCTGCCCAACGCCCCCGACATGGCGCAGT
  TCAAGCAGGGAGTGAGATCGGTTGCTGGAAAACTCTCCGTCTTTGCTAATGGAGTCGTGACTTCAATTCA
  GGATCGCTACGGTTCTTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001142293
• ORF Size: 1419 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001142293.1, NP_001135765.1
• RefSeq Size: 2719
• RefSeq ORF: 1419
• Locus ID: 26286
• Protein Pathways: Endocytosis
• Gene Summary: The protein encoded by this gene is a GTPase-activating protein (GAP) that associates with the Golgi apparatus and regulates the early secretory pathway of proteins. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1 (ARF1)-bound GTP, which is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids. Multiple transcript variants encoding different isoforms have been found for this gene. This gene was originally known as ARFGAP1, but that is now the name of a related but different gene. [provided by RefSeq, Nov 2008]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. The resulting protein (isoform 2) has the same N- and C-termini but is shorter than isoform 1.