ATF6 beta (ATF6B) (NM_001136153) Human Untagged Clone

CAT#: SC326184

ATF6B (untagged)-Human activating transcription factor 6 beta (ATF6B), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATF6B
• Synonyms: CREB-RP; CREBL1; G13
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001136153, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGAGCTGATGCTGCTCAGCGAGATTGCTGACCCGACGCGTTTCTTCACCGACAACCTGCTTAGCC
  CGGAGGACTGGGACAGCACCTTGTATTCTGGCCTAGATGAAGTGGCCGAGGAGCAGACGCAGCTCTTCCG
  TTGCCCGGAGCAGGATGTCCCGTTTGACGGCAGCTCCCTGGACGTGGGGATGGATGTCAGCCCCTCTGAG
  CCCCCATGGGAACTCCTGCCGATCTTCCCAGATCTTCAGGTGAAGTCTGAGCCATCTTCCCCCTGCTCTT
  CCTCCTCCCTCAGCTCCGAGTCATCGCGTCTCTCCACAGAGCCATCCAGCGAGGCTCTTGGGGTAGGGGA
  GGTGCTCCATGTGAAGACAGAGTCCTTGGCACCCCCACTGTGTCTCCTGGGAGATGACCCAACATCCTCA
  TTTGAAACCGTCCAGATCAACGTTATCCCCACCTCTGATGATTCCTCAGATGTCCAGACCAAGATAGAAC
  CTGTCTCTCCATGTTCTTCCGTCAACTCTGAGGCCTCCCTGCTCTCAGCCGACTCCTCCAGCCAGGCTTT
  TATAGGAGAGGAGGTCCTGGAAGTGAAGACAGAGTCCCTGTCCCCTTCAGGATGCCTCCTGTGGGATGTC
  CCAGCCCCCTCACTTGGAGCTGTCCAGATCAGCATGGGCCCATCCCTTGATGGCTCCTCAGGCAAAGCCC
  TGCCCACCCGGAAGCCGCCACTGCAGCCCAAACCTGTAGTGCTAACCACTGTCCCAATGCCATCCAGAGC
  TGTGCCTCCCAGCACCACAGTCCTTCTGCAGTCCCTCGTCCAGCCACCCCCAGTGTCCCCAGTTGTCCTC
  ATCCAGGGTGCTATTCGAGTCCAGCCTGAAGGGCCGGCTCCCTCTCTACCACGGCCTGAGAGGAAGAGCA
  TCGTTCCCGCTCCTATGCCTGGAAACTCCTGCCCGCCTGAAGTGGATGCAAAGCTGCTGAAGCGGCAGCA
  GCGAATGATCAAGAACCGGGAGTCAGCCTGCCAGTCCCGGAGAAAGAAGAAAGAGTATCTGCAGGGACTG
  GAGGCTCGGCTGCAAGCAGTACTGGCTGACAACCAGCAGCTCCGCCGAGAGAATGCTGCCCTCCGGCGGC
  GGCTGGAGGCCCTGCTGGCTGAAAACAGCGAGCTCAAGTTAGGGTCTGGAAACAGGAAGGTGGTCTGCAT
  CATGGTCTTCCTTCTCTTCATTGCCTTCAACTTTGGACCTGTCAGCATCAGTGAGCCTCCTTCAGCTCCC
  ATCTCTCCTCGGATGAACAAGGGGGAGCCTCAACCCCGGAGACACTTGCTGGGGTTCTCAGAGCAAGAGC
  CAGTTCAGGGAGTTGAACCTCTCCAGGGGTCCTCCCAGGGCCCTAAGGAGCCCCAGCCCAGCCCCACAGA
  CCAGCCCAGTTTCAGCAACCTGACAGCCTTCCCTGGGGGCGCCAAGGAGCTACTACTAAGAGACCTAGAC
  CAGCTCTTCCTCTCCTCTGATTGCCGGCACTTCAACCGCACTGAGTCCCTGAGGCTTGCTGACGAGTTGA
  GTGGCTGGGTCCAGCGCCACCAGAGAGGCCGGAGGAAGATCCCTCAGAGGGCCCAGGAGAGACAGAAGTC
  TCAGCCACGGAAGAAGTCACCTCCAGTTAAGGCAGTCCCCATCCAACCCCCTGGACCCCCAGAAAGGGAT
  TCTGTGGGCCAGCTGCAACTATATCGCCACCCAGACCGTTCGCAGCCAGCATTCTTGGATGCAATTGACC
  GACGGGAAGACACATTTTATGTTGTCTCTTTCCGAAGGGACCACCTGCTGCTCCCAGCCATCAGCCACAA
  CAAGACCTCCCGGCCCAAGATGTCCCTGGTGATGCCTGCCATGGCCCCCAATGAGACCCTGTCAGGCCGT
  GGGGCCCCGGGGGACTATGAGGAGATGATGCAGATCGAGTGTGAGGTCATGGACACCAGGGTGATTCACA
  TCAAGACCTCCACAGTGCCCCCCTCGCTCCGAAAACAGCCATCCCCAACCCCAGGCAATGCCACAGGTGG
  CCCCTTGCCAGTCTCTGCAGCCAGCCAGGCCCACCAGGCCTCCCACCAGCCCCTCTACCTCAATCATCCC
  TGA
  
  
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001136153
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001136153.1, NP_001129625.1
• RefSeq Size: 2613 bp
• RefSeq ORF: 2103 bp
• Locus ID: 1388
• Cytogenetics: 6p21.32
• Protein Families: Transcription Factors
• Gene Summary: 'The protein encoded by this gene is a transcription factor in the unfolded protein response (UPR) pathway during ER stress. Either as a homodimer or as a heterodimer with ATF6-alpha, the encoded protein binds to the ER stress response element, interacting with nuclear transcription factor Y to activate UPR target genes. The protein is normally found in the membrane of the endoplasmic reticulum; however, under ER stress, the N-terminal cytoplasmic domain is cleaved from the rest of the protein and translocates to the nucleus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]'
  Transcript Variant: This variant (2) uses an alternate in-frame splice site at the 3' end of exon 1 compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is 3 aa shorter compared to isoform a.