TRPC4 (NM_003306) Human Untagged Clone

CAT#: SC326306

TRPC4 (untagged)-Human transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant epsilon

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TRPC4
• Synonyms: HTRP-4; HTRP4; TRP4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003306, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTCAGTTCTATTACAAAAGAAATGTTAATGCTCCCTATAGAGACCGCATCCCTCTAAGGATAGTAA
  GAGCAGAATCAGAACTCTCGCCATCAGAAAAAGCCTACTTGAATGCTGTGGAAAAGGGAGATTATGCCAG
  TGTCAAGAAATCCCTAGAGGAAGCTGAAATTTATTTTAAAATCAATATTAATTGCATTGATCCTCTCGGA
  AGAACTGCTCTCCTCATTGCAATTGAAAATGAGAACTTGGAGCTCATCGAACTACTCTTAAGCTTTAATG
  TCTATGTTGGAGATGCTCTATTACATGCTATCAGAAAAGAAGTCGTCGGAGCTGTTGAGCTGTTATTGAA
  CCACAAAAAACCTAGTGGAGAAAAACAGGTGCCTCCTATACTCCTTGATAAGCAGTTCTCTGAATTCACT
  CCAGACATTACACCAATCATTTTGGCAGCCCATACAAATAATTATGAGATAATAAAACTCTTGGTTCAGA
  AAGGAGTCTCAGTGCCTCGACCCCACGAGGTCCGCTGTAACTGTGTGGAATGCGTGTCCAGTTCAGATGT
  GGACAGCCTCCGTCACTCACGCTCCAGACTCAACATCTACAAGGCCTTGGCCAGTCCCTCTCTCATTGCA
  CTGTCAAGCGAAGATCCTTTTCTCACAGCCTTTCAGTTAAGTTGGGAACTTCAGGAACTGAGCAAGGTGG
  AAAATGAATTCAAGTCGGAGTATGAAGAGCTGTCACGGCAGTGCAAACAATTTGCTAAGGACCTACTGGA
  TCAGACGAGAAGTTCCAGAGAACTGGAAATCATTCTTAATTACCGAGATGACAATAGTCTCATAGAAGAA
  CAAAGTGGAAATGATCTTGCAAGACTAAAATTGGCCATTAAGTACCGTCAAAAAGAGTTTGTTGCCCAGC
  CCAATTGTCAACAGCTGCTGGCATCTCGCTGGTACGATGAGTTTCCAGGCTGGAGGAGAAGACACTGGGC
  AGTGAAGATGGTGACATGTTTCATAATAGGACTTCTTTTTCCTGTCTTCTCTGTGTGCTACCTGATAGCT
  CCCAAAAGCCCACTTGGACTGTTCATCAGGAAGCCATTTATCAAGTTTATCTGCCACACAGCCTCCTATT
  TGACTTTTTTGTTCCTGCTGCTGCTTGCCTCTCAGCACATCGACAGGTCAGACTTGAACAGGCAAGGTCC
  ACCACCAACCATCGTCGAGTGGATGATATTACCGTGGGTCCTGGGCTTCATATGGGGAGAAATTAAACAG
  ATGTGGGATGGCGGACTTCAGGACTACATCCATGATTGGTGGAATCTAATGGACTTTGTAATGAACTCCT
  TATATTTAGCAACAATCTCCTTGAAAATTGTTGCATTTGTAAAGTACAGTGCCCTTAATCCACGAGAATC
  ATGGGACATGTGGCATCCCACTCTGGTGGCAGAGGCTTTATTTGCTATTGCAAACATCTTCAGTTCTCTG
  CGTCTGATCTCACTGTTTACTGCAAATTCTCACCTGGGACCTCTGCAAATATCTCTGGGAAGAATGCTCC
  TGGACATTTTGAAGTTTCTATTCATATACTGCCTTGTGTTGCTAGCATTTGCAAATGGCCTAAATCAATT
  GTACTTCTATTATGAAGAAACGAAAGGGTTAACCTGCAAAGGCATAAGATGTGAAAAGCAGAATAATGCA
  TTTTCAACGTTATTTGAGACACTGCAGTCCCTGTTTTGGTCAATATTTGGGCTCATCAATTTATATGTGA
  CCAATGTCAAAGCACAGCATGAATTTACTGAGTTTGTTGGTGCCACCATGTTTGGGACATACAATGTCAT
  CTCTCTGGTTGTTCTACTCAACATGTTAATAGCTATGATGAATAATTCTTACCAACTGATTGCTGACCAT
  GCAGATATAGAATGGAAATTTGCACGAACAAAGCTTTGGATGAGTTATTTTGAAGAAGGAGGTACTCTGC
  CTACTCCCTTCAATGTCATCCCGAGCCCCAAGTCTCTCTGGTACCTGATCAAATGGATCTGGACACACTT
  GTGCAAGAAAAAGATGAGAAGAAAGCCAGAAAGTTTTGGAACAATAGGGGTAAGAACACAGCATAGGCGA
  GCTGCTGATAACTTGAGAAGACATCACCAATACCAAGAAGTTATGAGGAACCTGGTGAAGCGATACGTTG
  CTGCAATGATTAGAGATGCTAAAACTGAAGAAGGCCTGACCGAAGAGAACTTTAAGGAACTAAAGCAAGA
  CATTTCTAGTTTCCGCTTTGAAGTCCTGGGATTACTAAGAGGAAGCAAACTTTCCACAATACAATCTGCG
  AATGCCTCGAAGGAGTCTTCAAATTCGGCAGACTCAGATGAAAAGAGTGATAGCGAAGGTAATAGCAAGG
  ACAAGAAAAAGAATTTCAGCCTTTTTGATTTAACCACCCTGATTCATCCGAGATCAGCAGCAATTGCCTC
  TGAAAGACATAACATAAGCAATGGCTCTGCCCTGGTGGTTCAGGAGCCGCCCAGGGAGAAGCAGAGAAAA
  GTGAATTTTGTGACCGATATCAAAAACTTTGGGTTATTTCATAGACGATCAAAACAAAATGCTGCTGAGC
  AAAATGCAAACCAAATCTTCTCTGTTTCAGAAGAAGTTGCTCGTCAACAGGCTGCAGGACCACTTGAGAG
  AAATATTCAACTGGAATCTCGAGGATTAGCTTCACGGGGTGACCTGAGCATTCCCGGTCTCAGTGAACAA
  TGTGTGTTAGTAGACCATAGAGAAAGGAATACGGACACACTGGGGTTACAGGTAGGAAAGAGAGTGTGTC
  CATTCAAGTCAGAGAAGGTGGTGGTGGAGGACACGGTTCCTATAATACCAAAGGAGAAACATGCAAAAGA
  AGAGGACTCTAGTATAGACTATGATCTAAACCTCCCAGACACAGTCACCCACGAAGATTACGTGACCACA
  AGATTGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_003306
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003306.1, NP_003297.1
• RefSeq Size: 3549 bp
• RefSeq ORF: 2949 bp
• Locus ID: 7223
• Cytogenetics: 13q13.3
• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
• Gene Summary: 'This gene encodes a member of the canonical subfamily of transient receptor potential cation channels. The encoded protein forms a non-selective calcium-permeable cation channel that is activated by Gq-coupled receptors and tyrosine kinases, and plays a role in multiple processes including endothelial permeability, vasodilation, neurotransmitter release and cell proliferation. Single nucleotide polymorphisms in this gene may be associated with generalized epilepsy with photosensitivity. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]'
  Transcript Variant: This variant (5, also known as epsilon) uses a different splice site, compared to variant 1 (alpha). The encoded isoform (epsilon) is longer, compared to isoform alpha. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.