Glycogen synthase 1 (GYS1) (NM_001161587) Human Untagged Clone

CAT#: SC327628

GYS1 (untagged)-Human glycogen synthase 1 (muscle) (GYS1) transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GYS1
• Synonyms: GSY; GYS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001161587, the custom clone sequence may differ by one or more nucleotides
  ATGCCTTTAAACCGCACTTTGTCCATGTCCTCACTGCCAGGACTGGAGGACTGGGAGGAT
  GAATTCGACCTGGAGAACGCAGTGCTCTTCGAAGTGGCCTGGGAGGTGGCTAACAAGGTG
  GGTGGCATCTACACGGTGCTGCAGACGAAGGCGAAGGTGACAGGGGACGAATGGGGCGAC
  AACTACTTCCTGGTGGGGCCGTACACGGAGCAGGGCGTGAGGACCCAGGTGGAACTGCTG
  GAGGCCCCCACCCCGGCCCTGAAGAGGACACTGGATTCCATGAACAGCAAGGGCTGCAAG
  TTCCTGGCACAGAGTGAGGAGAAGCCACATGTGGTTGCTCACTTCCATGAGTGGTTGGCA
  GGCGTTGGACTCTGCCTGTGTCGTGCCCGGCGACTGCCTGTAGCAACCATCTTCACCACC
  CATGCCACGCTGCTGGGGCGCTACCTGTGTGCCGGTGCCGTGGACTTCTACAACAACCTG
  GAGAACTTCAACGTGGACAAGGAAGCAGGGGAGAGGCAGATCTACCACCGATACTGCATG
  GAAAGGGCGGCAGCCCACTGCGCTCACGTCTTCACTACTGTGTCCCAGATCACCGCCATC
  GAGGCACAGCACTTGCTCAAGAGGAAACCAGATATTGTGACCCCCAATGGGCTGAATGTG
  AAGAAGTTTTCTGCCATGCATGAGTTCCAGAACCTCCATGCTCAGAGCAAGGCTCGAATC
  CAGGAGTTTGTGCGGGGCCATTTTTATGGGCATCTGGACTTCAACTTGGACAAGACCTTA
  TACTTCTTTATCGCCGGCCGCTATGAGTTCTCCAACAAGGGTGCTGACGTCTTCCTGGAG
  GCATTGGCTCGGCTCAACTATCTGCTCAGAGTGAACGGCAGCGAGCAGACAGTGGTTGCC
  TTCTTCATCATGCCAGCGCGGACCAACAATTTCAACGTGGAAACCCTCAAAGGCCAAGCT
  GTGCGCAAACAGCTTTGGGACACGGCCAACACGGTGAAGGAAAAGTTCGGGAGGAAGCTT
  TATGAATCCTTACTGGTTGGGAGCCTTCCCGACATGAACAAGATGCTGGATAAGGAAGAC
  TTCACTATGATGAAGAGAGCCATCTTTGCAACGCAGCGGCAGTCTTTCCCCCCTGTGTGC
  ACCCACAATATGCTGGATGACTCCTCAGACCCCATCCTGACCACCATCCGCCGAATCGGC
  CTCTTCAATAGCAGTGCCGACAGGGTGAAGGTGATTTTCCACCCGGAGTTCCTCTCCTCC
  ACAAGCCCCCTGCTCCCTGTGGACTATGAGGAGTTTGTCCGTGGCTGTCACCTTGGAGTC
  TTCCCCTCCTACTATGAGCCTTGGGGCTACACACCGGCTGAGTGCACGGTTATGGGAATC
  CCCAGTATCTCCACCAATCTCTCCGGCTTCGGCTGCTTCATGGAGGAACACATCGCAGAC
  CCCTCAGCTTACGGTATCTACATTCTTGACCGGCGGTTCCGCAGCCTGGATGATTCCTGC
  TCGCAGCTCACCTCCTTCCTCTACAGTTTCTGTCAGCAGAGCCGGCGGCAGCGTATCATC
  CAGCGGAACCGCACGGAGCGCCTCTCCGACCTTCTGGACTGGAAATACCTAGGCCGGTAC
  TATATGTCTGCGCGCCACATGGCGCTGTCCAAGGCCTTTCCAGAGCACTTCACCTACGAG
  CCCAACGAGGCGGATGCGGCCCAGGGGTACCGCTACCCACGGCCAGCCTCGGTGCCACCG
  TCGCCCTCGCTGTCACGACACTCCAGCCCGCACCAGAGTGAGGACGAGGAGGATCCCCGG
  AACGGGCCGCTGGAGGAAGACGGCGAGCGCTACGATGAGGACGAGGAGGCCGCCAAGGAC
  CGGCGCAACATCCGTGCACCAGAGTGGCCGCGCCGAGCGTCCTGCACCTCCTCCACCAGC
  GGCAGCAAGCGCAACTCTGTGGACACGGCCACCTCCAGCTCACTCAGCACCCCGAGCGAG
  CCCCTCAGCCCCACCAGCTCCCTGGGCGAGGAGCGTAAC
  
• Restriction Sites: Please inquire
• ACCN: NM_001161587
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001161587.1, NP_001155059.1
• RefSeq Size: 3443 bp
• RefSeq ORF: 2022 bp
• Locus ID: 2997
• Cytogenetics: 19q13.33
• Protein Pathways: Insulin signaling pathway, Starch and sucrose metabolism
• Gene Summary: 'The protein encoded by this gene catalyzes the addition of glucose monomers to the growing glycogen molecule through the formation of alpha-1,4-glycoside linkages. Mutations in this gene are associated with muscle glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]'
  Transcript Variant: This variant (2) is missing an in-frame coding exon at the 3' end compared to variant 1, resulting in a shorter isoform (2) lacking an internal protein segment compared to isoform 1.