CASQ1 (NM_001231) Human Untagged Clone

CAT#: SC327824

CASQ1 (untagged)-Human calsequestrin 1 (fast-twitch skeletal muscle) (CASQ1) nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CASQ1
• Synonyms: CASQ; PDIB1; VMCQA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001231, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGTGCTACAGACAGGATGGGGCCCAGAGCTGTGCCGGGTCTGCGGCTGGCACTGCTGTTGCTGCTGG
  TGCTAGGGACACCCAAGTCAGGGGTACAGGGGCAGGAAGGGCTGGACTTCCCTGAGTACGATGGTGTGGA
  CCGTGTGATCAATGTCAATGCAAAGAACTACAAGAATGTGTTCAAGAAGTATGAGGTGCTGGCACTCCTC
  TACCATGAACCCCCCGAGGATGACAAGGCCTCACAAAGACAATTTGAGATGGAGGAGCTGATCCTGGAGT
  TAGCAGCCCAAGTCCTAGAAGACAAGGGTGTTGGCTTCGGGCTGGTAGACTCTGAGAAGGATGCAGCTGT
  GGCCAAGAAACTAGGCCTAACTGAAGTGGACAGCATGTATGTATTCAAGGGAGATGAAGTCATTGAGTAC
  GATGGCGAGTTTTCTGCTGACACCATCGTGGAGTTTCTGCTTGATGTCCTAGAGGACCCTGTGGAATTGA
  TTGAAGGTGAACGAGAGCTGCAGGCGTTTGAGAATATTGAGGATGAGATCAAACTCATTGGCTACTTCAA
  GAGCAAAGACTCAGAGCATTACAAAGCCTTCGAGGATGCAGCTGAGGAGTTTCATCCCTACATCCCCTTC
  TTCGCCACCTTCGACAGCAAGGTGGCAAAGAAGCTGACCCTGAAGCTGAATGAGATTGATTTCTACGAGG
  CCTTCATGGAAGAGCCTGTGACCATCCCAGACAAGCCCAATAGCGAAGAGGAGATTGTCAACTTCGTGGA
  GGAGCACAGGAGATCAACCCTGAGGAAACTGAAGCCGGAGAGTATGTATGAGACCTGGGAGGATGATATG
  GATGGAATCCACATTGTGGCCTTCGCAGAGGAAGCTGATCCTGATGGTTTCGAGTTCTTAGAGACTCTCA
  AGGCTGTGGCCCAAGATAACACTGAAAACCCAGATCTTAGCATCATCTGGATTGACCCTGATGACTTCCC
  CCTGCTGGTCCCATACTGGGAGAAGACGTTTGACATCGACTTGTCAGCCCCACAAATAGGAGTCGTCAAT
  GTTACTGATGCGGATAGCGTATGGATGGAAATGGACGATGAGGAGGACCTGCCTTCTGCTGAGGAGCTGG
  AGGACTGGCTGGAGGATGTCCTGGAGGGCGAGATCAACACAGAGGACGATGACGATGATGATGATGACTA
  G
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001231
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001231.4, NP_001222.3
• RefSeq Size: 1993 bp
• RefSeq ORF: 1191 bp
• Locus ID: 844
• Cytogenetics: 1q23.2
• Domains: Calsequestrin
• Gene Summary: 'This gene encodes the skeletal muscle specific member of the calsequestrin protein family. Calsequestrin functions as a luminal sarcoplasmic reticulum calcium sensor in both cardiac and skeletal muscle cells. This protein, also known as calmitine, functions as a calcium regulator in the mitochondria of skeletal muscle. This protein is absent in patients with Duchenne and Becker types of muscular dystrophy. [provided by RefSeq, Jun 2013]'