DNAJC7 (NM_003315) Human Untagged Clone

CAT#: SC327881

DNAJC7 (untagged)-Human DnaJ (Hsp40) homolog subfamily C member 7 (DNAJC7) transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DNAJC7
• Synonyms: DJ11; DJC7; TPR2; TTC2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003315, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGCTGCCGCGGAGTGCGATGTGGTAATGGCGGCGACCGAGCCGGAGCTGCTCGACGACCAAGAGG
  CGAAGAGGGAAGCAGAGACTTTCAAGGAACAAGGAAATGCATACTATGCCAAGAAAGATTACAATGAAGC
  TTATAATTATTATACAAAAGCCATAGATATGTGTCCTAAAAATGCTAGCTATTATGGTAATCGAGCAGCC
  ACCTTGATGATGCTTGGAAGGTTCCGGGAAGCTCTTGGAGATGCACAACAGTCAGTGAGGTTGGATGACA
  GTTTTGTCCGGGGACATCTACGAGAGGGCAAGTGCCACCTCTCTCTGGGGAATGCCATGGCAGCATGTCG
  CAGCTTCCAGAGAGCCCTAGAACTGGATCATAAAAATGCTCAGGCACAACAAGAGTTCAAGAATGCTAAT
  GCAGTCATGGAATATGAGAAAATAGCAGAAACAGATTTTGAGAAGCGAGATTTTCGGAAGGTTGTTTTCT
  GCATGGACCGTGCCCTAGAATTTGCCCCTGCCTGCCATCGCTTCAAAATCCTCAAGGCAGAATGTTTAGC
  AATGCTGGGTCGTTATCCAGAAGCACAGTCTGTGGCTAGTGACATTCTACGAATGGATTCCACCAATGCA
  GATGCTCTGTATGTACGAGGTCTTTGCCTTTATTACGAAGATTGTATTGAGAAGGCAGTTCAGTTTTTCG
  TACAGGCTCTCAGGATGGCTCCTGACCACGAGAAGGCCTGCATTGCCTGCAGAAATGCCAAAGCACTCAA
  AGCAAAGAAAGAAGATGGGAATAAAGCATTTAAGGAAGGAAATTACAAACTAGCATATGAACTGTACACA
  GAAGCCCTGGGGATAGACCCCAACAATATAAAAACAAATGCTAAACTCTACTGTAATCGGGGTACGGTTA
  ATTCCAAGCTTAGGAAACTAGATGATGCAATAGAAGACTGCACAAATGCAGTGAAGCTTGATGACACTTA
  CATAAAAGCCTACTTGAGAAGAGCTCAGTGTTACATGGACACAGAACAGTATGAAGAAGCAGTACGAGAC
  TATGAAAAAGTATACCAGACAGAGAAAACAAAAGAACACAAACAGCTCCTAAAAAATGCGCAGCTGGAAC
  TGAAGAAGAGTAAGAGGAAAGATTACTACAAGATTCTAGGAGTGGACAAGAATGCCTCTGAGGACGAGAT
  CAAGAAAGCTTATCGGAAACGGGCCTTGATGCACCATCCAGATCGGCATAGTGGAGCCAGTGCTGAGGTT
  CAGAAGGAGGAGGAGAAGAAGTTCAAGGAAGTTGGAGAGGCCTTTACTATCCTCTCTGATCCCAAGAAAA
  AGACTCGCTATGACAGTGGACAGGACCTAGATGAGGAGGGCATGAATATGGGTGATTTTGATCCAAACAA
  TATCTTCAAGGCATTCTTTGGCGGTCCTGGCGGCTTCAGCTTTGAAGCATCTGGTCCAGGGAATTTCTTT
  TTTCAATTTGGCTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_003315
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003315.3, NP_003306.3
• RefSeq Size: 2096 bp
• RefSeq ORF: 1485 bp
• Locus ID: 7266
• Cytogenetics: 17q21.2
• Domains: TPR, DnaJ
• Gene Summary: 'This gene encodes a member of the DNAJ heat shock protein 40 family of proteins that is characterized by two N-terminal tetratricopeptide repeat domains and a C-terminal DNAJ domain. This protein binds the chaperone proteins heat shock proteins 70 and 90 in an ATP-dependent manner and may function as a co-chaperone. Pseudogenes of this gene are found on chromosomes 1 and 6. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2009]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).