ZFX (NM_001178084) Human Untagged Clone

CAT#: SC329141

ZFX (untagged)-Human zinc finger protein X-linked (ZFX) transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ZFX
• Synonyms: ZNF926
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001178084, the custom clone sequence may differ by one or more nucleotides
  ATGGATGAAGATGGGCTTGAATTACAACAAGAGCCAAACTCATTTTTTGATGCAACAGGA
  GCTGATGGTACACACATGGATGGTGATCAAATTGTTGTGGAAGTACAAGAAACTGTTTTT
  GTTTCAGATGTTGTGGATTCAGACATAACTGTGCATAACTTTGTTCCTGATGACCCAGAT
  TCAGTTGTAATCCAAGATGTTATTGAGGACGTTGTTATAGAAGATGTTCAGTGCCCAGAT
  ATCATGGAAGAAGCAGATGTGTCTGAAACGGTCATCATTCCTGAGCAAGTGCTGGACTCA
  GATGTAACTGAAGAAGTTTCTTTAGCACATTGCACAGTCCCAGATGATGTTTTAGCTTCT
  GACATTACTTCAGCCTCAATGTCTATGCCAGAACACGTCTTGACGGGTGATTCTATACAT
  GTGTCTGACGTTGGACATGTTGGACATGTTGGACATGTTGAACATGTGGTTCATGATAGT
  GTAGTGGAAGCAGAAATTGTCACTGATCCTCTGACTACCGACGTAGTTTCAGAAGAAGTA
  TTGGTAGCAGACTGTGCCTCTGAAGCAGTCATAGATGCCAATGGGATCCCTGTGGACCAG
  CAGGATGATGACAAAGGCAACTGTGAGGACTACCTTATGATTTCCTTGGATGATGCTGGC
  AAAATAGAACACGATGGTTCTTCTGGAATGACCATGGACACAGAGTCGGAAATTGATCCT
  TGTAAAGTGGATGGCACTTGCCCTGAGGTCATCAAGGTGTACATTTTTAAAGCTGACCCT
  GGAGAAGATGACTTAGGTGGAACTGTAGACATTGTGGAGAGTGAGCCTGAGAATGATCAT
  GGAGTTGAACTGCTTGATCAGAACAGCAGTATTCGTGTTCCCAGGGAAAAGATGGTTTAT
  ATGACTGTCAATGACTCTCAGCCAGAAGATGAAGATTTAAATGTTGCTGAAATCGCTGAC
  GAAGTTTATATGGAAGTGATCGTAGGAGAGGAGGATGCTGCAGCAGCAGCGGCAGCCGCC
  GCCGTGCACGAGCAGCAAATGGATGACAATGAAATCAAAACCTTCATGCCGATTGCATGG
  GCAGCAGCTTATGGTAATAATTCTGATGGAATTGAAAACCGGAATGGCACTGCAAGTGCC
  CTCTTGCACATAGATGAGTCTGCTGGCCTCGGCAGACTGGCTAAACAAAAACCAAAGAAA
  AGGAGAAGACCTGATTCCAGGCAGTACCAAACAGCAATAATTATTGGCCCTGATGGACAT
  CCTTTGACTGTCTATCCTTGCATGATTTGTGGGAAGAAGTTTAAGTCGAGAGGTTTTTTG
  AAAAGGCACATGAAAAACCATCCCGAACACCTTGCCAAGAAGAAATACCGCTGTACTGAC
  TGTGATTACACTACCAACAAGAAGATAAGTTTACACAACCACCTGGAGAGCCACAAGCTG
  ACCAGCAAGGCAGAGAAGGCCATTGAATGCGATGAGTGTGGGAAGCATTTCTCTCATGCA
  GGGGCTTTGTTTACTCACAAAATGGTGCATAAGGAAAAAGGAGCCAACAAAATGCACAAG
  TGTAAATTCTGTGAATACGAGACAGCTGAACAAGGGTTATTGAATCGCCACCTCTTGGCA
  GTCCACAGCAAGAACTTTCCTCATATTTGTGTGGAGTGTGGTAAGGGTTTTCGTCACCCG
  TCAGAGCTCAAAAAGCACATGAGAATCCATACTGGGGAGAAGCCGTACCAATGCCAGTAC
  TGCGAATATAGGTCTGCAGACTCTTCTAACTTGAAAACGCATGTCAAAACTAAGCATAGT
  AAAGAGATGCCATTCAAGTGTGACATTTGTCTTCTGACTTTCTCGGATACCAAAGAGGTG
  CAGCAACATGCTCTTATCCACCAAGAAAGCAAAACACACCAGTGTTTGCATTGCGACCAC
  AAGAGTTCGAACTCAAGTGATTTGAAACGACACATAATTTCAGTTCACACGAAAGACTAC
  CCCCATAAGTGTGACATGTGTGATAAAGGCTTTCACAGGCCTTCAGAACTCAAGAAACAC
  GTGGCTGCCCACAAGGGCAAAAAAATGCACCAGTGTAGACATTGTGACTTTAAGATTGCA
  GATCCATTTGTTCTAAGTCGCCATATTCTCTCAGTTCACACAAAGGATCTTCCATTTAGG
  TGCAAGAGATGTAGAAAGGGATTTAGGCAACAGAGTGAGCTTAAAAAGCATATGAAGACA
  CACAGTGGCAGGAAAGTGTATCAGTGTGAGTACTGTGAGTATAGCACTACAGATGCCTCA
  GGCTTTAAACGGCACGTTATTTCCATTCACACGAAAGACTATCCTCACCGGTGTGAGTAC
  TGCAAGAAAGGCTTCCGAAGACCTTCAGAAAAGAACCAGCACATAATGCGACATCATAAA
  GAAGTTGGCCTGCCCTAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001178084
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001178084.1, NP_001171555.1
• RefSeq Size: 7396 bp
• RefSeq ORF: 2418 bp
• Locus ID: 7543
• Cytogenetics: Xp22.11
• Protein Families: Transcription Factors
• Gene Summary: 'This gene on the X chromosome is structurally similar to a related gene on the Y chromosome. It encodes a member of the krueppel C2H2-type zinc-finger protein family. The full-length protein contains an acidic transcriptional activation domain (AD), a nuclear localization sequence (NLS) and a DNA binding domain (DBD) consisting of 13 C2H2-type zinc fingers. Studies in mouse embryonic and adult hematopoietic stem cells showed that this gene was required as a transcriptional regulator for self-renewal of both stem cell types, but it was dispensable for growth and differentiation of their progeny. Multiple alternatively spliced transcript variants encoding different isoforms have been identified, but the full-length nature of some variants has not been determined. [provided by RefSeq, May 2010]'
  Transcript Variant: This variant (2) lacks two exons in the 5' UTR, as compared to variant 1. Variants 1, 2, and 3 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.