MEST (NM_001253901) Human Untagged Clone

CAT#: SC332251

MEST (untagged) - Homo sapiens mesoderm specific transcript (MEST), transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MEST
• Synonyms: PEG1
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001253901, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGGGAGTGGTGGGTCCAGGTGGGGCTGCTGGCCGTGCCCCTGCTTGCTGCGTACCTGCACATCCCAC
  CCCCTCAGCTCTCCCCTGCCCTTCACTCATGGAAGTCTTCAGGCAAGTTTTTCACTTACAAGGGACTGCG
  TATCTTCTACCAAGACTCTGTGGGTGTGGTTGGAAGTCCAGAGATAGTTGTGCTTTTACACGGTTTTCCA
  ACATCCAGCTACGACTGGTACAAGATTTGGGAAGGTCTGACCTTGAGGTTTCATCGGGTGATTGCCCTTG
  ATTTCTTAGGCTTTGGCTTCAGTGACAAACCGAGACCACATCACTATTCCATATTTGAGCAGGCCAGCAT
  CGTGGAAGCGCTTTTGCGGCATCTGGGGCTCCAGAACCGCAGGATCAACCTTCTTTCTCATGACTATGGA
  GATATTGTTGCTCAGGAGCTTCTCTACAGGTACAAGCAGAATCGATCTGGTCGGCTTACCATAAAGAGTC
  TCTGTCTGTCAAATGGAGGTATCTTTCCTGAGACTCACCGTCCACTCCTTCTCCAAAAGCTACTCAAAGA
  TGGAGGTGTGCTGTCACCCATCCTCACACGACTGATGAACTTCTTTGTATTCTCTCGAGGTCTCTTACAG
  TACATCAATCAGAGGAAGAAGTTCAGAAGGCGCTGGGTGGGAGCTCTTGCCTCTGTAACTATCCCCATTC
  ATTTTATCTATGGGCCATTGGATCCTGTAAATCCCTATCCAGAGTTTTTGGAGCTGTACAGGAAAACGCT
  GCCGCGGTCCACAGTGTCGATTCTGGATGACCACATTAGCCACTATCCACAGCTAGAGGATCCCATGGGC
  TTCTTGAATGCATATATGGGCTTCATCAACTCCTTCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001253901
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001253901.1, NP_001240830.1
• RefSeq Size: 2350 bp
• RefSeq ORF: 879 bp
• Locus ID: 4232
• Cytogenetics: 7q32.2
• Protein Families: Protease, Transmembrane
• Gene Summary: 'This gene encodes a member of the alpha/beta hydrolase superfamily. It is imprinted, exhibiting preferential expression from the paternal allele in fetal tissues, and isoform-specific imprinting in lymphocytes. The loss of imprinting of this gene has been linked to certain types of cancer and may be due to promotor switching. The encoded protein may play a role in development. Alternatively spliced transcript variants encoding multiple isoforms have been identified for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3 and 4, and the long arm of chromosomes 6 and 15. [provided by RefSeq, Dec 2011]'
  Transcript Variant: This variant (5) differs in the 5' UTR, lacks an exon in the coding region and uses a downstream, in-frame start codon, compared to variant 1. Variants 5 and 6 encode the same isoform (d), which is shorter than isoform a.