DDX11 (NM_001257145) Human Untagged Clone

CAT#: SC332507

DDX11 (untagged) - Homo sapiens DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11 (DDX11), transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DDX11
• Synonyms: CHL1; CHLR1; KRG2; WABS
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001257145, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCAGAGCTGTACCGGGTTTTGGAGGCTGGCAAGATTGGGATATTTGAGAGTCCAACTGGCACTGGGA
  AGTCCTTAAGTCTTATTTGTGGGGCCCTCTCTTGGCTCCGTGACTTTGAACAGAAGAAGCGTGAAGAAGA
  GGCACGACTCCTTGAAACTGGAACTGGCCCCTTACATGATGAGAAAGATGAATCCCTGTGTCTGTCTTCT
  TCCTGCGAAGGGGCTGCAGGCACCCCGAGGCCTGCTGGAGAACCGGCCTGGGTTACTCAGTTTGTGCAGA
  AGAAAGAAGAGAGGGACCTGGTGGACCGACTAAAGGCGGAGCAGGCCAGGAGGAAGCAGCGAGAAGAACG
  CCTGCAGCAGCTGCAGCACAGGGTGCAGCTCAAGTATGCAGCCAAGCGCCTGAGGCAGGAAGAAGAAGAA
  AGAGAGAATCTCCTCCGCCTCAGCAGGGAGATGCTAGAGACAGGCCCGGAGGCTGAGCGGCTGGAGCAGC
  TGGAGTCTGGGGAGGAGGAGCTGGTCCTCGCCGAATACGAGAGTGATGAGGAGAAAAAGGTGGCGAGCAG
  AGTGGATGAGGATGAGGATGACCTGGAGGAAGAACACATAACTAAGATTTATTACTGTAGTCGGACACAC
  TCCCAGCTGGCCCAGTTTGTGCATGAGGTGAAGAAGAGCCCCTTTGGCAAGGATGTTCGGCTGGTCTCCC
  TTGGCTCCCGGCAGAACCTTTGTGTAAATGAAGACGTGAAAAGCCTAGGTTCTGTGCAGCTTATCAACGA
  CCGCTGTGTGGACATGCAGAGAAGCAGGCACGAGAAGAAGAAAGGAGCTGAGGAGGAGAAGCCAAAGAGG
  AGGAGGCAGGAGAAGCAGGCAGCCTGCCCCTTCTACAACCACGAGCAGATGGGCCTTCTCCGGGATGAGG
  CCCTGGCAGAGGTGAAGGACATGGAGCAGCTGCTGGCCCTTGGGAAGGAGGCCCGGGCCTGTCCCTATTA
  CGGGAGCCGCCTTGCCATCCCTGCAGCCCAGCTGGTGGTGCTGCCCTATCAGATGCTGCTGCATGCGGCC
  ACTCGGCAGGCCGCGGGCATCCGGCTGCAGGACCAGGTGGTGATCATCGACGAGGCGCACAACCTGATCG
  ACACCATCACGGGCATGCACAGCGTGGAGGTCAGCGGCTCCCAGCTCTGCCAGGCCCATTCCCAGCTGCT
  GCAGTACGTGGAGCGATACGGGAAGCGTTTGAAGGCCAAGAACCTGATGTACCTGAAGCAGATCCTGTAT
  TTGCTGGAGAAATTCGTGGCTGTGCTAGGGGGGAACATTAAGCAAAATCCCAATACACAGAGTCTGTCAC
  AGACAGGGACGGAGCTGAAGACCATCAACGACTTTCTCTTCCAGAGCCAGATCGACAACATCAACCTGTT
  CAAGGTGCAGCGATACTGTGAGAAGAGCATGATCAGCAGAAAGCTCTTTGGATTCACTGAACGGTACGGA
  GCAGTGTTCTCATCCCGGGAGCAGCCCAAACTGGCTGGGTTTCAGCAATTCCTGCAGAGCCTGCAGCCCA
  GGACGACTGAAGCTCTTGCAGCCCCTGCAGACGAGAGTCAGGCCAGCACCCTGCGACCAGCTTCTCCACT
  GATGCACATCCAAGGCTTCCTGGCAGCTCTCACTACGGCCAACCAGGACGGCAGGGTCATCCTGAGCCGC
  CAAGGCAGCCTCAGTCAGAGCACCCTGAAGTTTTTGCTCCTGAATCCAGCTGTGCACTTTGCCCAAGTGG
  TGAAGGAATGCCGGGCAGTGGTCATTGCGGGGGGTACCATGCAGCCGGTGTCTGACTTCCGGCAGCAGCT
  GCTGGCCTGTGCCGGGGTGGAAGCTGAGCGCGTGGTGGAGTTTTCCTGTGGTCACGTGATCCCTCCAGAC
  AACATCCTGCCCCTCGTCATCTGCAGCGGGATCTCCAACCAGCCGCTGGAATTCACGTTCCAGAAAAGAG
  AGCTGCCTCAGATGATGGACGAGGTGGGTCGCATTCTCTGTAACCTGTGCGGTGTGGTTCCTGGAGGGGT
  GGTCTGTTTCTTCCCCTCCTACGAGTACCTGCGCCAGGTCCATGCCCACTGGGAGAAGGGTGGCCTGCTG
  GGCCGTCTGGCTGCCAGGAAGAAGATATTCCAGGAACCTAAGAGCGCACACCAGGTGGAGCAGGTGCTGC
  TGGCATATTCCAGGTGCATCCAGGCCTGTGGCCAGGAGAGAGGCCAGGTGACAGGGGCCCTGCTCCTCTC
  TGTGGTTGGAGGAAAGATGAGTGAAGGGATCAACTTCTCTGACAACCTAGGCCGGTGTGTGGTGATGGTG
  GGCATGCCCTTCCCCAACATCAGGTCTGCAGAGCTGCAGGAGAAGATGGCCTACTTGGATCAAACCCTCC
  CCAGAGCCCCCGGCCAGGCACCCCCAGGGAAGGCTCTGGTGGAGAACCTGTGCATGAAGGCCGTCAACCA
  GTCCATAGGCAGGGCCATCAGGCACCAGAAGGATTTTGCCAGCGTAGTGCTCCTGGACCAGCGATATGCC
  CGGCCCCCTGTCCTGGCCAAGCTGCCGGCCTGGATCCGAGCCCGTGTGGAGGTCAAAGCTACCTTTGGCC
  CCGCCATTGCTGCTGTGCAGAAGTTTCACCGGGAGAAGTCGGCCTCTTCCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001257145
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001257145.1, NP_001244074.1
• RefSeq Size: 3737 bp
• RefSeq ORF: 2643 bp
• Locus ID: 1663
• Cytogenetics: 12p11.21
• Protein Families: Stem cell - Pluripotency
• Gene Summary: 'DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an enzyme that possesses both ATPase and DNA helicase activities. This gene is a homolog of the yeast CHL1 gene, and may function to maintain chromosome transmission fidelity and genome stability. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (5) differs in the 5' UTR and coding region, and uses a downstream AUG compared to variant 3. The resulting protein (isoform 4) has shorter N- and C-termini compared to isoform 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.