VSTM2A (NM_001301009) Human Untagged Clone

CAT#: SC334742

VSTM2A (untagged) - Human V-set and transmembrane domain containing 2A (VSTM2A), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VSTM2A
• Synonyms: VSTM2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC334742 representing NM_001301009.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGATGGGGATCTTTTTGGTGTATGTTGGATTTGTTTTCTTTTCCGTTTTATATGTACAACAAGGGCTT
  TCTTCTCAAGCAAAATTTACCGAGTTTCCGCGGAACGTGACGGCGACCGAGGGGCAGAATGTGGAGATG
  TCCTGCGCCTTCCAGAGCGGCTCCGCCTCGGTGTATCTGGAGATCCAATGGTGGTTCCTGCGGGGGCCG
  GAGGACCTGGATCCCGGGGCCGAGGGGGCCGGCGCGCAGGTGGAGCTCTTGCCCGACAGAGACCCGGAC
  AGCGACGGGACCAAGATCAGCACAGTGAAAGTCCAAGGCAATGACATCTCCCACAAGCTTCAGATTTCC
  AAAGTGAGGAAAAAGGATGAAGGCTTATATGAGTGCAGGGTGACTGATGCCAACTACGGGGAGCTTCAG
  GAACACAAGGCCCAGGCCTATCTGAAAGTCAATGCCAACAGCCATGCCCGCAGAATGCAGGCCTTCGAA
  GCCTCGCCCATGTGGCTGCAGGATATGAAGCCCCGCAAGAACGTCTCCGCAGCCATCCCCAGCAGCATC
  CATGGCTCTGCCAACCAACGAACGCACTCCACCTCCAGCCCTCAAGTGGTAGCCAAAATCCCCAAACAA
  AGTCCACAATCAGCAAAGAGCAAATCGCCTGTAAAATCTACGGAGCGGACAGCAAAGTTGACCCTAAAC
  TCCAAGCACCACCCTGCACCCACTGTACTCTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI Plasmid Map
• ACCN: NM_001301009
• Insert Size: 723 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001301009.1
• RefSeq Size: 3000 bp
• RefSeq ORF: 723 bp
• Locus ID: 222008
• Cytogenetics: 7p11.2
• Protein Families: Secreted Protein, Transmembrane
• MW: 26.5 kDa
• Gene Summary: Plays a role in the regulation of the early stage of white and brown preadipocyte cell differentiation. Promotes adipogenic commitment of preadipocytes by increasing gene expression of the transcription factor PPARG in a BMP4-dependent signaling pathway.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 3' coding region due to the use of an alternate splice site in the 3'-terminal exon, compared to variant 1. The encoded isoform (2) has a longer and distinct C-terminus, compared to isoform 1.