VTA1 (NM_001286371) Human Untagged Clone

CAT#: SC335033

VTA1 (untagged) - Human vesicle (multivesicular body) trafficking 1 (VTA1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VTA1
• Synonyms: C6orf55; DRG-1; DRG1; HSPC228; LIP5; My012; SBP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC335033 representing NM_001286371.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCCGCGCTTGCACCGCTGCCCCCGCTCCCCGCACAGTTCAAGAGCATACAGCATCATCTGAGGACG
  GCTCAGGAGCATGACAAGCGAGACCCTGTGGTGGCTTATTACTGTCGTTTATACGCAATGCAGACTGGA
  ATGAAGATCGATAGTAAAACTCCTGAATGTCGCAAATTTTTATCAAAGTTAATGGATCAGTTAGAAGCT
  CTAAAGAAGCAGTTGGGTGATAATGAAGCTATTACTCAAGAAATAGTGGGCTGTGCCCATTTGGAGAAT
  TATGCTTTGAAAATGTTTTTGTATGCAGACAATGAAGATCGTGCTGGACGATTTCACAAAAACATGATC
  AAGTCCTTCTATACTGCAAGTCTTTTGATAGATGTCATAACAGTATTTGGAGAACTCACTGATGAAAAT
  GTGAAACACAGGAAGTATGCCAGATGGAAGGCAACATACATCCATAATTGTTTAAAGAATGGGGAGACT
  CCTCAAGCAGGCCCTGTTGGAATTGAAGAAGATAATGATATTGAAGAAAATGAAGATGCTGGAGCAGCC
  TCTCTGCCCACTCAGCCAACTCAGCCATCATCATCTTCAACTTATGACCCAAGCAACATGCCATCAGGC
  AACTATACTGGAATACAGATTCCTCCGGGTGCACACGCTCCAGCTAATACACCAGCAGAAGTGCCTCAC
  AGCACAGGGGATGTTCGTCTAACCCCAGAAGACTTTGCTAGAGCTCAGAAGTACTGCAAATATGCTGGC
  AGTGCTTTGCAGTATGAAGATGTAAGCACTGCTGTCCAGAATCTACAAAAGGCTCTCAAGTTACTGACG
  ACAGGCAGAGAATGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI Plasmid Map
• ACCN: NM_001286371
• Insert Size: 843 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286371.1
• RefSeq Size: 3290 bp
• RefSeq ORF: 843 bp
• Locus ID: 51534
• UniProt ID: Q9NP79
• Cytogenetics: 6q24.1-q24.2
• Protein Pathways: Endocytosis
• MW: 31.1 kDa
• Gene Summary: C6ORF55 encodes a protein involved in trafficking of the multivesicular body, an endosomal compartment involved in sorting membrane proteins for degradation in lysosomes (Ward et al., 2005 [PubMed 15644320]).[supplied by OMIM, Mar 2008]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a.