HARS2 (NM_001278732) Human Untagged Clone

CAT#: SC335622

HARS2 (untagged) - Human histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HARS2
• Synonyms: HARSL; HARSR; HisRS; HO3; PRLTS2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001278732, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGTTGTGAGGGAGAAAATTCTTGATTTGGTTATCAGCTGCTTTAAACGTCATGGAGCAAAGGGGATGG
  ACACCCCAGCATTTGAGCTGAAGGATTTTGACATTGCTGGTCAGTTTGACCCTATGATCCCCGATGCAGA
  GTGTTTGAAGATCATGTGTGAAATCCTAAGTGGATTGCAGTTGGGAGACTTTCTCATTAAGGTAAATGAC
  CGGCGGATTGTGGATGGGATGTTTGCTGTCTGTGGTGTTCCTGAAAGCAAGTTCCGTGCCATCTGCTCCT
  CCATAGATAAACTAGACAAGATGGCTTGGAAAGATGTGAGACATGAGATGGTGGTGAAGAAAGGCCTGGC
  TCCTGAGGTGGCTGATCGAATTGGGGACTATGTCCAGTGTCATGGTGGGGTATCCCTAGTAGAGCAAATG
  TTTCAGGATCCCAGACTATCCCAGAACAAGCAGGCCCTGGAGGGCCTGGGAGACCTAAAGCTGCTATTTG
  AATACCTGACTTTATTTGGAATTGCTGATAAGATCTCCTTTGACCTCAGCCTGGCTCGGGGCCTAGACTA
  CTATACAGGAGTGATCTATGAAGCAGTGCTGCTGCAGACCCCAACTCAGGCTGGGGAGGAGCCCCTGAAT
  GTGGGCAGTGTGGCTGCTGGTGGGCGCTATGATGGGCTGGTGGGCATGTTTGACCCCAAGGGCCACAAGG
  TGCCATGTGTGGGACTCAGCATTGGGGTTGAGCGAATCTTCTACATTGTGGAGCAGAGGATGAAGACCAA
  AGGTGAGAAGGTGCGGACTACAGAGACTCAAGTGTTTGTGGCCACACCACAGAAGAACTTTCTCCAAGAA
  CGGTTGAAGCTTATTGCAGAGCTTTGGGATTCTGGAATCAAGGCAGAGATGCTATACAAGAACAACCCCA
  AACTATTAACCCAGCTGCACTATTGTGAGAGCACAGGCATTCCACTGGTGGTCATTATTGGTGAGCAAGA
  ACTGAAAGAAGGGGTCATCAAGATCCGTTCAGTGGCCAGCAGAGAGGAGGTGGCCATTAAACGGGAAAAT
  TTTGTGGCTGAAATTCAGAAGCGACTGTCTGAGTCTTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001278732
• ORF Size: 1089 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_001278732.1, NP_001265661.1
• RefSeq Size: 2393
• RefSeq ORF: 1089
• Locus ID: 23438
• Protein Pathways: Aminoacyl-tRNA biosynthesis
• Gene Summary: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is an enzyme belonging to the class II family of aminoacyl-tRNA synthetases. Functioning in the synthesis of histidyl-transfer RNA, the enzyme plays an accessory role in the regulation of protein biosynthesis. The gene is located in a head-to-head orientation with HARS on chromosome five, where the homologous genes likely share a bidirectional promoter. Mutations in this gene are associated with the pathogenesis of Perrault syndrome, which involves ovarian dysgenesis and sensorineural hearing loss. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]
  Transcript Variant: This variant (3) uses an alternate splice site and lacks two alternate in-frame exons in the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 3, which is shorter than isoform 1. This isoform (3) lacks the transit peptide present in isoforms 1 and 2, hence it is not likely to localize to the mitochondrion.